Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 780,929 C→T intergenic (+11/‑139) lysW → / → valZ tRNA‑Lys/tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913780,9290CT98.3% 203.0 / ‑6.0 60intergenic (+11/‑139)lysW/valZtRNA‑Lys/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/31);  minor base G (1/0);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGA  >  NC_000913/780782‑781073
                                                                                                                                                   |                                                                                                                                                
gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGaa                                                                                                                                               <  2:720514/151‑1 (MQ=18)
gggAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGa                                                                                                                                                <  2:671479/150‑1 (MQ=18)
   agagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGAc                                                                                                                                            >  2:731738/1‑151 (MQ=25)
     aactccacccTTACAAGGAGGGGGTCGGCGATTCGAACCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAg                                                                                                                                          <  1:653902/144‑1 (MQ=255)
        aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCTCCCCCCACCGGGTCGTCAGCTCAGTTTTCCGAGCTGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATg                                                                                                                                       <  1:231406/151‑1 (MQ=14)
             ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAg                                                                                                                                  <  2:137387/151‑1 (MQ=255)
                 ccgaggcggGGGTCGGCGGTTCGAACCCGTCATCACCCAACACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGAt                                                                                                                                        <  1:683713/138‑1 (MQ=255)
                           gggaggcggTACGATCCCGTCATCACCCAACACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAAc                                                                                                                     <  1:599430/146‑1 (MQ=255)
                               ggcggTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGc                                                                                                                >  2:786550/1‑151 (MQ=255)
                               ggcggTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAGTTTTAAAATTGAAAACAGATGTTAAGCGTGTAGGATAACGtt                                                                                                                  >  2:799792/1‑149 (MQ=255)
                                      cGATCCCGTCATCACCCAAAACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCa                                                                                                            <  1:718883/148‑1 (MQ=255)
                                             gTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACg                                                                                                      <  2:400981/147‑1 (MQ=255)
                                               catcaCCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCg                                                                                                 >  1:317551/1‑150 (MQ=255)
                                                atcaCCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTTAACATTGAACATAGATGTGAAGCTTTACGAATAACGTTGCGCAAGCAACGGCACGt                                                                                                >  1:109606/1‑150 (MQ=11)
                                                atcaCCCAACACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACg                                                                                                      <  1:831603/144‑1 (MQ=255)
                                                acaaccaaccaGCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCg                                                                                                 <  1:94625/146‑1 (MQ=255)
                                                  cacccaccACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAgg                                                                                             <  2:847716/151‑1 (MQ=255)
                                                   acccaccACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAggg                                                                                            <  1:235287/151‑1 (MQ=255)
                                                   acccaccACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAggg                                                                                            >  2:199767/1‑151 (MQ=255)
                                                    cccaccACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATGGAACACAGAGGTTAATTGTTTCGGTTAACGTTGCGTCAGCAACGGCCAGGAggg                                                                                            >  1:312304/1‑150 (MQ=14)
                                                     ccaccaCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcg                                                                                          >  2:233453/1‑151 (MQ=255)
                                                       accaccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgag                                                                                        <  2:57111/151‑1 (MQ=255)
                                                         caccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGgcgagcg                                                                                      >  1:144401/1‑151 (MQ=255)
                                                           ccGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaa                                                                                    <  2:787397/151‑1 (MQ=255)
                                                             gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagc                                                                                  >  1:524526/1‑151 (MQ=255)
                                                              ggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagcg                                                                                 <  2:363574/151‑1 (MQ=255)
                                                               gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagcg                                                                                 >  1:389830/1‑150 (MQ=255)
                                                               gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGagcgaagcg                                                                                 >  1:108287/1‑150 (MQ=255)
                                                                   ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                             >  2:459340/1‑150 (MQ=255)
                                                                     aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                          >  2:811104/1‑151 (MQ=255)
                                                                         cAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                                        <  2:108310/149‑1 (MQ=255)
                                                                           gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                    <  1:199744/151‑1 (MQ=255)
                                                                           gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTTAACATTGAAGACGGATGATAAGCGGGTAGGATAACTTTGCGTCAGCAACGGCCGGTAGGGCAGGCGAAGCGAGTTATcc                                                                         >  1:3211/1‑146 (MQ=14)
                                                                                    gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                           <  1:311044/151‑1 (MQ=255)
                                                                                      aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACt                                                         >  2:348018/1‑151 (MQ=255)
                                                                                        ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTa                                                        >  2:450467/1‑150 (MQ=255)
                                                                                           aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGa                                                    <  2:245821/151‑1 (MQ=255)
                                                                                              tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACgg                                                 >  2:528544/1‑151 (MQ=255)
                                                                                                 aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTCGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTgg                                              >  2:129089/1‑151 (MQ=255)
                                                                                                        tGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCgg                                        >  2:357854/1‑150 (MQ=255)
                                                                                                         ggTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGt                                       <  2:771642/150‑1 (MQ=255)
                                                                                                          gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTGAAGGGGGGCTCGGTg                                      >  1:593073/1‑150 (MQ=14)
                                                                                                            cGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTATTAACGGGGGGTTCGGGGGa                                    >  1:41700/1‑150 (MQ=255)
                                                                                                               aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagta                                <  2:517911/151‑1 (MQ=255)
                                                                                                                 gTTCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAagtagt                              <  2:144424/151‑1 (MQ=255)
                                                                                                                  ttCGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGtt                             >  2:775405/1‑151 (MQ=255)
                                                                                                                    cGAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGttt                            <  2:437248/150‑1 (MQ=255)
                                                                                                                     gAATCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTgt                          >  2:666539/1‑151 (MQ=255)
                                                                                                                      aaTCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTgta                         <  2:273830/151‑1 (MQ=255)
                                                                                                                       aTCCTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGGGCGGAGGAAGTAGTTTgt                          >  1:235106/1‑149 (MQ=255)
                                                                                                                          cTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAccc                                                               >  2:403717/1‑109 (MQ=255)
                                                                                                                          cTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAccc                                                               <  1:403685/109‑1 (MQ=255)
                                                                                                                          cTGCACGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCATGACGGTTGGTTCGGAGGAATAAGCTTgtag                        >  1:35268/1‑148 (MQ=17)
                                                                                                                              aCGACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAg                 <  1:512846/151‑1 (MQ=255)
                                                                                                                                gACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgcg               >  2:469327/1‑151 (MQ=255)
                                                                                                                                gACCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgc                <  1:249918/150‑1 (MQ=255)
                                                                                                                                 aCCCACCAGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAgcgc              <  2:584640/151‑1 (MQ=255)
                                                                                                                                      ccaGTTTTAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTAt         <  1:551208/151‑1 (MQ=255)
                                                                                                                                             tAACATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGAGGAAAAAGTTTGTAGTAACCAGCCCAGTAGCGGGt    >  1:335801/1‑149 (MQ=21)
                                                                                                                                              aaCATTGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGa  <  1:375357/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGA  >  NC_000913/780782‑781073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: