Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	811,250	A→G	A290A (GCA→GCG)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	811,250	0	A	G	100.0%	153.0 / NA	48	A290A (GCA→GCG)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/25);  total (23/25)

CGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAA  >  NC_000913/811103‑811388                                                                                                                                                    |                                                                                                                                           cGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGCGATGCGCgg                                                                                                                                         >  1:551461/1‑151 (MQ=255)  ggggCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGgc                                                                                                                                        <  1:571846/151‑1 (MQ=255)             gCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCCTTCGCAGTGATGAGGGTGATTCGCGGCGCGaaaaa                                                                                                                                >  2:775530/1‑148 (MQ=255)                ttGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTgg                                                                                                                            <  2:810579/149‑1 (MQ=255)                ttGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTgg                                                                                                                            <  2:173710/149‑1 (MQ=255)                     ccAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCa                                                                                                                       <  1:78818/149‑1 (MQ=255)                     ccAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCAc                                                                                                                      <  1:627103/150‑1 (MQ=255)                        gTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCAt                                                                                                                  <  1:804641/151‑1 (MQ=255)                            ggTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTAcg                                                                                                              >  2:384194/1‑151 (MQ=255)                              tGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTAcgcg                                                                                                            >  2:461608/1‑151 (MQ=255)                                  ggATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGtttt                                                                                                        <  1:775474/151‑1 (MQ=255)                                    aTTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCg                                                                                                      <  1:705938/151‑1 (MQ=255)                                           gctgcAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCgg                                                                                                <  2:762169/150‑1 (MQ=255)                                              gcAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAg                                                                                              <  2:428846/149‑1 (MQ=255)                                                   tCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAgg                                                                                        <  2:364016/150‑1 (MQ=255)                                                    cgcccgccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAgg                                                                                        >  2:29130/1‑149 (MQ=255)                                                     gcccgccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCTCAGGGATGAGGATGATGCGCCGCGCGAACAACCGGCGGGCAACAATACGCGTTTTCGTGGCGGGGTACAGGa                                                                                       >  1:605956/1‑149 (MQ=255)                                                      cccgccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGAttt                                                                                    <  1:799379/151‑1 (MQ=255)                                                         gccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGcc                                                                                 >  2:697898/1‑151 (MQ=255)                                                          ccACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGGACAGGATTTGcc                                                                                 >  2:634188/1‑150 (MQ=255)                                                            aCCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCCGTGATGAGGGTGATGCGCGGCGCGAAAAAATTGCGGGCCTCATTTCGCCTTTTCGTGCCGGGGTACAGGATTTGGCGtt                                                                              >  1:104325/1‑151 (MQ=255)                                                             ccTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGttt                                                                             >  1:774789/1‑151 (MQ=255)                                                                tATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGttt                                                                             >  2:337096/1‑148 (MQ=255)                                                                tATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTAc                                                                           >  1:820857/1‑150 (MQ=255)                                                                 aTCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACg                                                                          <  2:186141/150‑1 (MQ=255)                                                                      cAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCt                                                                    <  1:641726/151‑1 (MQ=255)                                                                         cACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGAt                                                                 >  2:422194/1‑151 (MQ=255)                                                                           ccAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTc                                                               <  2:489193/151‑1 (MQ=255)                                                                                  gccgccCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAgc                                                        >  2:469986/1‑151 (MQ=255)                                                                                    cgcccgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAgcg                                                       >  1:255583/1‑150 (MQ=255)                                                                                      cccgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGcc                                                     >  2:689315/1‑150 (MQ=255)                                                                                       ccgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGCCAGTCGCAGTGATGAGGGGGATGTGCGGCGCGAAAAAATGGCGGAACTCATTACGCGTTTTCGCGCCGGAGTACAGGATTTTCCGTTTACGCTTGCTGATTCATGAAGCGACAt                                                   >  1:272160/1‑151 (MQ=255)                                                                                        cgcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATc                                                  <  2:639702/151‑1 (MQ=255)                                                                                         gcTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAACCTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATcc                                                 <  1:222033/151‑1 (MQ=255)                                                                                                      cATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGt                                    >  1:617998/1‑151 (MQ=255)                                                                                                      cATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGt                                    <  1:394627/151‑1 (MQ=255)                                                                                                         tACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCg                                  <  2:704398/150‑1 (MQ=255)                                                                                                          acctGCGTCGCTGGCGGTCATTCGGAGTGATGAGGGGGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCgg                                 >  1:411417/4‑150 (MQ=255)                                                                                                          aCGTGCGTCGCTGGCGGTCATTTTCGTTTCTGAGGGTGATGCGCGGCGGGAAAAACTGGCGGCGCTCATTTCGCGTTTTCGGTCCGGAGTACAGGATTTGCCGTTTAAGCTTGCTGATTCCTGCAGCGGCAACCAGCCATTGATTGTCgg                                 <  1:584931/150‑1 (MQ=255)                                                                                                                  cGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACa                         <  2:107618/150‑1 (MQ=255)                                                                                                                         ggTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCg                 >  1:519767/1‑151 (MQ=255)                                                                                                                         ggTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCg                 <  2:25547/151‑1 (MQ=255)                                                                                                                          gTCATTCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGt                <  1:697855/151‑1 (MQ=255)                                                                                                                              ttCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTAc             >  1:349196/1‑150 (MQ=255)                                                                                                                               tCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACa            <  2:533657/150‑1 (MQ=255)                                                                                                                               tCGCAGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACa            <  1:464991/150‑1 (MQ=255)                                                                                                                                   aGTGATGAGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTgg       >  1:652315/1‑151 (MQ=255)                                                                                                                                         gaGGGTGATGCGCGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGaa  >  2:394670/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                           CGGGGCAGCGGTGCTTTGCTCCAGTACGGTGGCGGATTATCTGCTGCAATTCGCCCGCCACCTTATCTACAGCACCAGTATGCCGCCCGCTCAGGCGCAGGCATTACGTGCGTCGCTGGCGGTCATTCGCAGTGATGAGGGTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAA  >  NC_000913/811103‑811388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑