| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 829,895 | A→G | Y26Y (TAT→TAC) | ybhG ← | putative membrane fusion protein (MFP) component of efflux pump, membrane anchor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 829,895 | 0 | A | G | 98.2% | 174.9 / NA | 55 | Y26Y (TAT→TAC) | ybhG | putative membrane fusion protein (MFP) component of efflux pump, membrane anchor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (28/26); minor base T (0/1); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGT > NC_000913/829753‑830041 | cGCCCAGCCCCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTaccacc < 1:270573/150‑1 (MQ=255) cccAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGt < 1:485263/151‑1 (MQ=255) ccAGCCCCTGGCCCGCTTTGATAGCATCCCCTTCGTCCGCCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATGTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAg < 1:503579/149‑1 (MQ=255) ggCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGc < 2:748643/151‑1 (MQ=255) cTTTGATGGCATCGCCTTCGTCCACCGCCAGCCATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCa < 1:534975/150‑1 (MQ=255) tttGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCAcg > 1:238187/1‑151 (MQ=255) aTAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCAcggcgg > 1:510542/1‑151 (MQ=255) aGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACggcggc > 1:744484/1‑150 (MQ=255) aTCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCaa < 1:664402/149‑1 (MQ=255) aTCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGt > 2:795071/1‑151 (MQ=255) cACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTAc > 2:705346/1‑151 (MQ=255) tCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACt > 1:502517/1‑150 (MQ=255) ccACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCg < 1:655881/150‑1 (MQ=255) gCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATc > 1:636168/1‑151 (MQ=255) ccAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATcc > 2:56664/1‑151 (MQ=255) cAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCg > 1:713635/1‑151 (MQ=255) cGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATc > 1:19902/1‑151 (MQ=255) aaCGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGAc > 2:398908/1‑150 (MQ=255) aaCGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACa < 2:67258/151‑1 (MQ=255) cgcCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTCCCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAg < 2:706829/148‑1 (MQ=255) ccccccAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGtttt > 2:446915/1‑151 (MQ=255) acGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACt > 2:183071/1‑151 (MQ=255) acGAAAACTAAGATTTCCCGTAGTAAAACCCCCGTTCCCAACCAGCGTCAGGCCTTTATCCTGGCGTTTTTGGTACCACCGGTGGCCTCCGGCAACCCCGGCGGCAAGTACCACTCCCGCCAATCCGATCACGACAGGTTTTTTCATCAc < 1:771397/150‑1 (MQ=255) cGGAAACTAAGATTTACCGTACGAATACCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTCCCACCAGTAGCCTCCGGCAACCCCGGCGGCAAGTCCCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACt < 1:129272/150‑1 (MQ=255) ggAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTAc > 2:454196/1‑151 (MQ=255) cTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACt > 1:335272/1‑151 (MQ=255) gATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCtt > 1:52732/1‑151 (MQ=255) gATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCt < 2:792083/150‑1 (MQ=255) ccGTACGAATATCCACGTTGCCATACAGCGTCAGGCCTTTATCCTGGCGGCTTGGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCg < 1:425669/149‑1 (MQ=255) tACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCTCCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAAt > 1:771071/1‑151 (MQ=255) tACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAAt > 1:331186/1‑151 (MQ=255) ctaaTATCCCCGTTGCCATACACCGTCAGGCCGTTATCCTGGGGGCTTTGGTCCCCCCAGTACCCTCCGGCAACCACGGCGGCAAGTCCCCCTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAAt < 2:373532/147‑1 (MQ=255) aaTATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCtt > 2:150809/1‑151 (MQ=255) atatCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTg > 1:306339/1‑151 (MQ=255) tatCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTg < 2:124219/150‑1 (MQ=255) tatCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGc > 2:451264/1‑151 (MQ=255) acccccGTTCCCATCCAGCGCCAGGCCGTTTTCCTGGCGGGTTTGGTCCCACCAGTAGCCTCCGGCACCCACGGCGGCAAGTACCTCTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTg < 2:383497/144‑1 (MQ=255) ccACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCaa > 1:218460/1‑150 (MQ=255) cACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCaaa > 2:324060/1‑150 (MQ=255) cGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATc < 1:748920/151‑1 (MQ=255) ccATACAGCGTCAGGCCTTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCCCTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTc < 2:218484/151‑1 (MQ=255) gCGTCAGGCCGTTATCCTGGCGGCTTTGGTCCCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGa < 2:263683/151‑1 (MQ=255) cGTCAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGAc > 1:360503/1‑151 (MQ=255) cAGGCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAgg > 2:705420/1‑151 (MQ=255) ggCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCGCCGGCAACCACGGCGGCAAGTCCCACTTCCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt < 2:318317/150‑1 (MQ=255) gCCGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTc > 2:73073/1‑150 (MQ=255) cGTTATCCTGGCGGCTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCAcc > 2:223267/1‑151 (MQ=255) ttATCTCGGCGGCTTTGTTCTCACCAGAAGCCTCCGGAATCCCCGGCTGCAAGTCCCTCTCCCGCCAATCCGATCACGATAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCTATATGACAGGTCAcct < 1:826728/150‑2 (MQ=255) ccgggcggcTTTGGTACCCCCAGTAGCCCCCGGCAACCGCGGGGGAAAGTACCACTCCCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTc < 1:250117/145‑1 (MQ=255) ggcggcTTTGGTACCCCCAGTAGCCTCCGGCAACCCCGGCGGCAAGTACCCCTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGtt < 1:9376/150‑1 (MQ=255) ggcTTTGGTCCCACCAGTAGCCCCCGGCACCCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGAt < 1:612960/150‑1 (MQ=255) ggcTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGAt < 2:177891/150‑1 (MQ=255) ggcTTTGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATc > 2:716269/1‑151 (MQ=255) tttGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCa < 1:394074/149‑1 (MQ=255) ttGGTACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGt < 2:636210/150‑1 (MQ=255) | CGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGAAACTAAGATTTACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGT > NC_000913/829753‑830041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |