Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 832,662 T→C M97V (ATG→GTG)  ybiA ← DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,6620TC97.9% 153.3 / ‑4.2 47M97V (ATG→GTG) ybiADUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (19/27);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

AGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTT  >  NC_000913/832517‑832808
                                                                                                                                                 |                                                                                                                                                  
aGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCt                                                                                                                                               >  2:856792/1‑151 (MQ=255)
 gTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTg                                                                                                                                              >  2:785673/1‑151 (MQ=255)
   cGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGtt                                                                                                                                            <  1:22606/151‑1 (MQ=255)
   cGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGtt                                                                                                                                            >  1:854288/1‑151 (MQ=255)
          tGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCttt                                                                                                                                        <  1:14584/148‑1 (MQ=255)
          tGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGGTTTGCGGGCGCGGGTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGa                                                                                                                                      >  2:857623/1‑150 (MQ=255)
           gCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGAc                                                                                                                                     <  1:568586/150‑1 (MQ=255)
                 aCCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTc                                                                                                                               <  2:721807/150‑1 (MQ=255)
                   cATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCa                                                                                                                            <  1:718622/151‑1 (MQ=255)
                      gACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGt                                                                                                                          <  2:419394/150‑1 (MQ=255)
                      gACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGGTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGtt                                                                                                                         >  2:560355/1‑151 (MQ=255)
                              tctcCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACg                                                                                                                   >  1:657117/1‑149 (MQ=255)
                                        aaGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGa                                                                                                       <  1:823258/151‑1 (MQ=255)
                                              cGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATc                                                                                                 <  2:396906/151‑1 (MQ=255)
                                                    ccGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGccc                                                                                           >  2:546455/1‑151 (MQ=255)
                                                      gTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGcccc                                                                                          >  2:757038/1‑150 (MQ=255)
                                                        aTGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtg                                                                                       >  1:253747/1‑151 (MQ=255)
                                                         tGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgc                                                                                      >  1:607125/1‑151 (MQ=255)
                                                          gCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgc                                                                                      <  2:667565/150‑1 (MQ=255)
                                                             cAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgcgt                                                                                    <  2:233722/149‑1 (MQ=255)
                                                              aaCCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCAtgcgtgcg                                                                                 <  2:147910/151‑1 (MQ=255)
                                                                   gTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGAc                                                                            <  1:28166/151‑1 (MQ=255)
                                                                          gggCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAggg                                                                     <  2:148913/151‑1 (MQ=255)
                                                                             cgcgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGgaa                                                                  <  1:296044/151‑1 (MQ=255)
                                                                             cgcgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGgaa                                                                  <  2:695897/151‑1 (MQ=255)
                                                                              gcgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGga                                                                   >  1:166307/1‑149 (MQ=255)
                                                                               cgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGgaaga                                                                <  2:20555/151‑1 (MQ=255)
                                                                               cgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGgaaga                                                                <  2:253772/151‑1 (MQ=255)
                                                                               cgGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGga                                                                   >  2:400639/1‑148 (MQ=255)
                                                                                     ccAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCg                                                          <  1:653276/151‑1 (MQ=255)
                                                                                                   cgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTcgc                                            <  1:474440/151‑1 (MQ=255)
                                                                                                   cgcaGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTcgc                                            <  1:460242/151‑1 (MQ=255)
                                                                                                      aGTTCTGCATGCTGTACGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCCTGCGTGCGGCGAGCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGt                                         >  1:352013/1‑151 (MQ=255)
                                                                                                       gTTCTTCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGt                                         >  1:171216/1‑150 (MQ=255)
                                                                                                            gCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTAttttt                                   >  1:40513/1‑151 (MQ=255)
                                                                                                            gCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTAtt                                      <  2:460946/148‑1 (MQ=255)
                                                                                                            gCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTAtt                                      <  1:577757/148‑1 (MQ=255)
                                                                                                                gCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcatc                               <  2:523050/151‑1 (MQ=255)
                                                                                                                   gTTCGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTtcatca                              <  1:285453/149‑1 (MQ=255)
                                                                                                                      cGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGa                          >  2:747736/1‑150 (MQ=255)
                                                                                                                      cGAATTTAGCGCGAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGa                          >  2:54921/1‑150 (MQ=255)
                                                                                                                           ttAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAAttt                      <  2:762810/149‑1 (MQ=255)
                                                                                                                                 cgAAGTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAAAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCt              <  2:628112/151‑1 (MQ=255)
                                                                                                                                     gTGCTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGaaa          <  1:54832/151‑1 (MQ=255)
                                                                                                                                        cTTTTCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGAAGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt        >  2:829810/1‑150 (MQ=255)
                                                                                                                                         ttttCGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAAt        >  1:335690/1‑149 (MQ=255)
                                                                                                                                             cGCACCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGtt  <  2:806119/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGTCGATTCTTGCCCTTACCATGACCACCGTCTCCCCAGTAAGCATCGTTTTCCGTATGCTCAACCAGTTTTGCGGGCGCGGTTGCCAGCAAGAGCGCACGCAGTTCTGCATGCTGTTCGAATTTAGCGCGAAGTGCTTTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTT  >  NC_000913/832517‑832808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑