Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	846,072	T→C	H131R (CAT→CGT)	glnQ ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	846,072	0	T	C	100.0%	172.8 / NA	49	H131R (CAT→CGT)	glnQ	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

TGCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGA  >  NC_000913/845925‑846216                                                                                                                                                    |                                                                                                                                                 tGCATAACCTTCAGCACTTCATGTCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGAcgtg                                                                                                                                               >  2:639362/1‑151 (MQ=255)  gCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGAcgtg                                                                                                                                               <  2:674179/150‑1 (MQ=255)  gCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGAcgt                                                                                                                                                <  2:604167/149‑1 (MQ=255)  gCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGAcgt                                                                                                                                                <  1:5068/149‑1 (MQ=255)      aaCCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGt                                                                                                                                          <  2:670039/151‑1 (MQ=255)       aCCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGtt                                                                                                                                         >  2:809929/1‑151 (MQ=255)         cTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCt                                                                                                                                       >  1:66018/1‑151 (MQ=255)          ttCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTg                                                                                                                                      >  2:281582/1‑151 (MQ=255)           tCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTg                                                                                                                                      <  1:79750/150‑1 (MQ=255)            cAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGTAAGGGTAGTGACGTGCACGTTCTGc                                                                                                                                     <  2:722197/150‑1 (MQ=255)             aGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCa                                                                                                                                   <  2:530159/151‑1 (MQ=255)                   tCATGGCGCATTTCCGGGTCAAGGGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGGcc                                                                                                                               <  1:639681/149‑1 (MQ=255)                      tGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACtt                                                                                                                          >  1:518876/1‑151 (MQ=255)                        gcgcAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTc                                                                                                                        <  1:281557/151‑1 (MQ=255)                         cgcAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCg                                                                                                                       >  2:343072/1‑151 (MQ=255)                           cAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGcc                                                                                                                     >  2:716434/1‑151 (MQ=255)                                ccGGGACACGATCGGAGGTCGCTTCATCAACCAGCATCATTTACGGCTTCCCCGCCAGCGCGCGGGCAAGCGCCACACGCTGCTGTTGACCACCAGAAACTTCGGAAGGGTAGTGACGTGCACGTGCTGCCAGACCGACTTTCGCcat                                                                                                                   <  1:251441/148‑2 (MQ=255)                                          agCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGcc                                                                                                      >  1:220374/1‑151 (MQ=255)                                              gAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGtt                                                                                                  >  1:778340/1‑151 (MQ=255)                                                 gTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGttttt                                                                                               <  1:230436/151‑1 (MQ=255)                                                            aaaCAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCtcttct                                                                                    >  1:572566/1‑151 (MQ=255)                                                              aCAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCttt                                                                                  <  1:373715/151‑1 (MQ=255)                                                                aGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGt                                                                                <  2:178475/151‑1 (MQ=255)                                                                  catcatTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGAATTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTc                                                                              >  2:198683/1‑151 (MQ=255)                                                                       ttttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGcc                                                                         >  2:417682/1‑151 (MQ=255)                                                                         ttCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCca                                                                        <  1:778100/150‑1 (MQ=255)                                                                           cGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcacg                                                                      <  2:361452/150‑1 (MQ=255)                                                                             gCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCcacgcac                                                                   <  1:343049/151‑1 (MQ=255)                                                                                  aCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCttt                                                                                  <  1:806577/131‑1 (MQ=255)                                                                                  aCCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCttt                                                                                  >  2:806633/1‑131 (MQ=255)                                                                                      ccAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCgg                                                          <  2:555698/151‑1 (MQ=255)                                                                                                      cGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGttt                                          >  2:576410/1‑151 (MQ=255)                                                                                                         cacaCGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTcc                                       >  2:381083/1‑151 (MQ=255)                                                                                                           cacGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAg                                     <  2:81896/151‑1 (MQ=255)                                                                                                            acGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAgc                                    >  1:790581/1‑151 (MQ=255)                                                                                                              gctgctGTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAgcgc                                  <  1:330227/151‑1 (MQ=255)                                                                                                                 gctgTTGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCt                                 <  2:591236/149‑1 (MQ=255)                                                                                                                     ttGACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCGCGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAg                            <  1:183028/150‑1 (MQ=255)                                                                                                                       gACCACCAGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATg                         <  2:204367/151‑1 (MQ=255)                                                                                                                            ccaGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCggg                     >  1:331534/1‑150 (MQ=255)                                                                                                                             caGAAAGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGaa                   <  2:408963/151‑1 (MQ=255)                                                                                                                                aaaGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGGAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAaga                 <  1:810850/150‑1 (MQ=255)                                                                                                                                aaaGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACACACGCGTAGCGGGCCAAACATGACg                                             >  2:147165/1‑122 (MQ=255)                                                                                                                                aaaGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACACACGCGTAGCGGGCCAAACATGACg                                             <  1:147142/122‑1 (MQ=255)                                                                                                                                 aaGTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGAGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAgg               >  1:831087/1‑151 (MQ=255)                                                                                                                                   gTTCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTa             <  2:284375/151‑1 (MQ=255)                                                                                                                                    cgcGGAAGGACAGTGACGTGCACGTTCTGACAGACCGCCTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGGAGCGGGCCAAACATGACGTTTTCCAACGCTGTCAGATGCGGGACGAGGTa             >  1:640728/3‑150 (MQ=255)                                                                                                                                     tCGGAAGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTaa            <  2:801926/150‑1 (MQ=255)                                                                                                                                         aaGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActg       <  1:526625/151‑1 (MQ=255)                                                                                                                                          aGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActgc      >  2:557390/1‑151 (MQ=255)                                                                                                                                          aGGGTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActgc      <  1:833893/151‑1 (MQ=255)                                                                                                                                            ggTAGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActgctg    >  2:581169/1‑151 (MQ=255)                                                                                                                                               aGTGACGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGa  <  2:132651/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TGCATAACCTTCAGCACTTCATGGCGCAGTTCCGGGTCAAGAGCGGAAGTCGGTTCATCAAACAGCATCATTTTCGGCTTCACCGCCAGCGCGCGGGCAATCGCCACACGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGA  >  NC_000913/845925‑846216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑