Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	904,502	T→C	intergenic (‑35/‑91)	ybjP ← / → ybjQ	lipoprotein/UPF0145 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	904,502	0	T	C	100.0%	206.0 / NA	57	intergenic (‑35/‑91)	ybjP/ybjQ	lipoprotein/UPF0145 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/29);  total (28/29)

CGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGTTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGG  >  NC_000913/904359‑904648                                                                                                                                                |                                                                                                                                                   cGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGa                                                                                                                                             <  2:531414/151‑1 (MQ=255)      ggACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGataat                                                                                                                                         >  1:550247/1‑150 (MQ=255)          cACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATg                                                                                                                                     >  1:150820/1‑150 (MQ=255)                gtgtGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCt                                                                                                                              >  1:772646/1‑151 (MQ=255)                    gCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCa                                                                                                                          <  2:573097/151‑1 (MQ=255)                     ccGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCa                                                                                                                          >  2:853048/1‑150 (MQ=255)                      cGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCaat                                                                                                                        <  2:261945/151‑1 (MQ=255)                       gTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCaat                                                                                                                        >  1:207939/1‑150 (MQ=255)                            cTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGc                                                                                                                  >  2:309184/1‑151 (MQ=255)                              ttATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAataata                                                                                                                    <  2:168520/147‑1 (MQ=255)                              ttATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCg                                                                                                                 <  1:357356/150‑1 (MQ=255)                               tataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGt                                                                                                                <  2:807273/150‑1 (MQ=255)                                ataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTAt                                                                                                              >  2:682505/1‑151 (MQ=255)                                ataAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTGCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTa                                                                                                               <  1:131019/150‑1 (MQ=255)                                       ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTc                                                                                                         <  1:780281/149‑1 (MQ=255)                                       ggAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCt                                                                                                        <  1:186592/150‑1 (MQ=255)                                         aGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGAt                                                                                                     >  2:321669/1‑151 (MQ=255)                                            gACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATaa                                                                                                   <  1:856078/150‑1 (MQ=255)                                             aCTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATg                                                                                                 <  2:728829/151‑1 (MQ=255)                                                gtggtgCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGc                                                                                                <  1:213444/149‑1 (MQ=255)                                                              gaggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAgg                                                                                 <  1:357274/150‑1 (MQ=255)                                                              gaggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAgg                                                                                 <  1:699434/150‑1 (MQ=255)                                                               aggagCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCt                                                                               <  1:723856/151‑1 (MQ=255)                                                                  agCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAg                                                                            <  2:835765/151‑1 (MQ=255)                                                                     aGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCaa                                                                         <  2:273587/151‑1 (MQ=255)                                                                        gcgcAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAAc                                                                       <  2:617842/150‑1 (MQ=255)                                                                                       aTTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATg                                                       >  2:840358/1‑151 (MQ=255)                                                                                            cAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtt                                                  >  2:442207/1‑151 (MQ=255)                                                                                            cAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtt                                                  >  1:176922/1‑151 (MQ=255)                                                                                            cAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAt                                                   <  1:246822/150‑1 (MQ=255)                                                                                             aaTTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAttt                                                 >  2:382201/1‑151 (MQ=255)                                                                                              aTTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAtttt                                                >  2:649122/1‑151 (MQ=255)                                                                                                 tGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCt                                              <  2:198511/150‑1 (MQ=255)                                                                                                          gcATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCa                                     >  2:584843/1‑150 (MQ=255)                                                                                                           cATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAc                                   <  1:309160/151‑1 (MQ=255)                                                                                                           aaTAATGATTTCCTTATAAGCGATCGCTCTGAAAGAGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTATGAGATCGAAGGCTTAGCAAACAAGGCGATCGATCATGCAATTTTCTACAACCCCaa                                    >  1:571463/2‑150 (MQ=255)                                                                                                              aaTGATTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAActct                                >  2:574255/1‑151 (MQ=255)                                                                                                                  aTTTCCTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGaa                            <  2:586979/151‑1 (MQ=255)                                                                                                                      ccTTATAAGCGATTGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGc                         <  2:749482/150‑1 (MQ=255)                                                                                                                      ccTTATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                        >  1:203692/1‑151 (MQ=255)                                                                                                                        ttATAAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAGTGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                        >  1:533556/1‑149 (MQ=255)                                                                                                                         tataAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGcc                        >  1:270949/1‑148 (MQ=255)                                                                                                                           taAGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc                   >  1:793007/1‑151 (MQ=255)                                                                                                                            aaGCGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc                   <  1:303965/150‑1 (MQ=255)                                                                                                                               cGATCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCg               <  2:486695/151‑1 (MQ=255)                                                                                                                                 aTCGCTCTGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGtt             >  2:495247/1‑151 (MQ=255)                                                                                                                                     ctctGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAat         >  2:641550/1‑151 (MQ=255)                                                                                                                                      tctGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAat         >  1:663940/1‑150 (MQ=255)                                                                                                                                        tGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAatat       >  1:34029/1‑150 (MQ=255)                                                                                                                                        tGAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATAtt      >  1:62521/1‑151 (MQ=255)                                                                                                                                         gAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAt                                                   >  2:118351/1‑105 (MQ=255)                                                                                                                                         gAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAAt                                                   <  1:118328/105‑1 (MQ=255)                                                                                                                                         gAAAGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATAtt      <  2:575542/150‑1 (MQ=255)                                                                                                                                           aaGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCg   >  2:366388/1‑151 (MQ=255)                                                                                                                                           aaGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCg   <  1:736670/151‑1 (MQ=255)                                                                                                                                            aGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCgg  >  1:764409/1‑151 (MQ=255)                                                                                                                                            aGCGCTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCgg  <  1:663598/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CGCAAGGACCACTGCGTGTGCCGTTATCTTTATAAGCTGGAGTGACTGTGGTGCAGGCACTGAGGAGCAGTGCGCAGGGGATAAGCATTGTCAATTTGCTGTAGCGCATAATGATTTCCTTATAAGCGATCGCTCTGAAAGCGTTCTACGATAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGG  >  NC_000913/904359‑904648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑