Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	964,931	A→G	E204E (GAA→GAG)	ycaI →	ComEC family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	964,931	0	A	G	100.0%	178.7 / NA	53	E204E (GAA→GAG)	ycaI	ComEC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

CCGCTCACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAACGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCTGCCTGGGCG  >  NC_000913/964794‑965077                                                                                                                                          |                                                                                                                                                   ccGCTCACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTcc                                                                                                                                       <  2:395751/151‑1 (MQ=255)   gCTCACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTcc                                                                                                                                       <  1:237732/149‑1 (MQ=255)   gCTCACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTcc                                                                                                                                       >  2:237756/1‑149 (MQ=255)    cTCACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTcccc                                                                                                                                     >  1:87623/1‑150 (MQ=255)       aCCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCaa                                                                                                                                   >  1:328740/1‑149 (MQ=255)       aCCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAg                                                                                                                                 <  2:725096/151‑1 (MQ=255)        ccGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGa                                                                                                                                <  2:281116/151‑1 (MQ=255)            ccGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATc                                                                                                                            >  1:816815/1‑151 (MQ=255)            ccGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATc                                                                                                                            <  2:154326/151‑1 (MQ=255)            ccGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATc                                                                                                                            >  1:406968/1‑151 (MQ=255)                tttCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCaaa                                                                                                                         <  2:647837/150‑1 (MQ=255)                     gCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAatat                                                                                                                    <  1:135239/150‑1 (MQ=255)                       aGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCAt                                                                                                                 >  2:343108/1‑151 (MQ=255)                          cAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATgcg                                                                                                              <  1:719174/151‑1 (MQ=255)                           aaGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATgcgc                                                                                                             >  2:575706/1‑151 (MQ=255)                                 cATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACt                                                                                                       >  1:361796/1‑151 (MQ=255)                                  aTTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTg                                                                                                      >  1:165539/1‑151 (MQ=255)                                    tGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTgg                                                                                                     <  1:559935/150‑1 (MQ=255)                                     gAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGa                                                                                                    >  1:158239/1‑150 (MQ=255)                                      aaCCGAATTGTAGCCTGCTTGCACATTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAAc                                                                                                  <  1:767689/151‑1 (MQ=255)                                      aaCCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAAc                                                                                                  <  1:743934/151‑1 (MQ=255)                                         cGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGgc                                                                                               >  1:145427/1‑151 (MQ=255)                                         cGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGgc                                                                                               >  1:806579/1‑151 (MQ=255)                                             ttGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCAt                                                                                           >  1:532673/1‑151 (MQ=255)                                              tGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCAt                                                                                           <  2:238642/150‑1 (MQ=255)                                                 aGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTa                                                                                        >  2:458690/1‑150 (MQ=255)                                                 aGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTa                                                                                        <  1:771535/150‑1 (MQ=255)                                                   ccTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATg                                                                                     <  1:203594/151‑1 (MQ=255)                                                      gCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATgg                                                                                    <  2:309135/149‑1 (MQ=255)                                                        gTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGat                                                                                <  2:618314/151‑1 (MQ=255)                                                                     ggCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGAtt                                                                       <  1:529797/147‑1 (MQ=255)                                                                       cGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACAt                                                                 >  2:539319/1‑151 (MQ=255)                                                                          cACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGc                                                              >  2:497525/1‑151 (MQ=255)                                                                            cTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCtt                                                            >  2:440092/1‑151 (MQ=255)                                                                             tACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCttt                                                           >  2:770739/1‑151 (MQ=255)                                                                                      cGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGtt                                                  <  1:702781/151‑1 (MQ=255)                                                                                       gCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTg                                                 >  2:595503/1‑151 (MQ=255)                                                                                              cccTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTgctggctg                                          <  1:854835/151‑1 (MQ=255)                                                                                               ccTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCt                                                             >  1:859322/1‑131 (MQ=255)                                                                                               ccTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCt                                                             <  2:859381/131‑1 (MQ=255)                                                                                                        ggAATGCGGTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCAt                                >  1:248888/1‑151 (MQ=255)                                                                                                               ggTTATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGt                         >  2:667956/1‑151 (MQ=255)                                                                                                                gTTATTCTTGG‑TTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGACATCACAATTATCATGCGCGAGACGGGGACGGCGAATTTAATGGCGATATCGGGATGGCACATCCCTTGTGCGGCGTTGCTGGCTGCCGGGCTCATTCACAGTg                        >  1:575313/1‑150 (MQ=255)                                                                                                                  tATTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTgg                       <  2:675742/150‑1 (MQ=255)                                                                                                                   aTTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGa                      <  1:278272/150‑1 (MQ=255)                                                                                                                   aTTCTTGGTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGAc                     >  1:780124/1‑151 (MQ=255)                                                                                                                          gTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAAtttt              <  2:106143/151‑1 (MQ=255)                                                                                                                          gTTTAGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAAttt               <  1:429915/150‑1 (MQ=255)                                                                                                                              aGGTATGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAATGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCTg          <  1:853430/151‑1 (MQ=255)                                                                                                                                  aTGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCTGCCt       <  2:853852/150‑1 (MQ=255)                                                                                                                                  aTGGGGGAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCTGCCTg      >  2:158698/1‑151 (MQ=255)                                                                                                                                  aTGGAGGAGCGGTTACCCGTCCCAAACGAAATCAAAAATATCATGCGCGAGACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTCCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCt           >  1:858601/1‑146 (MQ=255)                                                                                                                                      gggAGCGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCTGCCTGGgcg  <  1:440058/151‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                   CCGCTCACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAACGCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAACGGTTATCCGTCCCCAAAGAAATCAAAAATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCCGGACTCATTCGCAGTGGACAAATTTTTCTGCCTGGGCG  >  NC_000913/964794‑965077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑