Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,078	T→C	I238T (ATT→ACT)	lpxK →	lipid A 4'kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,078	0	T	C	100.0%	109.7 / NA	37	I238T (ATT→ACT)	lpxK	lipid A 4'kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/18);  total (19/18)

GCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTG  >  NC_000913/968942‑969221                                                                                                                                         |                                                                                                                                                gCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATccgcc                                                                                                                                     >  1:100926/1‑149 (MQ=255)   ggTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTCCCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCAGCCGCcgcg                                                                                                                                  <  1:763516/150‑1 (MQ=255)     tAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGGCTGGGCCTCCGCCGCGCtt                                                                                                                               >  2:706806/1‑151 (MQ=255)              aCGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCAc                                                                                                                      <  2:53118/151‑1 (MQ=255)                ggcggTGTCCCTCGCCGCGGTGAAAGCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGc                                                                                                                    <  1:839808/151‑1 (MQ=255)                   ggTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTg                                                                                                                  <  1:100945/150‑1 (MQ=255)                    gtgtCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGa                                                                                                                 >  2:292808/1‑150 (MQ=255)                     tgtCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGa                                                                                                                 >  2:624911/1‑149 (MQ=255)                      gtCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGa                                                                                                                 >  2:568023/1‑148 (MQ=255)                          cTCGCCGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACCTGTAGTGGCCATCGCGGGGACTGGGCATGCGCCGGGCTTTTTTGCCAAGCTGGAGGtgcg                                                                                                          >  1:455791/1‑149 (MQ=255)                             gCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTgg                                                                                                       >  2:431604/1‑151 (MQ=255)                                           cccATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGaa                                                                                          >  1:623083/1‑150 (MQ=255)                                            ccATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGaaaa                                                                                        <  1:783887/151‑1 (MQ=255)                                                gCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgt                                                                                    <  1:484514/151‑1 (MQ=255)                                                 cATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgtg                                                                                   >  2:392885/1‑151 (MQ=255)                                                  aTCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAAtgtg                                                                                   >  1:217496/1‑150 (MQ=255)                                                          ccGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTgg                                                                          >  1:424776/1‑151 (MQ=255)                                                                  ggCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGatcat                                                                  <  2:212782/151‑1 (MQ=255)                                                                  ggCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGatcat                                                                  <  2:73648/151‑1 (MQ=255)                                                                              aCGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAAc                                                      <  2:193058/151‑1 (MQ=255)                                                                                  aCCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATg                                                  >  1:610316/1‑151 (MQ=255)                                                                                       tACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCgg                                               >  1:487088/1‑149 (MQ=255)                                                                                         cgcgTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGt                                           >  1:331641/1‑151 (MQ=255)                                                                                               gtgACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTAGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGc                                     <  1:360929/151‑1 (MQ=255)                                                                                                 gACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCg                                    <  2:445487/150‑1 (MQ=255)                                                                                                        cTCAGCTTGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTa                              >  2:439907/1‑149 (MQ=255)                                                                                                               tGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAGCCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCgg                      <  1:273985/150‑1 (MQ=255)                                                                                                               tGAACATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCggg                     >  2:313538/1‑151 (MQ=255)                                                                                                                 aaCATGTAGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCa                   <  2:456686/151‑1 (MQ=255)                                                                                                                   cATGTAGTGGTGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCggg                     <  1:257206/147‑1 (MQ=255)                                                                                                                        aGTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTg            <  2:414789/151‑1 (MQ=255)                                                                                                                         gTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTgg           <  2:534606/151‑1 (MQ=255)                                                                                                                         gTGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTgg           >  1:737039/1‑151 (MQ=255)                                                                                                                          tGGCGATGGCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGCGGTGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGCAAGCGCCGGGCAAAc                >  1:774551/1‑145 (MQ=255)                                                                                                                              gATGGCGGGTACTGGGCATCCGCCGCGCTTTTTTGCCATGCTGAAGATGTGTGGCGTCCAACCGGAAAAATGTGTACCGCTTGCCGATCATCAGTATTTGAACCATGCGGATGTCAGTTCGTTGGTAATCGCCGGGCAAACTCTgtt          <  1:757499/147‑3 (MQ=255)                                                                                                                                 ggCGGGGACTGGGCATCCGCCGCGCTTTTTTACCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACt   >  1:588300/1‑151 (MQ=255)                                                                                                                                  gCGGGGACTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTg  <  1:727008/151‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                GCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTG  >  NC_000913/968942‑969221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑