Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	982,139	C→T	R365* (CGA→TGA)	ldtD →	murein L,D‑transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	982,139	0	C	T	100.0%	186.3 / NA	51	R365* (CGA→TGA)	ldtD	murein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/30);  total (21/30)

CTACGATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCGATGATGAG  >  NC_000913/981994‑982284                                                                                                                                                  |                                                                                                                                                  cTACGATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTg                                                                                                                                              >  1:830681/1‑151 (MQ=255)     gATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCt                                                                                                                                          <  1:186939/151‑1 (MQ=255)        aaTGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTtg                                                                                                                                        <  2:529991/150‑1 (MQ=255)         aTGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTtg                                                                                                                                        <  1:28580/149‑1 (MQ=255)           gAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTtgctgc                                                                                                                                    <  2:617077/151‑1 (MQ=255)            aaCTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGcc                                                                                                                                   >  1:50877/1‑151 (MQ=255)             aCTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCa                                                                                                                                  <  1:732695/151‑1 (MQ=255)                     aaGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCt                                                                                                                          <  1:663227/151‑1 (MQ=255)                      aGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCtt                                                                                                                         <  1:727419/151‑1 (MQ=255)                       gCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCttt                                                                                                                        <  2:434180/151‑1 (MQ=255)                             aaaCGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCg                                                                                                                  >  2:832719/1‑151 (MQ=255)                             aaaCGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCg                                                                                                                  <  2:389595/151‑1 (MQ=255)                                cGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGa                                                                                                               <  2:854721/151‑1 (MQ=255)                                 gTTTTCAGGGATGGCAAGGATTGGAGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGTGa                                                                                                               <  1:692249/150‑1 (MQ=255)                                 gTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTCGCTGCCAACAGAGCTTTCTACCGGGAt                                                                                                              <  2:176412/151‑1 (MQ=255)                                   tttCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGAt                                                                                                              >  1:541842/1‑149 (MQ=255)                                   tttCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATc                                                                                                             <  1:365774/150‑1 (MQ=255)                                    ttCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCa                                                                                                            <  2:424016/150‑1 (MQ=255)                                     tCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCAt                                                                                                           <  1:340635/150‑1 (MQ=255)                                       aGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGt                                                                                                        >  2:172856/1‑151 (MQ=255)                                           aTGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAAc                                                                                                    >  1:359692/1‑151 (MQ=255)                                                aaGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTcc                                                                                               <  1:565787/151‑1 (MQ=255)                                                aaGGATTCGGGGCAGATGGTGCTATTGTCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTcc                                                                                               >  1:182313/1‑151 (MQ=255)                                                 aGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCg                                                                                              <  2:220149/151‑1 (MQ=255)                                                  ggATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCgg                                                                                             >  2:803120/1‑151 (MQ=255)                                                      tGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGCGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCt                                                                                          >  2:754700/1‑150 (MQ=255)                                                       gggggCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTGCTACCGGGATCATGGTTAACATTCCGGCCTa                                                                                         <  1:316022/150‑1 (MQ=255)                                                         gggCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCT‑GTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCg                                                                                     >  2:687382/1‑151 (MQ=255)                                                          ggCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCg                                                                                     <  2:483526/151‑1 (MQ=255)                                                                         tGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATc                                                                        >  2:701735/1‑149 (MQ=255)                                                                            cccGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAAc                                                                   >  2:221271/1‑151 (MQ=255)                                                                             ccGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACg                                                                  >  2:455708/1‑151 (MQ=255)                                                                             ccGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACg                                                                  >  1:29896/1‑151 (MQ=255)                                                                                gCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGc                                                                >  2:536119/1‑150 (MQ=255)                                                                                     acGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATc                                                            >  1:144342/1‑149 (MQ=255)                                                                                     acGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAg                                                          <  2:305061/151‑1 (MQ=255)                                                                                          aCTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGc                                                      >  1:85314/1‑150 (MQ=255)                                                                                           cTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTg                                                    >  1:625242/1‑151 (MQ=255)                                                                                             ggTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGa                                                  <  1:2400/151‑1 (MQ=255)                                                                                               ttAAACGTAACGACCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCATCAGAGCTTTCTCCCGGGATCATGGTTACCATTCCGGCCTATTCGCTGATCTACTATCAGAACGGCAATCAGGTg                                                       <  1:514240/144‑1 (MQ=255)                                                                                               ttAAAAGTAACGCCCGAACAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGAtt                                                >  1:106683/1‑151 (MQ=255)                                                                                                    cGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTcgcg                                            >  1:93158/1‑150 (MQ=255)                                                                                                       aaCGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTc                                        <  2:833424/151‑1 (MQ=255)                                                                                                                  aGCGTGCTGGTGTGTTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTgtcggtcg                             <  2:859452/151‑1 (MQ=255)                                                                                                                           gtgtgtTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCg                    <  1:475570/151‑1 (MQ=255)                                                                                                                             gtgtTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGc                   <  1:651907/150‑1 (MQ=255)                                                                                                                             gtgtTGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGc                   <  2:721876/150‑1 (MQ=255)                                                                                                                                 tGGCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAAc              >  2:781193/1‑151 (MQ=255)                                                                                                                                   gCTCTCAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGc            <  1:174225/151‑1 (MQ=255)                                                                                                                                        cAACATCCAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCgat        <  1:86779/150‑1 (MQ=255)                                                                                                                                              ccAGTGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCGATGATGAg  <  1:473284/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CTACGATAATGAACTGGTGGAAGCCGTTAAACGTTTTCAGGCATGGCAAGGATTGGGGGCAGATGGTGCTATTGGCCCGGCAACACGTGACTGGTTAAACGTAACGCCCGCCCAGCGTGCTGGTGTGTTGGCTCTCAACATCCAGCGATTGCGCTTGCTGCCAACAGAGCTTTCTACCGGGATCATGGTTAACATTCCGGCCTATTCGCTGGTCTACTATCAGAACGGCAATCAGGTGCTGGATTCGCGAGTCATTGTCGGTCGCCCCGATCGCAAAACGCCGATGATGAG  >  NC_000913/981994‑982284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑