Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,002,538 A→G V244V (GTA→GTG elfG → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,002,5380AG98.0% 167.3 / ‑6.8 50V244V (GTA→GTGelfGputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (29/20);  minor base T (1/0);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTC  >  NC_000913/1002404‑1002683
                                                                                                                                      |                                                                                                                                                 
aCGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTg                                                                                                                                   <  2:711864/151‑1 (MQ=255)
             cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                                                      >  2:744973/1‑151 (MQ=255)
             cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                                                      <  1:437365/151‑1 (MQ=255)
              acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGc                                                                                                                      >  2:656834/1‑150 (MQ=255)
                      tAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTg                                                                                                              <  2:566569/150‑1 (MQ=255)
                       aatgtATTACCTGTCTGGTACGATCAATTCAATTGGGTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGGTGATTTAGTTGATGTCTTAGCTGCCAATTTTCGTGTTGTTGGGCATAAAACTCTTGGGGCCAGAACGGCAGAACTTg                                                                                                              >  1:830693/3‑149 (MQ=255)
                       aGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc                                                                                                             <  2:440833/150‑1 (MQ=255)
                          gtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt                                                                                                          <  2:488039/150‑1 (MQ=255)
                          gtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt                                                                                                          <  1:701904/150‑1 (MQ=255)
                            aTTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc                                                                                                       >  1:184508/1‑151 (MQ=255)
                              tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc                                                                                                       >  2:615967/1‑149 (MQ=255)
                               aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt                                                                                                    >  2:57934/1‑151 (MQ=255)
                               aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt                                                                                                    >  2:207545/1‑151 (MQ=255)
                                ccTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc                                                                                                   >  1:795120/1‑151 (MQ=255)
                                 cTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc                                                                                                   >  2:24583/1‑150 (MQ=255)
                                  tGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc                                                                                                   >  1:161755/1‑149 (MQ=255)
                                   gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAgg                                                                                                >  1:726772/1‑151 (MQ=255)
                                   gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAgg                                                                                                >  2:727481/1‑151 (MQ=255)
                                        gTACGGACAATCCGCATGGCGCAGGGTCCGTCATTGCCGGAGAGACCCTTGAAGATGATTTAGGCGATGTCTTCGCTGACAGTTTCCGTGTTGTGGGGCATAAACCTGTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtgga                                                                                            >  1:359627/1‑148 (MQ=255)
                                            gATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACg                                                                                       >  2:355624/1‑151 (MQ=255)
                                             aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACgg                                                                                      >  1:479474/1‑151 (MQ=255)
                                                aaTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaa                                                                                   <  2:76252/151‑1 (MQ=255)
                                                aaTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGa                                                                                    >  2:397600/1‑150 (MQ=255)
                                                                   ccGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGt                                                                <  2:425745/151‑1 (MQ=255)
                                                                    cGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTc                                                               <  2:295850/151‑1 (MQ=255)
                                                                     gTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTc                                                               >  2:393225/1‑150 (MQ=255)
                                                                          tGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCt                                                          >  2:181335/1‑150 (MQ=255)
                                                                           gCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTg                                                         <  1:25923/150‑1 (MQ=255)
                                                                            ccGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAg                                                       <  2:225737/151‑1 (MQ=255)
                                                                                  agaCAATGGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAGTAGTTAATGTCAACCTGAGTCTGAc                                                 <  1:314096/151‑1 (MQ=255)
                                                                                     cAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg                                               >  2:185534/1‑150 (MQ=255)
                                                                                     cAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGc                                              <  1:243744/151‑1 (MQ=255)
                                                                                           aaGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACca                                         >  1:478112/1‑150 (MQ=255)
                                                                                           aaGTTGATTTAGGTGATGTCTTCGCTGCCAAGTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcac                                        <  1:9889/151‑1 (MQ=255)
                                                                                            aGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcaca                                       <  2:317530/151‑1 (MQ=255)
                                                                                                 aTTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAccc                                  >  2:821449/1‑151 (MQ=255)
                                                                                                   ttAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc                                 >  2:231882/1‑150 (MQ=255)
                                                                                                    tAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc                                 >  1:345161/1‑149 (MQ=255)
                                                                                                      ggTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCt                             >  2:9139/1‑151 (MQ=255)
                                                                                                         gATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGCCTGACGGCAACCACAGACCCCAGCTATc                          >  2:608392/1‑151 (MQ=255)
                                                                                                          aTGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATcc                         <  2:482443/151‑1 (MQ=255)
                                                                                                              cTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATcccc                       >  1:504409/1‑149 (MQ=255)
                                                                                                                 cGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCg                  <  1:49426/151‑1 (MQ=255)
                                                                                                                    tGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGAtt               >  2:277775/1‑151 (MQ=255)
                                                                                                                           ttCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgt        >  2:258443/1‑151 (MQ=255)
                                                                                                                            tCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgt        <  2:637855/150‑1 (MQ=255)
                                                                                                                             ccGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtc       <  1:370479/150‑1 (MQ=255)
                                                                                                                             ccGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtc       <  1:160600/150‑1 (MQ=255)
                                                                                                                              cGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtcac     >  1:196109/1‑151 (MQ=255)
                                                                                                                                 gttgtGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtcacgtc  >  2:456118/1‑151 (MQ=255)
                                                                                                                                      |                                                                                                                                                 
ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTC  >  NC_000913/1002404‑1002683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: