Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,002,538 | A→G | V244V (GTA→GTG) | elfG → | putative fimbrial‑like adhesin protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,002,538 | 0 | A | G | 98.0% | 167.3 / ‑6.8 | 50 | V244V (GTA→GTG) | elfG | putative fimbrial‑like adhesin protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (29/20); minor base T (1/0); total (30/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTC > NC_000913/1002404‑1002683 | aCGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTg < 2:711864/151‑1 (MQ=255) cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGc > 2:744973/1‑151 (MQ=255) cacCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGc < 1:437365/151‑1 (MQ=255) acCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGc > 2:656834/1‑150 (MQ=255) tAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTg < 2:566569/150‑1 (MQ=255) aatgtATTACCTGTCTGGTACGATCAATTCAATTGGGTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGGTGATTTAGTTGATGTCTTAGCTGCCAATTTTCGTGTTGTTGGGCATAAAACTCTTGGGGCCAGAACGGCAGAACTTg > 1:830693/3‑149 (MQ=255) aGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGc < 2:440833/150‑1 (MQ=255) gtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt < 2:488039/150‑1 (MQ=255) gtATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAAt < 1:701904/150‑1 (MQ=255) aTTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc > 1:184508/1‑151 (MQ=255) tACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTcc > 2:615967/1‑149 (MQ=255) aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt > 2:57934/1‑151 (MQ=255) aCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGt > 2:207545/1‑151 (MQ=255) ccTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc > 1:795120/1‑151 (MQ=255) cTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc > 2:24583/1‑150 (MQ=255) tGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTc > 1:161755/1‑149 (MQ=255) gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAgg > 1:726772/1‑151 (MQ=255) gTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAgg > 2:727481/1‑151 (MQ=255) gTACGGACAATCCGCATGGCGCAGGGTCCGTCATTGCCGGAGAGACCCTTGAAGATGATTTAGGCGATGTCTTCGCTGACAGTTTCCGTGTTGTGGGGCATAAACCTGTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGgtgga > 1:359627/1‑148 (MQ=255) gATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACg > 2:355624/1‑151 (MQ=255) aTCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACgg > 1:479474/1‑151 (MQ=255) aaTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGaa < 2:76252/151‑1 (MQ=255) aaTTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGa > 2:397600/1‑150 (MQ=255) ccGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGt < 2:425745/151‑1 (MQ=255) cGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTc < 2:295850/151‑1 (MQ=255) gTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTc > 2:393225/1‑150 (MQ=255) tGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCt > 2:181335/1‑150 (MQ=255) gCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTg < 1:25923/150‑1 (MQ=255) ccGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAg < 2:225737/151‑1 (MQ=255) agaCAATGGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGAGTAGTTAATGTCAACCTGAGTCTGAc < 1:314096/151‑1 (MQ=255) cAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACgg > 2:185534/1‑150 (MQ=255) cAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGc < 1:243744/151‑1 (MQ=255) aaGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACca > 1:478112/1‑150 (MQ=255) aaGTTGATTTAGGTGATGTCTTCGCTGCCAAGTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcac < 1:9889/151‑1 (MQ=255) aGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACcaca < 2:317530/151‑1 (MQ=255) aTTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAccc > 2:821449/1‑151 (MQ=255) ttAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc > 2:231882/1‑150 (MQ=255) tAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGAcccc > 1:345161/1‑149 (MQ=255) ggTGATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCt > 2:9139/1‑151 (MQ=255) gATGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGCCTGACGGCAACCACAGACCCCAGCTATc > 2:608392/1‑151 (MQ=255) aTGTCTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATcc < 2:482443/151‑1 (MQ=255) cTTCGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATcccc > 1:504409/1‑149 (MQ=255) cGCTGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCg < 1:49426/151‑1 (MQ=255) tGCCAATTTCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGAtt > 2:277775/1‑151 (MQ=255) ttCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgt > 2:258443/1‑151 (MQ=255) tCCGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgt < 2:637855/150‑1 (MQ=255) ccGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtc < 1:370479/150‑1 (MQ=255) ccGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtc < 1:160600/150‑1 (MQ=255) cGTGTTGTGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtcac > 1:196109/1‑151 (MQ=255) gttgtGGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGacgtcacgtc > 2:456118/1‑151 (MQ=255) | ACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACGGCAGAACTTGCAATTCCAGTCAGGTGTAACACGGGAAACGCGGGATTAGTTAATGTCAACCTGAGTCTGACGGCAACCACAGACCCCAGCTATCCCCAGGCGATTAAGACGTCACGTC > NC_000913/1002404‑1002683 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |