Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,022,227	A→G	Y553H (TAT→CAT)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,022,227	0	A	G	100.0%	132.7 / NA	37	Y553H (TAT→CAT)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

GGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTC  >  NC_000913/1022087‑1022374                                                                                                                                             |                                                                                                                                                    ggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTg                                                                                                                                           <  1:426951/151‑1 (MQ=255) ggTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTg                                                                                                                                           <  1:73227/151‑1 (MQ=255)         tCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCtg                                                                                                                                    <  1:77576/150‑1 (MQ=255)           ggCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTgg                                                                                                                                 >  2:692243/1‑151 (MQ=255)                gCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGt                                                                                                                            >  1:620034/1‑151 (MQ=255)                   ggACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTc                                                                                                                          <  1:376173/150‑1 (MQ=255)                    gACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCg                                                                                                                         >  2:193482/1‑150 (MQ=255)                       atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCa                                                                                                                     <  1:673008/151‑1 (MQ=255)                       atatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAAATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGc                                                                                                                      >  2:311444/1‑150 (MQ=255)                        tatTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGCTACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCAt                                                                                                                    >  1:746876/1‑151 (MQ=255)                         atTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATg                                                                                                                   >  1:161695/1‑151 (MQ=255)                               tACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCgg                                                                                                              >  1:478486/1‑150 (MQ=255)                                    ccGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGAtt                                                                                                        >  2:412098/1‑151 (MQ=255)                                      gACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGc                                                                                                      >  1:621339/1‑151 (MQ=255)                                            aGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACt                                                                                                <  2:513059/151‑1 (MQ=255)                                                cAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCa                                                                                            <  1:115555/151‑1 (MQ=255)                                                  gCATCACGGTTGTGTGCATCGCGGCGGGCAATAGGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                          <  2:160648/151‑1 (MQ=255)                                                  gCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                          <  2:319150/151‑1 (MQ=255)                                                              tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGGATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCACTCAGGCCAGATGt                                                                              >  1:384838/1‑151 (MQ=255)                                                                               aaTACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCAc                                                             <  2:815778/151‑1 (MQ=255)                                                                                atacgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACg                                                            <  2:784920/151‑1 (MQ=255)                                                                                  acgatacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCa                                                          <  2:416453/151‑1 (MQ=255)                                                                                        cgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTTGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCACACGCAATgt                                                      <  1:483002/149‑2 (MQ=255)                                                                                           cataCGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGcaca                                                  >  2:595935/4‑150 (MQ=255)                                                                                           cAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACaa                                                 <  2:400238/151‑1 (MQ=255)                                                                                            aGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAAc                                                >  2:282835/1‑151 (MQ=255)                                                                                               cGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCa                                              >  2:679201/1‑150 (MQ=255)                                                                                                              tGATGGTATTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGAtt                               >  2:461416/1‑150 (MQ=255)                                                                                                                     aTTGCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAg                        <  1:555614/150‑1 (MQ=255)                                                                                                                        gCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCTGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTa                     >  2:690335/1‑150 (MQ=255)                                                                                                                        gCTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTa                     >  2:575851/1‑150 (MQ=255)                                                                                                                         cTCCAGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAAc                   >  2:158012/1‑151 (MQ=255)                                                                                                                             aGTATGGCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCt                >  2:337676/1‑150 (MQ=255)                                                                                                                                   gCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaa         <  2:298201/151‑1 (MQ=255)                                                                                                                                   gCATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCa          >  1:715398/1‑150 (MQ=255)                                                                                                                                    cATCGAGATGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaa         >  2:172164/1‑150 (MQ=255)                                                                                                                                          gaTGCCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTc  <  1:692200/151‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    GGTAACGTTCGGCTCGCTGGACATATTTGATACCACCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTC  >  NC_000913/1022087‑1022374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑