Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,028,234 A→G T97A (ACT→GCT)  yccU → putative CoA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,028,2340AG98.0% 176.0 / NA 51T97A (ACT→GCT) yccUputative CoA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (28/22);  minor base C (1/0);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGG  >  NC_000913/1028088‑1028370
                                                                                                                                                  |                                                                                                                                        
cAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGGTGGCAGACGTTCCGGACAAAGTCGATATGGACGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGACCTTGGCGAGAAACctc                                                                                                                                       >  1:160153/1‑150 (MQ=255)
     gTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTgg                                                                                                                                  >  2:601683/1‑150 (MQ=255)
     gTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGa                                                                                                                                 <  2:4903/151‑1 (MQ=255)
           ggCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAAc                                                                                                                           <  2:372041/151‑1 (MQ=255)
                      gctgGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGAt                                                                                                                 <  1:16845/150‑1 (MQ=255)
                        tgGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTa                                                                                                               >  1:263525/1‑150 (MQ=255)
                         gggCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAAt                                                                                                             >  2:368716/1‑151 (MQ=255)
                           gCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGCGATTAATg                                                                                                            >  1:40906/1‑150 (MQ=255)
                               gAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCaa                                                                                                       <  2:362608/151‑1 (MQ=255)
                                     gTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGc                                                                                                  >  2:545009/1‑150 (MQ=255)
                                      tATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGc                                                                                                  >  2:453792/1‑149 (MQ=255)
                                      tATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGc                                                                                                  >  1:513884/1‑149 (MQ=255)
                                         ggCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTAc                                                                                             <  2:519784/151‑1 (MQ=255)
                                            aCGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTgg                                                                                          <  2:286868/151‑1 (MQ=255)
                                              gCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGc                                                                                         >  2:130172/1‑150 (MQ=255)
                                               cTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCAc                                                                                       >  2:337079/1‑151 (MQ=255)
                                                tGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACg                                                                                      >  2:231385/1‑151 (MQ=255)
                                                 ggCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACgg                                                                                     >  1:250657/1‑151 (MQ=255)
                                                    aGACGTTCCGGAAAAAGCCGATATGGTCGATGTTTTCCGCAATTCAGAACCGGCGTGGGGCGTGGCACAGTAAGCTATTGCCATTGGCGCGAAAGCCCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACggg                                                                                    >  1:303899/1‑149 (MQ=255)
                                                     gACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATg                                                                                 >  2:14934/1‑151 (MQ=255)
                                                      aCGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGc                                                                                <  2:568576/151‑1 (MQ=255)
                                                         ttCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCgg                                                                             >  2:70461/1‑151 (MQ=255)
                                                           ccGGAAAAAGTCAATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCGCAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTTCTGGCACGGGATGCCgt                                                                             <  1:770582/149‑2 (MQ=255)
                                                               aaaaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTCCTGGCACGGGATGCCGGGTTaaa                                                                       <  1:530760/151‑1 (MQ=255)
                                                                 aaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAAtg                                                                     >  2:713269/1‑151 (MQ=255)
                                                                 aaaGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAAtg                                                                     <  1:544668/151‑1 (MQ=255)
                                                                       gATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATgtggtg                                                                >  2:83013/1‑150 (MQ=255)
                                                                       gATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGa                                                               <  1:452045/151‑1 (MQ=255)
                                                                          atGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATgg                                                            <  2:752024/151‑1 (MQ=255)
                                                                             gTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATc                                                         <  1:816722/151‑1 (MQ=255)
                                                                               cGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGc                                                       >  1:482813/1‑151 (MQ=255)
                                                                                gATGTTTTCCGTAATTCAGAAGCGGCGTGGGGTGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCt                                                      >  1:30495/1‑151 (MQ=255)
                                                                                 aTGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTg                                                     >  2:688697/1‑151 (MQ=255)
                                                                                    ttttCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGa                                                                                   <  1:113915/118‑1 (MQ=255)
                                                                                    ttttCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGa                                                                                   >  2:113938/1‑118 (MQ=255)
                                                                                       tCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCgg                                                <  1:30614/150‑1 (MQ=255)
                                                                                        ccGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGc                                               <  2:616201/150‑1 (MQ=255)
                                                                                           tAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTAtt                                           >  2:46099/1‑151 (MQ=255)
                                                                                             aTTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTga                                         <  2:490729/151‑1 (MQ=255)
                                                                                                 aGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGGGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTg                                          >  1:730551/1‑146 (MQ=255)
                                                                                                            ggggcgtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctgg                           <  1:46077/150‑1 (MQ=255)
                                                                                                                 gtggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCgcctgggcctgg                     >  1:15015/1‑151 (MQ=255)
                                                                                                                  tggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGc                    >  1:361118/1‑151 (MQ=255)
                                                                                                                   ggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGcc                   <  2:327688/151‑1 (MQ=255)
                                                                                                                   ggCACAGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGc                    <  1:544972/150‑1 (MQ=255)
                                                                                                                       caGGAAGCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAAt               >  2:49878/1‑151 (MQ=255)
                                                                                                                             gCTATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATaaaaa          >  2:26429/1‑150 (MQ=255)
                                                                                                                               tATTGCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATc       >  2:129713/1‑151 (MQ=255)
                                                                                                                                   gCCATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCg   <  1:31822/151‑1 (MQ=255)
                                                                                                                                     cATTGGCGCGAAAGCTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCgg  >  1:555261/1‑150 (MQ=255)
                                                                                                                                      aTTGGCGCGAAAGCTGTCTGGATGCAACTGGGGTTGATTAATGAGCAAGCGGCAGTACGGGCGCGGGATGCTGGGTTAAATGTGCTTATGGATCGCTGGCCGTCTATTGAGATCGCTCGCCTGGGGCTGGCCAAATAAAAAATCCCCgg  <  1:568796/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
CAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCTCTGGATGCAACTGGGGGTGATTAATGAGCAAGCGGCAGTACTGGCACGGGATGCCGGGTTAAATGTGGTGATGGATCGCTGCCCGGCTATTGAGATCCCTCGCCTGGGCCTGGCCAAATAAAAAATCCCCGG  >  NC_000913/1028088‑1028370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑