Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,151,418 A→G intergenic (+14/‑197) fabG → / → acpP 3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,4180AG98.3% 205.4 / NA 58intergenic (+14/‑197)fabG/acpP3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (22/35);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTTAT  >  NC_000913/1151272‑1151564
                                                                                                                                                  |                                                                                                                                                  
ggCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACgaaa                                                                                                                                                 <  1:338701/150‑1 (MQ=255)
   gcAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCt                                                                                                                                             >  2:19867/1‑151 (MQ=255)
   gcAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCt                                                                                                                                             <  2:79078/151‑1 (MQ=255)
     aGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCa                                                                                                                                           <  2:823065/151‑1 (MQ=255)
          ccTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTg                                                                                                                                      >  1:571084/1‑151 (MQ=255)
          ccTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTg                                                                                                                                      <  2:519763/151‑1 (MQ=255)
                ggTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTgcgcgc                                                                                                                                 >  1:686943/1‑150 (MQ=255)
                ggTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCa                                                                                                                                <  2:369285/151‑1 (MQ=255)
                    gCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCt                                                                                                                            <  2:468977/151‑1 (MQ=255)
                        cggcggCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGc                                                                                                                          <  2:615956/149‑1 (MQ=255)
                        cggcggCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCg                                                                                                                         <  2:631318/150‑1 (MQ=255)
                         ggcggcGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTg                                                                                                                       <  1:556006/151‑1 (MQ=255)
                             gcgcACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTa                                                                                                                   <  2:374890/151‑1 (MQ=255)
                                cacaGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                                                                                                 >  1:835003/1‑150 (MQ=255)
                                cacaGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATg                                                                                                                 >  1:701432/1‑150 (MQ=255)
                                cacaGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGa                                                                                                                <  2:529780/151‑1 (MQ=255)
                                   aGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGt                                                                                                              >  1:177495/1‑150 (MQ=255)
                                     gAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGtaat                                                                                                           <  2:734675/151‑1 (MQ=255)
                                        aTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTaataat                                                                                                        <  1:770225/151‑1 (MQ=255)
                                          cGCCAACGCGGTTGCATTCCTTGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTa                                                                                                      <  2:500495/151‑1 (MQ=255)
                                          cGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAAtt                                                                                                       <  1:14983/150‑1 (MQ=255)
                                                    gTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATg                                                                                            <  2:722747/151‑1 (MQ=255)
                                                       gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAtt                                                                                         >  2:638686/1‑151 (MQ=255)
                                                       gCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAt                                                                                          <  1:10589/150‑1 (MQ=255)
                                                         aTTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGAttt                                                                                        >  1:574053/1‑150 (MQ=255)
                                                           tCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGc                                                                                      <  2:654250/150‑1 (MQ=255)
                                                               ggCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTAt                                                                                 >  2:394289/1‑151 (MQ=255)
                                                                 cATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTg                                                                               <  1:61374/151‑1 (MQ=255)
                                                                  aTCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTgg                                                                              >  2:40915/1‑151 (MQ=255)
                                                                         gaAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTaa                                                                       <  2:411344/151‑1 (MQ=255)
                                                                           agcagcTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAgg                                                                     <  1:514606/151‑1 (MQ=255)
                                                                                 ttACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCa                                                                >  1:419660/1‑150 (MQ=255)
                                                                                     aTCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAAt                                                            >  1:621310/1‑150 (MQ=255)
                                                                                     aTCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAAt                                                            >  2:21423/1‑150 (MQ=255)
                                                                                     aTCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATa                                                           <  1:719231/151‑1 (MQ=255)
                                                                                       cACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATaa                                                          <  2:708991/150‑1 (MQ=255)
                                                                                            gTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTa                                                      >  2:829066/1‑149 (MQ=255)
                                                                                              gAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAAt                                                  <  2:805355/151‑1 (MQ=255)
                                                                                                    ttGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTgg                                             >  1:625562/1‑150 (MQ=255)
                                                                                                        aTGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTa                                           >  2:417677/1‑148 (MQ=255)
                                                                                                        aTGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGa                                        <  2:251684/151‑1 (MQ=255)
                                                                                                          gtgAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGAc                                       <  2:702131/150‑1 (MQ=255)
                                                                                                            gAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCt                                     <  2:726814/150‑1 (MQ=255)
                                                                                                            gAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTg                                    <  1:834008/151‑1 (MQ=255)
                                                                                                             aaCGGCGGGGTGTAAATGGTCTTACCGCGATTTTTACGAAATGCTCATGTTGCGCGCAGTCTGCGGGGGTTTGAGTAATAATTAGTGCAAAATGATTTGCGGTATTTGGGGGGAAGGCCTCAAAATAACGTAAAATAGTGGTAAGAcc                                      >  1:848473/1‑148 (MQ=255)
                                                                                                                ggcggGATGTACATGGTCTGACGGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCg                                 <  1:605442/150‑1 (MQ=255)
                                                                                                                 gcggGATGTACATGGTCTGACCGCGATTTGCACGAAACGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCggg                               <  1:690641/151‑1 (MQ=255)
                                                                                                                     gATGTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGAttt                           <  1:119545/151‑1 (MQ=255)
                                                                                                                        gTACATGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGt                        >  2:851252/1‑151 (MQ=255)
                                                                                                                            aTGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGc                     >  2:813162/1‑150 (MQ=255)
                                                                                                                            aTGGTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCa                    <  1:542349/151‑1 (MQ=255)
                                                                                                                              ggTCTGGCCGCGATTTGCACAAAATGCTTATGTTGCGCGCTGTTTGCGTGGTTATGAGTAATAATTAGTGCATAATGATTTGCGTTCTTGGGCGGTACGGCCTCCAAACACCCTAACATCGTGATAAGACCCGCCGGGATTTAGTTTCat                   <  1:296569/150‑2 (MQ=255)
                                                                                                                              ggTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCaaa                  <  2:687859/151‑1 (MQ=255)
                                                                                                                              ggTCTGACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCaaa                  <  1:723680/151‑1 (MQ=255)
                                                                                                                                   gACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAAttttt             >  1:806898/1‑151 (MQ=255)
                                                                                                                                   gACCGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAAttt               <  1:788300/149‑1 (MQ=255)
                                                                                                                                     ccGCGATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTc            >  1:307665/1‑150 (MQ=255)
                                                                                                                                       gcgATTTGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAAc         <  2:769184/151‑1 (MQ=255)
                                                                                                                                             tGCACGAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACAtttt    >  2:787667/1‑150 (MQ=255)
                                                                                                                                               cacgAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTtat  >  2:230652/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAACGGCGGGATGTACATGGTCTGACCGCGATTTGCACAAAATGCTCATGTTGCGCGCAGTCTGCGTGGTTATGAGTAATAATTAGTGCAAAATGATTTGCGTTATTGGGGGGTAAGGCCTCAAAATAACGTAAAATCGTGGTAAGACCTGCCGGGATTTAGTTGCAAATTTTTCAACATTTTAT  >  NC_000913/1151272‑1151564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑