Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,174,596	A→G	S190S (AGT→AGC)	ycfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,596	0	A	G	98.5%	195.2 / ‑4.3	68	S190S (AGT→AGC)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (38/28);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

AGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAG  >  NC_000913/1174448‑1174737                                                                                                                                                     |                                                                                                                                               agaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTATTGCGTCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCt                                                                                                                                              <  1:385580/151‑1 (MQ=255)    tAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGtt                                                                                                                                           >  2:249544/1‑151 (MQ=255)    tAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGtt                                                                                                                                           >  2:542875/1‑151 (MQ=255)     aGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGtt                                                                                                                                           <  2:693427/150‑1 (MQ=255)        aaaCAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAGGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCAtt                                                                                                                                       >  2:251960/1‑151 (MQ=255)           caacCAGGCACCGACCGCCAGAACGGAAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGACATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCAtt                                                                                                                                       >  2:363545/1‑148 (MQ=255)              ccAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCccac                                                                                                                                >  1:118075/1‑151 (MQ=255)                   cACCGCCCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCAGCTCTTTAACACAGGACATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACgg                                                                                                                           >  1:222136/1‑151 (MQ=255)                      cgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGt                                                                                                                        >  1:412388/1‑151 (MQ=255)                           gCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGaa                                                                                                                   <  2:573154/151‑1 (MQ=255)                                aaCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGaaa                                                                                                              <  1:89672/151‑1 (MQ=255)                                  cGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAAtt                                                                                                            <  2:452654/151‑1 (MQ=255)                                     tAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAAc                                                                                                         >  2:49092/1‑151 (MQ=255)                                     tAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAAc                                                                                                         >  1:711944/1‑151 (MQ=255)                                          aaaGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCca                                                                                                    <  1:312657/151‑1 (MQ=255)                                           aagaagCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCcac                                                                                                   >  2:208837/1‑151 (MQ=255)                                            agaagCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCcac                                                                                                   >  1:783624/1‑150 (MQ=255)                                              aagCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCacac                                                                                                 <  1:234964/150‑1 (MQ=255)                                              aagCCATCAGCAGAGGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCAAACCCATGCGCCAGGGCTGTAATAAGGAAAATTGCGGACCCCGCTGTTCATC‑CCCCCCCACGGCGTGGAAACAAAATTAACCGCCACACt                                                                                                <  1:630409/151‑1 (MQ=255)                                                 ccATCAGCAGATGGCGGCGCACCGGCCCCTTTTTAACACAGGTAATTATTGTTGCGCAAACCCATGCCCCAAGGCTGAAAAAAGGAAAATTGCGGATCCCGCTGTTCATT‑CCCCACCCCGGCGTGGGAACGAAATTAACCGCCACACTc                                                                                               <  1:35794/149‑1 (MQ=255)                                                   aTCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag                                                                                             >  2:327678/1‑149 (MQ=255)                                                    tCAGCAGAGGGCGGCGCAACGGCCCCTCTTTAACACGGTTCATTATTGTTGCGCAAACCCATGCCCCAAGGCTGTAATAAGGCAAATTGCGAATCACGCTGTTCATC‑CCCCCCCACGGCGTGGAAACAAAATTAACCGCCACACTcagca                                                                                           <  2:593055/150‑1 (MQ=255)                                                    acagcagAGGGCGGCGAAACGGCCCCTCTTTAACACAGGTCATTTTTGTTGCGCCAAACCAGGCGCCAGGGCTGAAATAAGCCAAATGGCAAACCCCGCTGTTCTTC‑CCCCCCCACGGCGGGGGAAAGAAATTAACCGCCACACTcagcag                                                                                          <  1:723939/150‑1 (MQ=255)                                                        cagATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTac                                                                                       >  2:366165/1‑150 (MQ=255)                                                         agagGGCGGCGCAACGGCCCCTTTTAAACACAGGTCATTTTTGTTGCCCCAACCCATGCGCAAAGGCTGAAATAAGGAAAATTGGGGATCACGCTTTTCATC‑CCCCCCCCCGGCGTGGAAACAAAATTAACCGCCACACTCAGCAGTaca                                                                                      <  2:447859/150‑1 (MQ=255)                                                         agATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTaca                                                                                      >  1:831489/1‑150 (MQ=255)                                                             ggcggcCCAACGGCCCCTTTTAAACACAGGTCTTTATTTTTGCGCAAACCCAGGCGCCAAGGCTGAAATAAGGCAAATTGCGGATCCCGCTGTTCATC‑CCCCCCCACGGCGGGGGAACGAAATTAACCGCCACACTCAGCAGTacaaacaa                                                                                 <  2:803006/151‑1 (MQ=255)                                                                  cgcAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCg                                                                             >  2:416323/1‑150 (MQ=255)                                                                           aCCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGTTGTAATAAGGCAAATTGCGGATCACGCTGTTCCTT‑CCCCACCACGGCGTGGGAACGAAAATAACCGCGACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                    >  1:71677/1‑150 (MQ=255)                                                                              tctTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgcg                                                                >  2:564859/1‑151 (MQ=255)                                                                                tttAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGcc                                                              >  2:769724/1‑151 (MQ=255)                                                                                ttaaaaacaGTTCATTATTGTTGCGCCAAACCATGCGCCAGGGCTATAATAAGGAAAATGGCGGATCCCGCTGTTCATC‑CCCCACCCCGGCGGGGAAACGAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgcgc                                                               <  1:117128/144‑1 (MQ=255)                                                                                  tAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGcc                                                              >  1:576332/1‑149 (MQ=255)                                                                                  tAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGcc                                                              >  1:290898/1‑149 (MQ=255)                                                                                   aaCACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGcc                                                              >  2:651819/1‑148 (MQ=255)                                                                                   aaCACAGGTAATTATTGTTGCGCAAAACAATGCGCAAAGGTTGTAAAAAGGAAAATTGCGGATCACGTTTTTCATC‑CCCCACCCCGGCGGGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCa                                                                   <  1:293289/143‑1 (MQ=255)                                                                                       caGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGc                                                       >  1:143232/1‑151 (MQ=255)                                                                                         ggactttattGTTCCGCAAACCCATGCCCAAAGGTTGAAATAAGGAAATTTGCGTATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTa                                                     <  2:454354/146‑1 (MQ=255)                                                                                         ggTCTTTATTGTTCCGCAAAACCCGCCGCCAGGGCTGTAATAGGGCAAGTTGCGTATCGCGCTG‑TCATTCCCCCACCCGGGCGTGGGAACGAAATTATCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTa                                                     <  1:100997/151‑1 (MQ=255)                                                                                          gTCATTGTTGTCGGCCAAAACCATGCCCCAAGACAGTAAGAAGGCAAGTTGCGGCACCGGCTCTGCATC‑CCCCCCAACGGCGTGGAACAGAAAATAGCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                                    <  1:53414/151‑1 (MQ=255)                                                                                          gTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGCCGGCTa                                                     >  1:605658/1‑150 (MQ=255)                                                                                            cATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaa                                                   >  1:415852/1‑150 (MQ=255)                                                                                             attattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaa                                                  >  2:441960/1‑150 (MQ=255)                                                                                                 ttgttgCGCCAAACCATGCGCCAAGGCTGAAAAAAGGAAAATTGCGGATCACGCTGTTCATC‑CCCCCCCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAttt                                              <  1:49070/150‑1 (MQ=255)                                                                                                 ttgttgCCCAAACCCATGCGCCAAGGCTGTAATAAGGAAAATGGGGGATCACGCTGTTCATC‑CCCCCCCACGGCGTGGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAttt                                              <  2:155940/150‑1 (MQ=255)                                                                                                    ttgCGCAAAACCATGCGCAAGGGCTGTAATAAGGAAAATTGCGGATCACGCTGTTCATC‑CCCCCCCACGGGGTGGAAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTAc                                           <  1:162796/150‑1 (MQ=255)                                                                                                      gcgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACAt                                         >  1:551682/1‑150 (MQ=255)                                                                                                       cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAc                                       >  1:295303/1‑151 (MQ=255)                                                                                                        gcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                                      >  2:335900/1‑151 (MQ=255)                                                                                                           aacccagcccccAGGGCTGTAAAAAGGCAATTTGCGAATCACGTTGTTCTTC‑CCCCCCCCCGGGGGGGAAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCAc                                    <  2:349857/140‑1 (MQ=255)                                                                                                           aaaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACg                                   >  2:699742/1‑151 (MQ=255)                                                                                                           aaaCCATGCGCCAAGGCTGTAAAAAGGAAAATGGCGGATCCCGTTGTTCATC‑CCCCCCCGCGGGGGGGAAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCAc                                    <  1:152104/150‑1 (MQ=255)                                                                                                              ccATGCGCCAGGGCTGTAATAAGGAAAATTGCGGATCACGCTTTTCATC‑CCCCCCCACGGCGTGGGAAAGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaaa                                <  1:233110/151‑1 (MQ=255)                                                                                                              ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaa                                 >  2:805721/1‑150 (MQ=255)                                                                                                               cATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaaa                                >  1:130402/1‑150 (MQ=255)                                                                                                                    gcCAAGGCTGTAATAAGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACTGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGa                            >  1:491662/1‑149 (MQ=255)                                                                                                                       aaGGCTGTAAAAAGGCAAATTGCGGATCCCGCTGTTCATC‑CCCCACCCCGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAtt                       <  2:248116/151‑1 (MQ=255)                                                                                                                         ggCTTTAAAAAAGAAAATGGGGGACCACGTTGTTCATC‑CCCCCCCGCGGGGTGGAAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGa                            <  1:4769/144‑1 (MQ=255)                                                                                                                              taataaGGCAAATTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCa                 >  2:445628/1‑150 (MQ=255)                                                                                                                                  aaGGAAAATGGGGGATCCCGCTGTTCATC‑CCCCCCCACGGGGGGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAg            <  1:551691/151‑1 (MQ=255)                                                                                                                                   aGGCAAATTGCGGATCACACTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATAc              >  2:174600/1‑148 (MQ=255)                                                                                                                                    ggaaaaTTGCGGACCACGCTTTTCATC‑CCCCACCACGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGAt          <  2:676093/148‑1 (MQ=255)                                                                                                                                       aaaTTGCGGATCACGCTGATCATC‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCAAGACGGCTAAAAATTTTACATAACACGAAATAGACAATTAACGCATACAGATa         >  1:675899/1‑149 (MQ=255)                                                                                                                                        aaTTGGGGATCACTCTTTTCATC‑CCCCCCCACGGGGGGGAAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACca      <  1:335877/151‑1 (MQ=255)                                                                                                                                        aaTTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAcc       >  2:177829/1‑150 (MQ=255)                                                                                                                                         aTTGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACcac     >  1:850138/1‑151 (MQ=255)                                                                                                                                           tGCGGATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACcaca    >  2:362511/1‑150 (MQ=255)                                                                                                                                               gATCACGCTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGCACAAAAAAGGCGAATAGTGGCAGCGCAAGACGGCTAAAAATTTTACATACCACGAAATATAAAATTAAAGCATACAGATACAACAAg  >  1:736849/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                               AGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATT‑CCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAG  >  NC_000913/1174448‑1174737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑