Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,192,714	A→G	C354R (TGC→CGC)	mnmA ←	tRNA(Gln,Lys,Glu) U34 2‑thiouridylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,192,714	0	A	G	100.0%	188.5 / NA	54	C354R (TGC→CGC)	mnmA	tRNA(Gln,Lys,Glu) U34 2‑thiouridylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/23);  total (31/23)

CACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCAACG  >  NC_000913/1192566‑1192861                                                                                                                                                     |                                                                                                                                                    cACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGcgca                                                                                                                                                   <  1:192110/151‑1 (MQ=255)     aGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTc                                                                                                                                               >  2:351434/1‑151 (MQ=255)            ccGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGtt                                                                                                                                         >  2:122487/1‑150 (MQ=255)              gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTtata                                                                                                                                      <  1:363071/151‑1 (MQ=255)              gACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTtat                                                                                                                                       >  1:734366/1‑150 (MQ=255)                 tGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATaga                                                                                                                                    >  1:157131/1‑150 (MQ=255)                  gACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATCCCGCCACCGAGGCGCACTTCACCGTTATagaag                                                                                                                                  <  1:707585/151‑1 (MQ=255)                   aCAAATGCCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAAGCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATagaaga                                                                                                                                 <  1:271822/151‑1 (MQ=255)                       aTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGc                                                                                                                             >  1:315782/1‑151 (MQ=255)                       aTACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGc                                                                                                                             <  2:648715/151‑1 (MQ=255)                          ccGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGc                                                                                                                             >  1:284180/1‑148 (MQ=255)                            ggCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACt                                                                                                                        >  2:90991/1‑151 (MQ=255)                               cAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGc                                                                                                                     >  1:317548/1‑151 (MQ=255)                                 gggCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGccc                                                                                                                   >  1:176193/1‑151 (MQ=255)                                       gggTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTc                                                                                                             <  1:137634/151‑1 (MQ=255)                                              gTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcggcgg                                                                                                      >  2:402131/1‑151 (MQ=255)                                                 aTAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCAcggc                                                                                                        >  2:666882/1‑146 (MQ=255)                                                            ttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc                                                                                         >  2:558844/1‑150 (MQ=255)                                                            ttGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc                                                                                         <  1:350933/150‑1 (MQ=255)                                                                        cTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTc                                                                             >  2:88137/1‑150 (MQ=255)                                                                            ttCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGc                                                                        >  2:593122/1‑151 (MQ=255)                                                                              ccTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGa                                                                      <  2:181248/151‑1 (MQ=255)                                                                               cTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGat                                                                     >  2:470504/1‑151 (MQ=255)                                                                               cTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGat                                                                     >  2:223074/1‑151 (MQ=255)                                                                                ttGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGat                                                                     >  2:264401/1‑150 (MQ=255)                                                                                    tttAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGatcatc                                                                 >  1:52057/1‑150 (MQ=255)                                                                                    tttAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCg                                                                >  2:529967/1‑151 (MQ=255)                                                                                       aaGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTc                                                              <  2:67929/150‑1 (MQ=255)                                                                                           aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc                                                                                         <  2:254970/119‑1 (MQ=255)                                                                                           aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATc                                                                                         >  1:254945/1‑119 (MQ=255)                                                                                           aaaGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAgcg                                                         <  1:490430/151‑1 (MQ=255)                                                                                              gATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCCTCGAAAATCACTTCAATGCGATCATCGTCCAgcgc                                                        >  1:467968/1‑149 (MQ=255)                                                                                              gATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCt                                                      >  1:500502/1‑151 (MQ=255)                                                                                                 aataatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTg                                                    <  2:561002/150‑1 (MQ=255)                                                                                                    aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACgg                                                <  2:521785/151‑1 (MQ=255)                                                                                                    aatCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACg                                                 <  2:705795/150‑1 (MQ=255)                                                                                                     atCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACgg                                                >  2:167020/1‑150 (MQ=255)                                                                                                     atCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACgg                                                >  2:850556/1‑150 (MQ=255)                                                                                                           ccGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAgg                                         <  2:525240/151‑1 (MQ=255)                                                                                                            cggcagcggcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAggg                                        >  2:846502/1‑151 (MQ=255)                                                                                                             ggcagcggcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAggg                                        >  2:39022/1‑150 (MQ=255)                                                                                                                   ggcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCgg                                 <  2:317572/151‑1 (MQ=255)                                                                                                                   ggcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCgg                                 <  1:428397/151‑1 (MQ=255)                                                                                                                    gcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGt                                <  1:360726/151‑1 (MQ=255)                                                                                                                    gcagACGCTGCTCAATAATACCGCCACCGAGGCGCACTTCAAAGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGATCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGGGCAAGGGATATCgg                                 >  2:153698/1‑150 (MQ=255)                                                                                                                         cGCTGCTCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGc                           <  2:303550/151‑1 (MQ=255)                                                                                                                          gctgctCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCg                          >  1:519248/1‑151 (MQ=255)                                                                                                                           ctgctCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCg                          >  1:376098/1‑150 (MQ=255)                                                                                                                             gctCAATAATACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGAt                        <  1:54004/150‑1 (MQ=255)                                                                                                                                 aataatACCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCg                    <  1:424930/150‑1 (MQ=255)                                                                                                                                    aataCCGCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGtt                >  1:462909/1‑151 (MQ=255)                                                                                                                                          gCCACCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTAcc          <  1:526307/151‑1 (MQ=255)                                                                                                                                             aCCGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTg       >  2:192801/1‑151 (MQ=255)                                                                                                                                              ccGAGGCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCAGCGTCCAGCGCCGTGACGGTGCAAGGGATATCGGGCTGGCGATAGGGGGTTTTTACCg         <  1:33712/148‑1 (MQ=255)                                                                                                                                                  ggCGCACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCAACg  <  1:90968/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCCTTGTTTTAAGTAAAGATAATAATCAGACCGGCAGCGGCAGACGCTGCTCAATAATACCGCCACCGAGGCACACTTCACCGTTATAGAAGACGGCAGACTGGCCCGGCGTCACGGCGGCAACCGGTTCATCGAAAATCACTTCAATGCGATCATCGTCCAGCGCCTTGACGGTGCAAGGGATATCGGTCTGGCGATAGCGGGTTTTTACCGTGCAACG  >  NC_000913/1192566‑1192861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑