Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,217,003	A→G	intergenic (+7/‑325)	ymgC → / → ycgG	Blue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,217,003	0	A	G	100.0%	241.7 / NA	64	intergenic (+7/‑325)	ymgC/ycgG	Blue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/31);  total (33/31)

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCG  >  NC_000913/1216856‑1217148                                                                                                                                                    |                                                                                                                                                  cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGt                                                                                                                                                 <  1:72700/150‑1 (MQ=255) cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTa                                                                                                                                                <  2:99834/151‑1 (MQ=255)  aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTaa                                                                                                                                               <  1:632354/151‑1 (MQ=255)        tACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGtttt                                                                                                                                         >  1:340244/1‑151 (MQ=255)          cGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGAGAATCCGTGCTATCTTAGAACTTGGGAAAGGtttt                                                                                                                                         >  1:725342/1‑149 (MQ=255)           gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGtttt                                                                                                                                         >  1:295933/1‑148 (MQ=255)            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGa                                                                                                                                      >  1:519089/1‑150 (MQ=255)             ttGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAAt                                                                                                                                    >  2:362422/1‑151 (MQ=255)              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGaa                                                                                                                                     <  2:46252/149‑1 (MQ=255)                     aaaaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATc                                                                                                                             <  1:367826/150‑1 (MQ=255)                            gAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTAt                                                                                                                     >  2:507478/1‑151 (MQ=255)                                ccAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                                                 >  2:559147/1‑151 (MQ=255)                                ccAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                                                 <  1:103310/151‑1 (MQ=255)                                   gtgtGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                                              <  1:787/151‑1 (MQ=255)                                      tgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTg                                                                                                           >  1:488673/1‑151 (MQ=255)                                       gATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTg                                                                                                           <  1:307813/150‑1 (MQ=255)                                       gATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTg                                                                                                           >  1:356029/1‑150 (MQ=255)                                          tctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATg                                                                                                        <  2:519127/150‑1 (MQ=255)                                             ttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCa                                                                                                      <  1:807996/149‑1 (MQ=255)                                                  aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAg                                                                                                   >  1:408617/1‑147 (MQ=255)                                                  aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                                                 >  2:654229/1‑149 (MQ=255)                                                    ctctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGc                                                                                              >  1:524398/1‑150 (MQ=255)                                                      ctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGc                                                                                              <  1:199962/148‑1 (MQ=255)                                                          cTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                                         >  1:787535/1‑149 (MQ=255)                                                            gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCa                                                                                        <  1:82094/148‑1 (MQ=255)                                                            gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCa                                                                                        <  2:340267/148‑1 (MQ=255)                                                               aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGtt                                                                                  <  1:329276/151‑1 (MQ=255)                                                                 tatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTAt                                                                                >  2:647827/1‑151 (MQ=255)                                                                  atTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTAt                                                                                <  1:530306/150‑1 (MQ=255)                                                                    tGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                                              <  2:606830/150‑1 (MQ=255)                                                                        gTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                                         <  1:231243/151‑1 (MQ=255)                                                                          aCATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                                       <  2:58858/151‑1 (MQ=255)                                                                            aTTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGCGAGCATTTCAGGCGTTATGCTTTCttat                                                                      >  1:683147/1‑150 (MQ=255)                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                                                  >  2:309496/1‑151 (MQ=255)                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                                                  >  1:754588/1‑151 (MQ=255)                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                                                  <  1:655879/151‑1 (MQ=255)                                                                                aaaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                                                  >  1:822482/1‑150 (MQ=255)                                                                                  aTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                               >  2:233880/1‑151 (MQ=255)                                                                                   tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                              >  2:264938/1‑151 (MQ=255)                                                                                        aaGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAata                                                         <  1:731754/151‑1 (MQ=255)                                                                                        aaGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAat                                                          >  1:173944/1‑150 (MQ=255)                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAgg                                                    >  2:624958/1‑151 (MQ=255)                                                                                                  tAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTc                                                >  2:659210/1‑150 (MQ=255)                                                                                                   aaCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                                               <  1:251416/150‑1 (MQ=255)                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAAt                                          <  1:818321/151‑1 (MQ=255)                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                                        >  2:505377/1‑151 (MQ=255)                                                                                                           tCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                                        >  1:639954/1‑149 (MQ=255)                                                                                                             attattAATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGagag                                    <  1:531295/151‑1 (MQ=255)                                                                                                               tattaATGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGtt                                  >  2:751457/1‑151 (MQ=255)                                                                                                                   aaTGACAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg                              >  1:683727/1‑151 (MQ=255)                                                                                                                        cAGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc                         >  2:99114/1‑151 (MQ=255)                                                                                                                         aGGGAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTcc                        >  1:260118/1‑151 (MQ=255)                                                                                                                           ggAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCAt                      <  1:471280/151‑1 (MQ=255)                                                                                                                            gAATCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCAtt                     <  2:8531/151‑1 (MQ=255)                                                                                                                              aTCCGTGCTCTCTTAGAACTTGGGTAGGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCt                   <  2:633076/151‑1 (MQ=255)                                                                                                                              aTCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCt                   >  1:495301/1‑151 (MQ=255)                                                                                                                              aTCCGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCt                   >  2:301657/1‑151 (MQ=255)                                                                                                                                ccGTGCTCTCTTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCtt                  <  1:478235/150‑1 (MQ=255)                                                                                                                                         ctTAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGat        >  1:584957/1‑151 (MQ=255)                                                                                                                                           tAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGat        <  2:324747/149‑1 (MQ=255)                                                                                                                                           tAGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGa         <  1:262832/148‑1 (MQ=255)                                                                                                                                            aGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGatat      <  2:663673/150‑1 (MQ=255)                                                                                                                                            aGAACTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGatat      <  2:721082/150‑1 (MQ=255)                                                                                                                                               aCTTGGGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCg  >  2:599765/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGACGCCTGATATCCCG  >  NC_000913/1216856‑1217148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑