Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,225,048	A→G	V105A (GTT→GCT)	minD ←	inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,225,048	0	A	G	100.0%	247.1 / NA	65	V105A (GTT→GCT)	minD	inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/33);  total (32/33)

ACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACG  >  NC_000913/1224913‑1225194                                                                                                                                        |                                                                                                                                                   aCTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCtt                                                                                                                                     >  1:449464/1‑151 (MQ=255)   tGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTc                                                                                                                                    >  2:290681/1‑150 (MQ=255)      ggagACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACgg                                                                                                                                >  2:23770/1‑151 (MQ=255)         gaCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTg                                                                                                                             <  1:612795/151‑1 (MQ=255)           cTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTg                                                                                                                             >  2:236776/1‑149 (MQ=255)            ttCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAgg                                                                                                                          >  1:272268/1‑151 (MQ=255)            ttCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAgg                                                                                                                          >  1:507361/1‑151 (MQ=255)             tCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAggg                                                                                                                         >  2:469370/1‑151 (MQ=255)             tCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAg                                                                                                                           <  1:635065/149‑1 (MQ=255)             tCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAg                                                                                                                           <  1:257199/149‑1 (MQ=255)                gggTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCAt                                                                                                                      <  1:10253/151‑1 (MQ=255)                     ggtggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGTGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCttt                                                                                                                  <  2:151569/150‑1 (MQ=255)                     ggtggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCttt                                                                                                                  <  1:343983/150‑1 (MQ=255)                      gtggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTAt                                                                                                                >  2:424593/1‑151 (MQ=255)                                tGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATTAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGttt                                                                                                       >  1:383006/1‑150 (MQ=255)                                 ggCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTg                                                                                                      <  2:651635/150‑1 (MQ=255)                                  gCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCg                                                                                                    <  1:333721/151‑1 (MQ=255)                                             aaaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGc                                                                                            >  1:749445/1‑148 (MQ=255)                                              aaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGa                                                                                         >  1:825129/1‑150 (MQ=255)                                                       gCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAt                                                                               <  1:275892/151‑1 (MQ=255)                                                        ccATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAtt                                                                              <  2:52405/151‑1 (MQ=255)                                                        ccATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAtt                                                                              <  1:307762/151‑1 (MQ=255)                                                          aTTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAtttt                                                                            <  2:536883/151‑1 (MQ=255)                                                           ttAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTc                                                                           >  1:181925/1‑151 (MQ=255)                                                            tAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTc                                                                           <  1:120295/150‑1 (MQ=255)                                                                     ggTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTa                                                                 <  2:742980/151‑1 (MQ=255)                                                                      gTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTAt                                                                <  1:496652/151‑1 (MQ=255)                                                                         tCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCGGAATATAGAGATTTTCAGTACGCTTATCt                                                              <  2:422793/150‑1 (MQ=255)                                                                         tCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCt                                                              <  1:511171/150‑1 (MQ=255)                                                                          cAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCttt                                                            >  2:581629/1‑151 (MQ=255)                                                                              cccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTtttatt                                                        <  1:90075/151‑1 (MQ=255)                                                                              cccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTttaatt                                                        >  2:321881/1‑151 (MQ=255)                                                                                  gCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAAc                                                     <  1:84804/150‑1 (MQ=255)                                                                                     ggggAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCt                                                 <  1:718551/151‑1 (MQ=255)                                                                                       ggAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGa                                               <  2:277218/151‑1 (MQ=255)                                                                                        gAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGAt                                              >  2:355012/1‑151 (MQ=255)                                                                                         aGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGAtt                                             >  1:388430/1‑151 (MQ=255)                                                                                          gTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGAttt                                            >  2:826885/1‑151 (MQ=255)                                                                                             acaAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGc                                         <  2:77320/151‑1 (MQ=255)                                                                                             acaAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGc                                         >  1:275352/1‑151 (MQ=255)                                                                                              caAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCg                                        >  2:142626/1‑151 (MQ=255)                                                                                                aaCGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGTGtt                                      >  2:22204/1‑151 (MQ=255)                                                                                                  cGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGc                                    >  2:459269/1‑151 (MQ=255)                                                                                                        aTTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGcc                              <  2:429425/151‑1 (MQ=255)                                                                                                        aTTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGcc                              >  1:607905/1‑151 (MQ=255)                                                                                                         ttCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGccc                             >  2:687321/1‑151 (MQ=255)                                                                                                         ttCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGccc                             >  1:781072/1‑151 (MQ=255)                                                                                                         ttCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGccc                             >  2:247635/1‑151 (MQ=255)                                                                                                           cAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCt                            <  1:343682/150‑1 (MQ=255)                                                                                                               aTCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATg                       <  2:526503/151‑1 (MQ=255)                                                                                                               aTCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATg                       <  2:454593/151‑1 (MQ=255)                                                                                                                  cATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACg                    <  1:67877/151‑1 (MQ=255)                                                                                                                    tCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGtt                  >  1:656558/1‑151 (MQ=255)                                                                                                                      gCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTg                 >  1:525971/1‑150 (MQ=255)                                                                                                                       cTTTCAGATCATCAAGAGCTTTGGCGCCCGCTTCACAGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCTGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGt                   <  2:653785/147‑1 (MQ=255)                                                                                                                        tttCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACg              >  2:751398/1‑151 (MQ=255)                                                                                                                              atcatcAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCg        >  2:243722/1‑151 (MQ=255)                                                                                                                              atcatcAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCg        <  1:236752/151‑1 (MQ=255)                                                                                                                               tcatcaAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt       >  1:558610/1‑151 (MQ=255)                                                                                                                                catcaAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt       >  1:128275/1‑150 (MQ=255)                                                                                                                                 atcaAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTa      >  2:360549/1‑150 (MQ=255)                                                                                                                                   caAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAAc   <  1:238521/151‑1 (MQ=255)                                                                                                                                   caAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAAc   <  2:132448/151‑1 (MQ=255)                                                                                                                                    aaGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACg  <  1:773497/151‑1 (MQ=255)                                                                                                                                    aaGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACg  <  2:810574/151‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                   ACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACG  >  NC_000913/1224913‑1225194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑