Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,237,152	T→C	A30A (GCA→GCG)	ycgB ←	SpoVR family stationary phase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,237,152	0	T	C	100.0%	169.4 / NA	49	A30A (GCA→GCG)	ycgB	SpoVR family stationary phase protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/21);  total (28/21)

ACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAT  >  NC_000913/1237017‑1237296                                                                                                                                        |                                                                                                                                                 aCCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATcc                                                                                                                                   >  2:68492/1‑151 (MQ=255)  ccGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATcc                                                                                                                                   <  2:639428/150‑1 (MQ=255)        gACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGt                                                                                                                            <  1:398540/151‑1 (MQ=255)          ccAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCg                                                                                                                          >  2:9660/1‑151 (MQ=255)          ccAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCg                                                                                                                          <  2:379852/151‑1 (MQ=255)           cAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGa                                                                                                                         <  2:576121/151‑1 (MQ=255)            aGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGaa                                                                                                                        <  2:791131/151‑1 (MQ=255)                    tAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGt                                                                                                                >  1:60228/1‑151 (MQ=255)                      gTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCg                                                                                                              <  1:68912/151‑1 (MQ=255)                          aTTGGCATGCCGACGCTGGAGTAGGCATACATCATTTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGAATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCCGATAAACATCAAGCAGGTCGAACGTCCAGTCGGGTc                                                                                                          >  1:559501/1‑151 (MQ=255)                               cATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCg                                                                                                     >  2:57/1‑151 (MQ=255)                                    cGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCa                                                                                                 >  2:501337/1‑150 (MQ=255)                                     gACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCaa                                                                                                >  1:67560/1‑150 (MQ=255)                                       cGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCaaa                                                                                               <  1:619387/149‑1 (MQ=255)                                           ggAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGtgtg                                                                                         >  2:111780/1‑151 (MQ=255)                                                aGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTc                                                                                    <  2:535494/151‑1 (MQ=255)                                                     tccatcATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTAt                                                                               >  1:661759/1‑151 (MQ=255)                                                      ccatcATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAACCATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTAtt                                                                              >  2:31381/1‑151 (MQ=255)                                                        atcatcTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTc                                                                             >  2:182129/1‑150 (MQ=255)                                                        atcatcTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTc                                                                             >  1:352053/1‑150 (MQ=255)                                                               gTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAat                                                                      <  1:231184/150‑1 (MQ=255)                                                                          aTCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGcc                                                           <  2:72794/150‑1 (MQ=255)                                                                             aCTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATAc                                                       <  2:490696/151‑1 (MQ=255)                                                                                tCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATAcgc                                                     <  1:120945/150‑1 (MQ=255)                                                                                  aaTCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACgcgc                                                   >  1:574368/1‑150 (MQ=255)                                                                                  aaTCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCa                                                  <  1:182106/151‑1 (MQ=255)                                                                                     cTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCt                                               >  2:291022/1‑151 (MQ=255)                                                                                          gCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAtt                                           <  2:27646/150‑1 (MQ=255)                                                                                           cGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAttgt                                         >  2:527583/1‑151 (MQ=255)                                                                                            gggTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAttgtt                                        >  2:583302/1‑151 (MQ=255)                                                                                             ggtaggtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCAttgttg                                       <  2:385853/151‑1 (MQ=255)                                                                                               taggtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTc                                     >  2:690155/1‑151 (MQ=255)                                                                                                aggtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCg                                    >  2:698496/1‑151 (MQ=255)                                                                                                  gtaTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCgg                                   >  1:678422/1‑150 (MQ=255)                                                                                                   taTCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGgcg                                 >  1:174443/1‑151 (MQ=255)                                                                                                             gTAGAGTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCtgtg                        >  1:409304/1‑150 (MQ=255)                                                                                                                 agTTTCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAg                   <  1:249576/151‑1 (MQ=255)                                                                                                                   tttCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGc                  <  2:220136/150‑1 (MQ=255)                                                                                                                    ttCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCa                 <  1:664845/150‑1 (MQ=255)                                                                                                                    ttCGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCAc                >  2:214283/1‑151 (MQ=255)                                                                                                                      cGCCACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCt              >  1:68290/1‑151 (MQ=255)                                                                                                                        ccACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTc             <  1:500435/150‑1 (MQ=255)                                                                                                                        ccACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCa            <  1:42490/151‑1 (MQ=255)                                                                                                                         cACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCa            >  2:343406/1‑150 (MQ=255)                                                                                                                         cACCCGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCAt           >  1:695544/1‑151 (MQ=255)                                                                                                                           cccGGTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCAttt         <  2:708025/151‑1 (MQ=255)                                                                                                                              ggTCTATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCa       >  1:71705/1‑150 (MQ=255)                                                                                                                                  tATCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAt  >  2:141160/1‑151 (MQ=255)                                                                                                                                   aTCTCCGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAt  >  2:265841/1‑150 (MQ=255)                                                                                                                                     ctccGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAt  >  1:139598/1‑148 (MQ=255)                                                                                                                                        |                                                                                                                                                 ACCGAATGACCAGTGCGGATAGTTAATTGGCATGCCGACGCTGGAGTAGGCATCCATCATCTGTTCTGAGGTTATCACTTCAATCTGGTGCGGGTAGGTATCCAGCCGGTAGAGTTTCGCCACCCGGTCTATCTCTGCCAGATAAACATCCAGCAGGTCGAACGTCCAGTCGGGTCCATCGCTCAAACGTGTGGTGTCCTTATTCATAGAATCGATCGTCGCCATACGCGCACCTCATTGTTGTCGGCGCTCTCTGTGTGGAGCACCTCATTTCAAGCAT  >  NC_000913/1237017‑1237296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑