Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,238,031	T→C	I154T (ATC→ACC)	dadA →	D‑amino acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,238,031	0	T	C	100.0%	146.0 / NA	42	I154T (ATC→ACC)	dadA	D‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/24);  total (18/24)

CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTGTCA  >  NC_000913/1237889‑1238172                                                                                                                                               |                                                                                                                                              cGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGc                                                                                                                                       <  1:171328/151‑1 (MQ=255)  ggATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCt                                                                                                                                      <  2:270730/151‑1 (MQ=255)   gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTg                                                                                                                                     <  2:556389/151‑1 (MQ=255)   gATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTg                                                                                                                                     >  2:48218/1‑151 (MQ=255)     tGGTGCGTCAGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCt                                                                                                                                      <  2:545711/148‑1 (MQ=255)              tGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCgg                                                                                                                          >  1:85612/1‑151 (MQ=255)               ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCgg                                                                                                                          <  2:829266/150‑1 (MQ=255)               ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGc                                                                                                                         <  2:306342/151‑1 (MQ=255)                   gAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTAc                                                                                                                     >  2:360859/1‑151 (MQ=255)                     aTACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCg                                                                                                                   >  2:321280/1‑151 (MQ=255)                             gTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGt                                                                                                                  >  2:859441/1‑144 (MQ=255)                             gTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGt                                                                                                                  <  1:859382/144‑1 (MQ=255)                               gATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAgctgc                                                                                                         >  1:199693/1‑151 (MQ=255)                                   gCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTgg                                                                                                      <  1:191238/150‑1 (MQ=255)                                    ccTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGaa                                                                                                    <  1:484761/151‑1 (MQ=255)                                             aTTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAgccgc                                                                                           <  2:290153/151‑1 (MQ=255)                                                gcgcgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCt                                                                                         <  2:160308/150‑1 (MQ=255)                                                       gAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGa                                                                                   >  1:178954/1‑149 (MQ=255)                                                            cAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAg                                                                            <  1:40846/151‑1 (MQ=255)                                                             aaTATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGc                                                                           <  1:473675/151‑1 (MQ=255)                                                              atatTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGcc                                                                          >  2:79384/1‑151 (MQ=255)                                                                atTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGccc                                                                         <  1:230731/150‑1 (MQ=255)                                                                      tATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTgg                                                                  >  1:568503/1‑151 (MQ=255)                                                                       aTGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGc                                                                 >  1:789375/1‑151 (MQ=255)                                                                               cGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTgg                                                         >  2:517385/1‑151 (MQ=255)                                                                                gTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGgc                                                        >  1:840256/1‑151 (MQ=255)                                                                                   aGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGca                                                     <  2:639244/151‑1 (MQ=255)                                                                                    gggTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGcac                                                    >  1:530462/1‑151 (MQ=255)                                                                                    gggTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGcac                                                    <  2:625925/151‑1 (MQ=255)                                                                                     ggTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGcaca                                                   <  1:847594/151‑1 (MQ=255)                                                                                        gggACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAAc                                                >  1:640583/1‑151 (MQ=255)                                                                                                 cAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGggcg                                       <  2:85635/151‑1 (MQ=255)                                                                                                   aCTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGggcggc                                     <  1:158797/151‑1 (MQ=255)                                                                                                        tCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGc                                 <  1:745751/150‑1 (MQ=255)                                                                                                                    aacaGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAatga                    <  1:81046/151‑1 (MQ=255)                                                                                                                     acaGTATGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAatgat                   <  1:699397/151‑1 (MQ=255)                                                                                                                          aTGAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAAc              >  2:19163/1‑151 (MQ=255)                                                                                                                            gAAAATGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCg            <  1:92200/151‑1 (MQ=255)                                                                                                                              aaaTGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCgg           >  2:764322/1‑150 (MQ=255)                                                                                                                               aaTGCGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCgg           >  1:339642/1‑149 (MQ=255)                                                                                                                                   cGACCCGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTg     >  1:531578/1‑151 (MQ=255)                                                                                                                                      cccGCGATACCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTGTCa  <  2:345219/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              CGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGACGGGCGGCCTGCAGTTACCCAATGATGAAACCGGAGACTGTCA  >  NC_000913/1237889‑1238172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑