Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,265,331 A→G D107G (GAC→GGC)  prfA → peptide chain release factor RF‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,265,3310AG98.2% 173.0 / NA 55D107G (GAC→GGC) prfApeptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (29/25);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTG  >  NC_000913/1265186‑1265474
                                                                                                                                                 |                                                                                                                                               
cAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAAc                                                                                                                                            >  2:544/1‑151 (MQ=255)
                 cGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGa                                                                                                                            >  2:681249/1‑150 (MQ=255)
                      aGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTcc                                                                                                                       >  2:614082/1‑150 (MQ=255)
                      aGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCg                                                                                                                      >  2:741622/1‑151 (MQ=255)
                      aGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCg                                                                                                                      >  1:585869/1‑151 (MQ=255)
                         tgatgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGc                                                                                                                   >  2:362142/1‑151 (MQ=255)
                           atgCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCg                                                                                                                 >  2:682507/1‑151 (MQ=255)
                             gCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCgg                                                                                                                >  1:707123/1‑150 (MQ=255)
                              cTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGa                                                                                                               >  2:837046/1‑150 (MQ=255)
                                cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGaa                                                                                                              <  2:424658/149‑1 (MQ=255)
                                cGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAAcc                                                                                                            >  2:117258/1‑151 (MQ=255)
                                   tgatCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACcggc                                                                                                         >  1:153761/1‑151 (MQ=255)
                                     atCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACcggcgg                                                                                                       <  1:725373/151‑1 (MQ=255)
                                        cTGCAATGCGAGAGATGACGCAGGATGAACTGCGAGAGCCTAAAGAAAACAGCGAGCAACCGGAACAGCAATTACAGGTTCTGCTACTGCCAAAAGATACTGATGACCAACGTAACGCCTTCCTCGAAGTCCGAGCCGGCACCggcggc                                                                                                      >  1:49666/1‑149 (MQ=255)
                                               gCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTGGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcg                                                                                             >  1:440320/1‑151 (MQ=255)
                                                cGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAgcgg                                                                                            <  1:184131/151‑1 (MQ=255)
                                                            cAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCgg                                                                                <  1:486775/151‑1 (MQ=255)
                                                                         gcgAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCg                                                                   >  2:10211/1‑151 (MQ=255)
                                                                           gAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGt                                                                  >  2:3151/1‑150 (MQ=255)
                                                                            aaGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTAt                                                                <  2:361098/151‑1 (MQ=255)
                                                                               cTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGt                                                              >  2:740319/1‑150 (MQ=255)
                                                                                 aaaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTAc                                                            >  2:649036/1‑150 (MQ=255)
                                                                                 aaaGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACa                                                           <  1:541596/151‑1 (MQ=255)
                                                                                   aGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc                                                                              <  1:217599/130‑1 (MQ=255)
                                                                                   aGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGc                                                                              >  2:217623/1‑130 (MQ=255)
                                                                                   aGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGc                                                         >  2:301352/1‑151 (MQ=255)
                                                                                       aaaaGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGtt                                                     >  1:778139/1‑151 (MQ=255)
                                                                                        aaaGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGtt                                                     >  1:111454/1‑150 (MQ=255)
                                                                                        aaaGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTa                                                    <  2:686064/151‑1 (MQ=255)
                                                                                         aaGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTAt                                                   <  2:122940/151‑1 (MQ=255)
                                                                                          aGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATg                                                  >  2:249076/1‑151 (MQ=255)
                                                                                             gAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCg                                               >  2:283580/1‑151 (MQ=255)
                                                                                                 aaCTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGc                                           >  2:14857/1‑151 (MQ=255)
                                                                                                       aaCAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCccgccg                                     <  2:398784/151‑1 (MQ=255)
                                                                                                             aaTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTACCGCCTCCCTCGAAGTCCGAGCCGGAACGGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTCCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTgg                                 <  2:131871/149‑1 (MQ=255)
                                                                                                             aaTTACAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCTTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCg                               <  2:843091/151‑1 (MQ=255)
                                                                                                                 aCAGGTTCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCggg                             >  1:285683/1‑149 (MQ=255)
                                                                                                                  cAGGTTATGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCgggggg                          >  1:642269/1‑148 (MQ=255)
                                                                                                                    gtttCTGTTACTGCCAAAAGATCCTGATGGCGAACGTATCGCCTCCCTCGAAGCCCGCGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGaa                        <  1:614579/149‑1 (MQ=255)
                                                                                                                      ttCTGTTACTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGaaa                       <  2:23224/150‑1 (MQ=255)
                                                                                                                        cTGTTCCTGCCAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAAt                      <  2:277673/149‑1 (MQ=255)
                                                                                                                           ttACTGCAAAAAGATCCTGAGGGCGAACGTACCGCCTTCCCCGAAGTCCGAGCCGAAACGGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCAt                   <  1:96440/149‑1 (MQ=255)
                                                                                                                           ttACTGCAAAAAGATCCGGAGGGCGAACGTAACCCTTTCCTCGAAGCCGGGGCCGGAACCGGGGGCGACGAGGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCAt                   <  1:490698/149‑1 (MQ=255)
                                                                                                                             actccaaaaaGATCCGGATGGCGAACGTACCGCCTTCCGCGAGGCCCGGGCCGGAACCGGCGGCGACGAAGCGGCGTTGTCCGCGGGCGATCTGTCCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAg                <  2:11942/144‑1 (MQ=255)
                                                                                                                                 ccAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGcc            >  2:850190/1‑150 (MQ=255)
                                                                                                                                 caaaaaGACCCGGATGGGGAACGTAACGCCTTCCTGGAAGTCGGAGCCGGAACCGGCGGCGACGAAGGGGCGCTGTTCGGGGGGGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAgcgc             <  1:648994/147‑1 (MQ=255)
                                                                                                                                  cAAAAGATCCTGATGGCGAACGTAACGCCTTCCTCGAGGTCCGAGCCGGACCCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCa           <  2:78850/150‑1 (MQ=255)
                                                                                                                                   aaaaGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGc         <  2:201379/151‑1 (MQ=255)
                                                                                                                                      aGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCg        >  2:443733/1‑149 (MQ=255)
                                                                                                                                      aGATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAg      <  2:546232/151‑1 (MQ=255)
                                                                                                                                       gATCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGAgg     >  1:368742/1‑151 (MQ=255)
                                                                                                                                       gATCCTGAGGGCGAACGTAACGCCTTCCTGGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTCCGCGGGCGATCTGTTCCGTAGGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAgg     <  2:497514/151‑1 (MQ=255)
                                                                                                                                        aTCCTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGGGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAggg    <  2:34921/151‑1 (MQ=255)
                                                                                                                                          ccTGAGGGCGAACGTACCGCCTTCCTCGAAGTCCGAGCCGGACCCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTg  <  1:14836/151‑1 (MQ=255)
                                                                                                                                           cTGATGGCGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGGAGAAATCATGAGCGCCAGCGAGGGTg  >  1:120258/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAGGAAGATATCGAAACCGCACAGATGATGCTCGATGATCCTGAAATGCGTGAGATGGCGCAGGATGAACTGCGCGAAGCTAAAGAAAAAAGCGAGCAACTGGAACAGCAATTACAGGTTCTGTTACTGCCAAAAGATCCTGATGACGAACGTAACGCCTTCCTCGAAGTCCGAGCCGGAACCGGCGGCGACGAAGCGGCGCTGTTCGCGGGCGATCTGTTCCGTATGTACAGCCGTTATGCCGAAGCCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTG  >  NC_000913/1265186‑1265474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑