Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,302,933 C→T A12V (GCG→GTG)  oppB → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,302,9330CT98.0% 181.2 / NA 49A12V (GCG→GTG) oppBoligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/23);  minor base G (1/0);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCC  >  NC_000913/1302793‑1303071
                                                                                                                                            |                                                                                                                                          
atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGAc                                                                                                                                   <  2:67954/150‑1 (MQ=255)
atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGAc                                                                                                                                   <  2:600363/150‑1 (MQ=255)
  gTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCt                                                                                                                                <  1:560895/151‑1 (MQ=255)
     cATTGTGAAGCACTAATGGGAATACGTGGTGCAGGAGTGTCATGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCAAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGGGATTCCGACGCtatt                                                                                                                             >  1:829248/1‑151 (MQ=255)
      aTTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtattt                                                                                                                            <  2:484674/151‑1 (MQ=255)
        tgtgAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCtattta                                                                                                                           <  1:804961/150‑1 (MQ=255)
           gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCt                                                                                                                       <  1:760759/151‑1 (MQ=255)
            aaGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCtt                                                                                                                      <  1:79390/151‑1 (MQ=255)
               cACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCttatt                                                                                                                   <  1:651450/151‑1 (MQ=255)
                aCTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCttatta                                                                                                                  <  2:796711/151‑1 (MQ=255)
                 cTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTAc                                                                                                                 >  1:162259/1‑151 (MQ=255)
                   aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACt                                                                                                                <  1:14800/150‑1 (MQ=255)
                      ggCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTAttt                                                                                                            <  2:599442/151‑1 (MQ=255)
                         aaTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGt                                                                                                         <  2:389592/151‑1 (MQ=255)
                             cGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCtt                                                                                                     <  2:44730/151‑1 (MQ=255)
                              gTGGGGCCGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGGAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCtt                                                                                                     >  1:607513/1‑150 (MQ=255)
                                  ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatga                                                                                                <  2:659843/151‑1 (MQ=255)
                                   gCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatgat                                                                                               <  2:136720/151‑1 (MQ=255)
                                   gCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatga                                                                                                >  1:827038/1‑150 (MQ=255)
                                    cAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatgatg                                                                                              >  1:59663/1‑151 (MQ=255)
                                    cAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatgatg                                                                                              >  2:150399/1‑151 (MQ=255)
                                     aGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATgc                                                                                             >  2:151215/1‑151 (MQ=255)
                                                gCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCg                                                                                  >  2:71479/1‑151 (MQ=255)
                                                   ccACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCgg                                                                                 <  1:799358/149‑1 (MQ=255)
                                                      cGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGc                                                                            >  1:650516/1‑151 (MQ=255)
                                                             tGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCtttt                                                                      >  2:630251/1‑150 (MQ=255)
                                                             tGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCtttt                                                                      >  1:487899/1‑150 (MQ=255)
                                                                 ttttATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCgg                                                                 >  2:53032/1‑151 (MQ=255)
                                                                    tATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCg                                                               >  1:636113/1‑150 (MQ=255)
                                                                    tATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCg                                                               <  2:739119/150‑1 (MQ=255)
                                                                         cATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGt                                                          >  2:278207/1‑150 (MQ=255)
                                                                               aGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTAc                                                   >  1:428711/1‑151 (MQ=255)
                                                                                gAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTAcc                                                  >  1:70263/1‑151 (MQ=255)
                                                                                 aaGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTAcc                                                  >  1:843411/1‑150 (MQ=255)
                                                                                 aaGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTAcc                                                  >  1:445029/1‑150 (MQ=255)
                                                                                   ggCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGcc                                               >  1:151055/1‑151 (MQ=255)
                                                                                         ggCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGt                                         >  1:200635/1‑151 (MQ=255)
                                                                                              gAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATgg                                    <  1:131185/151‑1 (MQ=255)
                                                                                                aGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGCCGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCCCCGGGAAGCCCTTGTACCGGCGAACGTTCTTTACCGCCAGAAGTGATGGc                                   <  1:781884/150‑1 (MQ=255)
                                                                                                     gggCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAatat                             >  1:533311/1‑151 (MQ=255)
                                                                                                      ggCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAatat                             >  2:671503/1‑150 (MQ=255)
                                                                                                         aaTGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGa                          >  2:537148/1‑150 (MQ=255)
                                                                                                         aaTGTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGa                          >  1:61951/1‑150 (MQ=255)
                                                                                                            gTTAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGc                       <  1:741515/150‑1 (MQ=255)
                                                                                                             ttAAAATTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCg                      <  2:241693/150‑1 (MQ=255)
                                                                                                                 aaTTTATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAat                  >  2:166083/1‑150 (MQ=255)
                                                                                                                     tATTCTACGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATatca              <  2:608410/150‑1 (MQ=255)
                                                                                                                           aCGTCGCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTaa        <  1:30309/150‑1 (MQ=255)
                                                                                                                                gCTGTCTGGAAGTGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATcc  >  2:821635/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                          
ATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCC  >  NC_000913/1302793‑1303071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑