| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,318,202 | T→C | T72A (ACG→GCG) | trpB ← | tryptophan synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,318,202 | 0 | T | C | 98.3% | 197.0 / NA | 58 | T72A (ACG→GCG) | trpB | tryptophan synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (27/30); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGACGCCACGCCATGCTGACCGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGC > NC_000913/1318058‑1318346 | ccGACGCCACGCCATGCTGACCGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGtt > 2:305945/1‑151 (MQ=255) actccacgccaTGCTGACCGGCCCCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTTGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCg < 2:213798/147‑1 (MQ=255) acgccaTGCTGACCGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCgg < 1:95200/151‑1 (MQ=255) gCTGACCGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAAt < 2:443281/151‑1 (MQ=255) gACCGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGt < 1:698255/150‑1 (MQ=255) cGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTg < 2:391754/151‑1 (MQ=255) cACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGc > 1:613604/1‑150 (MQ=255) ggTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTg > 1:803536/1‑151 (MQ=255) ggCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAgc > 2:490744/1‑151 (MQ=255) gatgatTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGcgcg > 2:515855/1‑151 (MQ=255) gatTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGtt < 2:222624/151‑1 (MQ=255) atTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTg > 2:739641/1‑151 (MQ=255) ttCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTgg > 2:451228/1‑150 (MQ=255) cGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGAc < 2:332091/150‑1 (MQ=255) cGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACg < 1:413275/151‑1 (MQ=255) cGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACg > 1:297780/1‑151 (MQ=255) cGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTATTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACg > 2:827268/1‑151 (MQ=255) gggtttACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGc > 1:544667/4‑151 (MQ=255) tttACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGccc < 2:389907/150‑1 (MQ=255) ttACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCgg > 2:718745/1‑151 (MQ=255) tACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCgg < 1:222615/150‑1 (MQ=255) aCCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGc > 1:117538/1‑150 (MQ=255) tCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTc > 2:314110/1‑149 (MQ=255) agacAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTcag > 1:225368/4‑150 (MQ=255) gCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTcagca < 2:228428/151‑1 (MQ=255) aGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGt > 2:495864/1‑151 (MQ=255) aaCGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGt < 2:240959/151‑1 (MQ=255) aCGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGtt < 2:694961/151‑1 (MQ=255) cTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAAc > 2:234816/1‑151 (MQ=255) tGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAAc > 1:841393/1‑150 (MQ=255) gCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACt < 1:228052/150‑1 (MQ=255) gCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTg < 1:110955/151‑1 (MQ=255) cACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTg > 2:435879/1‑150 (MQ=255) cACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGa < 1:673931/151‑1 (MQ=255) ggTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAAtt < 1:525299/150‑1 (MQ=255) ggTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTc > 1:800564/1‑151 (MQ=255) ggTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTc > 1:799323/1‑151 (MQ=255) tAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAg < 1:809214/150‑1 (MQ=255) aGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAgg < 2:385277/150‑1 (MQ=255) aGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGa < 1:728348/151‑1 (MQ=255) aGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGa < 2:762554/151‑1 (MQ=255) ttttATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATc > 2:152078/1‑151 (MQ=255) tttATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATc < 1:218380/150‑1 (MQ=255) aTGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCtttt < 2:123628/151‑1 (MQ=255) aTGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCtttt < 1:41898/151‑1 (MQ=255) tGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCttttt > 2:486606/1‑151 (MQ=255) gcgcgcCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTg > 2:76759/1‑151 (MQ=255) cgcgcCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTgc < 2:486596/151‑1 (MQ=255) gccgccGTGCAGCAAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTgcgc > 1:66668/1‑150 (MQ=255) cagcaAATCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTCGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACaaaa < 2:94037/151‑1 (MQ=255) aaaTCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGctt < 2:667248/151‑1 (MQ=255) aaaTCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGctt > 2:38627/1‑151 (MQ=255) aaaTCTTCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGctt > 2:249375/1‑151 (MQ=255) ttCACGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTcc > 2:238682/1‑151 (MQ=255) cGCTTGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCt < 1:63272/151‑1 (MQ=255) tGAGATACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTgg < 2:99570/149‑1 (MQ=255) gaTACAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCa < 2:636345/150‑1 (MQ=255) cAGCGCGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGc > 2:844073/1‑150 (MQ=255) | CCGACGCCACGCCATGCTGACCGGCACCGGTTTCGGCGATGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGC > NC_000913/1318058‑1318346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |