Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,333,046	A→G	T667A (ACG→GCG)	topA →	DNA topoisomerase I, omega subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,333,046	0	A	G	100.0%	204.7 / NA	58	T667A (ACG→GCG)	topA	DNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/33);  total (25/33)

TCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCT  >  NC_000913/1332905‑1333193                                                                                                                                              |                                                                                                                                                    tCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGcggcgcggcg                                                                                                                                                <  1:13462/147‑1 (MQ=255) tCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATg                                                                                                                                             <  2:401147/150‑1 (MQ=255)  cTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATg                                                                                                                                             <  1:168145/149‑1 (MQ=255)    ggCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGAc                                                                                                                                          >  2:284866/1‑150 (MQ=255)     gCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGAc                                                                                                                                          >  1:595486/1‑149 (MQ=255)      cTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGc                                                                                                                                       >  1:730503/1‑151 (MQ=255)         tGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTAt                                                                                                                                    >  1:468726/1‑151 (MQ=255)           cgcTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTAtct                                                                                                                                  <  2:433/151‑1 (MQ=255)              tGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCAt                                                                                                                               >  1:206420/1‑151 (MQ=255)               gccgccGAAAGAGCGTTGCAAAGCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCGCGAAATGCGGCGCGGCGATGGACAGCTATCTCAt                                                                                                                               <  2:253523/150‑1 (MQ=255)               gccgccGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCAt                                                                                                                               <  2:194204/150‑1 (MQ=255)               gccgccGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATc                                                                                                                              <  1:628023/151‑1 (MQ=255)                  gccgAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGAt                                                                                                                           >  2:740342/1‑151 (MQ=255)                     gAAAGAGCGTTGCAAAACCATCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCg                                                                                                                        <  2:134045/151‑1 (MQ=255)                       aaGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGaa                                                                                                                      <  2:695630/151‑1 (MQ=255)                            cGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGt                                                                                                                  >  1:803770/1‑150 (MQ=255)                                gCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGt                                                                                                              <  1:721462/150‑1 (MQ=255)                                   aaaCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGc                                                                                                           >  2:24464/1‑150 (MQ=255)                                   aaaCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCa                                                                                                          <  1:313751/151‑1 (MQ=255)                                    aaCCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCAt                                                                                                         >  2:783939/1‑151 (MQ=255)                                     accaccATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATg                                                                                                        >  2:609821/1‑151 (MQ=255)                                       caccaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTc                                                                                                      <  1:661820/151‑1 (MQ=255)                                        accaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCt                                                                                                     <  2:554156/151‑1 (MQ=255)                                         ccaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                                                                    <  1:73405/151‑1 (MQ=255)                                         ccaTTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCtg                                                                                                    <  2:344662/151‑1 (MQ=255)                                             tAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTgg                                                                                                 <  1:247908/150‑1 (MQ=255)                                                ccTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGtaat                                                                                             <  2:208445/151‑1 (MQ=255)                                                     tGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAccc                                                                                        >  1:180186/1‑151 (MQ=255)                                                        cGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAAc                                                                                     >  2:665764/1‑151 (MQ=255)                                                          gAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCt                                                                                   <  1:522285/151‑1 (MQ=255)                                                          gAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCt                                                                                   <  2:18674/151‑1 (MQ=255)                                                           aaaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTg                                                                                  <  2:706263/151‑1 (MQ=255)                                                           aaaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTg                                                                                  >  1:407745/1‑151 (MQ=255)                                                            aaaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGc                                                                                 <  2:733729/151‑1 (MQ=255)                                                             aaCGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCg                                                                                <  2:683317/151‑1 (MQ=255)                                                               cGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGAc                                                                              >  1:661668/1‑151 (MQ=255)                                                                gAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACg                                                                             >  2:639096/1‑151 (MQ=255)                                                                  aGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGt                                                                           <  1:575242/151‑1 (MQ=255)                                                                    tGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGtt                                                                          >  2:562190/1‑150 (MQ=255)                                                                      cTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTAc                                                                        <  2:104306/150‑1 (MQ=255)                                                                      cTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACg                                                                       >  1:799846/1‑151 (MQ=255)                                                                      cTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACg                                                                       >  1:280500/1‑151 (MQ=255)                                                                        gAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACga                                                                      <  1:562152/150‑1 (MQ=255)                                                                                 ggAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAaga                                                             <  1:760964/150‑1 (MQ=255)                                                                                  gAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAgg                                                           <  2:803826/151‑1 (MQ=255)                                                                                  gAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAgg                                                           >  2:330185/1‑151 (MQ=255)                                                                                       cGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGaa                                                      <  2:177405/151‑1 (MQ=255)                                                                                         aaGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAAtt                                                    >  2:195312/1‑151 (MQ=255)                                                                                          aGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTc                                                   >  2:620141/1‑151 (MQ=255)                                                                                                 gAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAtt                                             >  2:748771/1‑150 (MQ=255)                                                                                                 gAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCAtt                                             <  1:718787/150‑1 (MQ=255)                                                                                                   acccAACGCGCTGCGCGCAAAACGTGGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAAACCAACCTGCGACAGTTACGAGATCGAAGAGGGCGAATTCCGCATTaaa                                          <  1:857170/149‑1 (MQ=255)                                                                                                   aaCCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTaaa                                          <  1:726847/151‑1 (MQ=255)                                                                                                   aaCCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTaa                                           >  1:185596/1‑150 (MQ=255)                                                                                                    aCCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTaa                                           >  2:427086/1‑149 (MQ=255)                                                                                                         cgcgCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTAt                                    <  1:734681/151‑1 (MQ=255)                                                                                                                     aaaaCGTCGTTGCCCGAAATGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATc                        >  2:387144/1‑151 (MQ=255)                                                                                                                                      aaTGCGGCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAAtg       <  2:40111/151‑1 (MQ=255)                                                                                                                                         gcggcgcggcGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAAtgtg     <  2:646337/150‑1 (MQ=255)                                                                                                                                         gcggcgcggcGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAAtgtg     <  2:186016/150‑1 (MQ=255)                                                                                                                                           ggCGCGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGct  <  2:44903/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    TCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCT  >  NC_000913/1332905‑1333193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑