Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,703	T→C	G291G (GGT→GGC)	acnA →	aconitate hydratase 1; aconitase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,703	0	T	C	100.0%	187.0 / NA	51	G291G (GGT→GGC)	acnA	aconitate hydratase 1; aconitase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/29);  total (22/29)

TTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGT  >  NC_000913/1336555‑1336849                                                                                                                                                     |                                                                                                                                                   tttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCt                                                                                                                                                  >  1:512557/1‑151 (MQ=255)  ttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCt                                                                                                                                                  <  1:342251/150‑1 (MQ=255)   tCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTgg                                                                                                                                                >  2:218487/1‑151 (MQ=255)     cATGCTTATCCCGGATGTAGTGGGCTTCAACCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTgg                                                                                                                                                <  1:667600/149‑1 (MQ=255)          ttATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGGTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACt                                                                                                                                         >  1:701178/1‑151 (MQ=255)           tATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACt                                                                                                                                         >  2:77530/1‑150 (MQ=255)              cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCg                                                                                                                                     >  2:490979/1‑151 (MQ=255)               ccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACACGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGt                                                                                                                                    >  2:592529/1‑151 (MQ=255)                   aTGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTgg                                                                                                                                 <  2:72712/150‑1 (MQ=255)                     gTAGTGGGCTTCAATCTGACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGc                                                                                                                                <  1:473172/149‑1 (MQ=255)                           ggCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATcgcg                                                                                                                        <  1:567773/151‑1 (MQ=255)                                        ccGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGcc                                                                                                             <  2:754088/149‑1 (MQ=255)                                        ccGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGcc                                                                                                             <  2:43687/149‑1 (MQ=255)                                         cGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                          <  2:107256/151‑1 (MQ=255)                                          ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                          >  1:771276/1‑150 (MQ=255)                                                ttACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCg                                                                                                    <  1:44483/150‑1 (MQ=255)                                                 tACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGcc                                                                                                  >  1:97364/1‑151 (MQ=255)                                                  aCGTGAAGCTATAACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACAGGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCGCCATTGCCAATATGTCGcc                                                                                                  <  1:82347/150‑1 (MQ=255)                                                        aGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                           <  2:584449/151‑1 (MQ=255)                                                        aGGCATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                           >  1:214965/1‑151 (MQ=255)                                                         ggTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATg                                                                                          >  1:389020/1‑151 (MQ=255)                                                           tATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGt                                                                                        >  2:324627/1‑151 (MQ=255)                                                            aTTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTg                                                                                       <  2:96424/151‑1 (MQ=255)                                                             ttACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGt                                                                                        <  1:770545/149‑1 (MQ=255)                                                              tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                                                                                                        >  1:416160/1‑132 (MQ=255)                                                              tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAatat                                                                                                        <  2:416193/132‑1 (MQ=255)                                                                   ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtg                                                                                <  1:848899/151‑1 (MQ=255)                                                                         aCCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGctt                                                                          <  1:450802/151‑1 (MQ=255)                                                                            tGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttctt                                                                       >  2:331086/1‑151 (MQ=255)                                                                               ttCTCAATGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCATCTTccc                                                                    >  1:821262/1‑151 (MQ=255)                                                                                   cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAAt                                                                 <  2:195357/150‑1 (MQ=255)                                                                                       gTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGa                                                              <  1:394544/149‑1 (MQ=255)                                                                                          aCCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCt                                                          >  1:43906/1‑150 (MQ=255)                                                                                          aCCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTg                                                         <  1:166432/151‑1 (MQ=255)                                                                                                  gctgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCt                                                  <  1:46890/150‑1 (MQ=255)                                                                                                  gctgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTc                                                 <  2:656317/151‑1 (MQ=255)                                                                                                     gcgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGa                                               <  1:639499/150‑1 (MQ=255)                                                                                                      cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAtt                                             <  1:405430/151‑1 (MQ=255)                                                                                                               ggCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGtt                                    <  2:177028/151‑1 (MQ=255)                                                                                                                       ggggAAATTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCggg                            >  1:8808/1‑151 (MQ=255)                                                                                                                           aaaTTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCa                        <  2:674391/151‑1 (MQ=255)                                                                                                                            aaTTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                         >  1:159552/1‑149 (MQ=255)                                                                                                                             aTTCGTCGAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGc                      >  2:811693/1‑151 (MQ=255)                                                                                                                                 gtcgAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAg                  <  1:295360/151‑1 (MQ=255)                                                                                                                                   cgAATTTTATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa                    >  1:578041/1‑147 (MQ=255)                                                                                                                                        tttCTGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGTCAATATGTCTCCAGTTTATGGTGCGACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGCAGATCAGGt           >  1:550341/1‑151 (MQ=255)                                                                                                                                         ttATGGTGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTc          >  2:122167/1‑151 (MQ=255)                                                                                                                                               tGATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTg    >  1:278210/1‑151 (MQ=255)                                                                                                                                                gATGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTg    <  2:295336/150‑1 (MQ=255)                                                                                                                                                 aTGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGt  <  2:46674/151‑1 (MQ=255)                                                                                                                                                 aTGGCCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGt  <  2:404275/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGT  >  NC_000913/1336555‑1336849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑