Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,362	T→C	N47N (AAT→AAC)	ycjO →	putative sugar ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,362	0	T	C	100.0%	228.7 / NA	65	N47N (AAT→AAC)	ycjO	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/28);  total (37/28)

TCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCG  >  NC_000913/1373216‑1373506                                                                                                                                                   |                                                                                                                                                 tCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAAcccc                                                                                                                                               >  1:214539/1‑150 (MQ=255)   cGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAAc                                                                                                                                            >  2:619870/1‑151 (MQ=255)    gCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAAc                                                                                                                                            >  1:669263/1‑150 (MQ=255)      aaTGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATc                                                                                                                                         >  1:644463/1‑151 (MQ=255)      aaTGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATc                                                                                                                                         >  1:46413/1‑151 (MQ=255)          aaCAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGt                                                                                                                                     <  2:285111/151‑1 (MQ=255)               gCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACg                                                                                                                                >  1:459708/1‑151 (MQ=255)                       cAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTgg                                                                                                                        <  1:565935/151‑1 (MQ=255)                             gTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAg                                                                                                                  <  1:375540/151‑1 (MQ=255)                                ccGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCaa                                                                                                               <  1:98191/151‑1 (MQ=255)                                ccGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCaa                                                                                                               <  1:816197/151‑1 (MQ=255)                                  gATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACt                                                                                                             >  2:634102/1‑151 (MQ=255)                                  gATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACt                                                                                                             <  1:448498/151‑1 (MQ=255)                                         ccTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCg                                                                                                      >  1:484765/1‑151 (MQ=255)                                           tttGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGt                                                                                                     >  2:173798/1‑150 (MQ=255)                                            ttGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTAt                                                                                                   >  2:501898/1‑151 (MQ=255)                                             tGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTCTGTGCGTATc                                                                                                  >  2:41225/1‑151 (MQ=255)                                               cgcTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCct                                                                                                <  1:186252/151‑1 (MQ=255)                                               cgcTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTATGTTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATcc                                                                                                 <  1:663501/150‑1 (MQ=255)                                                 ctgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCctct                                                                                              >  1:264570/1‑151 (MQ=255)                                                  tgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTTGGGGGAGCAACCATGGGCGTATCCtctc                                                                                             >  1:478175/1‑151 (MQ=255)                                                  tgctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCtctc                                                                                             >  1:657827/1‑151 (MQ=255)                                                   gctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTcc                                                                                            <  2:846210/151‑1 (MQ=255)                                                   gctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGGGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCCACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTcc                                                                                            >  1:94674/1‑151 (MQ=255)                                                    ctgctTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGGCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCg                                                                                           >  1:560115/1‑151 (MQ=255)                                                          ctcGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATc                                                                                        <  1:17786/148‑1 (MQ=255)                                                          ctcGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCg                                                                                     >  1:492713/1‑151 (MQ=255)                                                            cgcgCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACTTCGAGTCAACTTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCgg                                                                                    <  1:574079/150‑1 (MQ=255)                                                             gcgcCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCGCCGATCCCGGc                                                                                   <  1:14730/150‑1 (MQ=255)                                                                    gCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGc                                                                            >  1:183054/1‑150 (MQ=255)                                                                        attaCTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTc                                                                        <  1:737101/150‑1 (MQ=255)                                                                          taCTGCTGGGCGGTCTGGTGGCGTGGCCGACGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCt                                                                     >  1:294044/1‑151 (MQ=255)                                                                             tgctgGGCGGTCTGGTGGCGTCGCCGATGGTGTCGCATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGCGAGCAACTCTGTGCGTATCCTCTCCGCTCCCGGCTTCTGGCATTCGCtgtg                                                                  >  1:706923/1‑151 (MQ=255)                                                                              gctgGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCtgtg                                                                  <  2:744376/150‑1 (MQ=255)                                                                                    cGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACg                                                           <  2:484801/151‑1 (MQ=255)                                                                                          ggtggCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTgg                                                     >  2:831221/1‑151 (MQ=255)                                                                                           gtggcgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGt                                                    <  1:346722/151‑1 (MQ=255)                                                                                             ggcgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGtat                                                  <  2:142724/151‑1 (MQ=255)                                                                                              gcgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGtata                                                 <  2:167600/151‑1 (MQ=255)                                                                                               cgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCAGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATAc                                                >  2:686298/1‑151 (MQ=255)                                                                                                 tggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACcg                                              >  2:761820/1‑151 (MQ=255)                                                                                                    ccGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGtata                                                 <  1:467155/145‑1 (MQ=255)                                                                                                    ccGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGtata                                                 >  2:467191/1‑145 (MQ=255)                                                                                                      gATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtg                                         >  2:854353/1‑151 (MQ=255)                                                                                                      gATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtg                                         >  1:718896/1‑151 (MQ=255)                                                                                                       aTGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtgg                                        <  1:132747/151‑1 (MQ=255)                                                                                                          gtgtCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtggtgg                                     <  2:199085/151‑1 (MQ=255)                                                                                                           tgtCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtggtggt                                    >  1:607553/1‑151 (MQ=255)                                                                                                             tCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCtggtggtgg                                  >  2:55704/1‑151 (MQ=255)                                                                                                               gAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCg                                >  2:230205/1‑151 (MQ=255)                                                                                                                           cAGTTTTTTACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGt                     <  2:490873/150‑1 (MQ=255)                                                                                                                               tttttACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTc                 <  1:550334/150‑1 (MQ=255)                                                                                                                               tttttACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTc                 <  2:681735/150‑1 (MQ=255)                                                                                                                                  ttACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGc             >  1:309009/1‑151 (MQ=255)                                                                                                                                   tACGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGc             <  1:478878/150‑1 (MQ=255)                                                                                                                                    aCGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTg           >  2:329813/1‑151 (MQ=255)                                                                                                                                    aCGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTg           >  2:146933/1‑151 (MQ=255)                                                                                                                                     cGTCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTgg          >  1:556133/1‑151 (MQ=255)                                                                                                                                       tCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCg        >  1:225302/1‑151 (MQ=255)                                                                                                                                       tCTGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCg        >  2:5138/1‑151 (MQ=255)                                                                                                                                         tGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATTTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCgg       >  1:313554/1‑150 (MQ=255)                                                                                                                                         tGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTc                                                                        <  2:522750/85‑1 (MQ=255)                                                                                                                                         tGCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTc                                                                        >  1:522712/1‑85 (MQ=255)                                                                                                                                          gCCGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTg     <  2:31352/151‑1 (MQ=255)                                                                                                                                            cGCTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGc   <  2:214563/151‑1 (MQ=255)                                                                                                                                              cTCAACCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCg  >  2:275179/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TCCGCAATGAACAGGCTTTTTTCAGGTCGTTCCGATATGCCCTTTGCGCTGCTGCTTCTCGCGCCCAGCTTATTACTGCTGGGCGGTCTGGTGGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCG  >  NC_000913/1373216‑1373506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑