Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,994,461	(T)8→7	pseudogene (632/633 nt)	ygeK ←	pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,994,454	0	T	.	100.0%	292.5 / NA	60	intergenic (+13/+6)	pbl/ygeK	pseudogene, peptidoglycan‑binding enzyme family/pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (34/26);  total (34/26)

ACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTT  >  NC_000913/2994305‑2994600                                                                                                                                                      |                                                                                                                                                   aCGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtt                                                                                                                                                   <  2:306564/151‑1 (MQ=255)     ggCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtttttt                                                                                                                                               <  1:682856/151‑1 (MQ=255)                 ggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCaa                                                                                                                                  <  1:725677/151‑1 (MQ=255)                      tataATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAacac                                                                                                                              <  2:197125/150‑1 (MQ=255)                             cGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGt                                                                                                                      >  2:749751/1‑151 (MQ=255)                                 ttttGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTaaa                                                                                                                   <  1:378217/150‑1 (MQ=255)                                     gCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAGGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGt                                                                                                              >  2:582007/1‑151 (MQ=255)                                     gCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGt                                                                                                              >  1:547307/1‑151 (MQ=255)                                      cAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGtt                                                                                                             <  2:320852/151‑1 (MQ=255)                                      cAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGtt                                                                                                             >  2:663847/1‑151 (MQ=255)                                       aaTGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGttt                                                                                                            <  2:759247/151‑1 (MQ=255)                                         tGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTg                                                                                                          >  2:644358/1‑151 (MQ=255)                                          ggATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTg                                                                                                          <  1:280356/150‑1 (MQ=255)                                           gATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAGCTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGAt                                                                                                        >  2:281188/1‑151 (MQ=255)                                                ccAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt                                                                                                   >  1:702482/1‑151 (MQ=255)                                                    aaaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGAt                                                                                               <  2:539329/151‑1 (MQ=255)                                                    aaaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGAt                                                                                               >  1:252836/1‑151 (MQ=255)                                                    aaaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGAt                                                                                               <  2:75597/151‑1 (MQ=255)                                                           gaCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtg                                                                                        >  1:308055/1‑151 (MQ=255)                                                           gaCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtg                                                                                        >  2:443709/1‑151 (MQ=255)                                                           gaCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtg                                                                                        <  1:633727/151‑1 (MQ=255)                                                            aCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtgt                                                                                       >  2:711239/1‑151 (MQ=255)                                                             cAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtgtg                                                                                      >  2:285080/1‑151 (MQ=255)                                                             cAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtgtg                                                                                      <  1:98057/151‑1 (MQ=255)                                                                cAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAAc                                                                                   <  1:727066/151‑1 (MQ=255)                                                                 aaTATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAAc                                                                                   >  2:372723/1‑150 (MQ=255)                                                                 aaTATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACa                                                                                  <  2:686049/151‑1 (MQ=255)                                                                      gCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTa                                                                              <  2:708246/150‑1 (MQ=255)                                                                           aaaaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCTTC‑TTTTTTTATATAGTGCAAACACCCATACGT‑AAGCTGGTTTGATTCACTTCATTACCCTGGGAACATCTAAtttttt                                                                       >  1:217970/1‑151 (MQ=255)                                                                            aaaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttctt                                                                       <  2:148022/151‑1 (MQ=255)                                                                             aaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttcttc                                                                      >  1:127559/1‑151 (MQ=255)                                                                              atatatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttcttc                                                                      >  1:553589/1‑150 (MQ=255)                                                                              atatatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCa                                                                     <  2:62097/151‑1 (MQ=255)                                                                                atatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg                                                                   <  1:309026/151‑1 (MQ=255)                                                                                 tatatTGTATATACCAGGCTTAATGAACTATACAAGCGTAATGCTGTAGCAAAATGAGCCTGACGCCTC‑ATTTTTTATATAGTGCAAACAACCAGACGTAAAGCTGGTTTGATCACCTCGATACCACTGTGAACATCCaaaatattcagg                                                                   <  1:309043/150‑11 (MQ=255)                                                                                 tatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg                                                                   >  1:594315/1‑150 (MQ=255)                                                                                   tatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat                                                                 >  2:493453/1‑150 (MQ=255)                                                                                   tatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat                                                                 >  1:666579/1‑150 (MQ=255)                                                                                    atTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGata                                                                <  2:706050/150‑1 (MQ=255)                                                                                      tGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt                                                              <  2:720934/150‑1 (MQ=255)                                                                                          tataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTc                                                         >  2:644839/1‑151 (MQ=255)                                                                                           ataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCt                                                        >  1:699196/1‑151 (MQ=255)                                                                                              ccAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATg                                                     >  2:494239/1‑151 (MQ=255)                                                                                                   cTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGttt                                                >  2:264946/1‑151 (MQ=255)                                                                                                    ttAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTc                                               >  2:170647/1‑151 (MQ=255)                                                                                                     tAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCg                                              >  2:64556/1‑151 (MQ=255)                                                                                                         gAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTg                                          >  1:228964/1‑151 (MQ=255)                                                                                                                aCAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCt                                   >  1:711535/1‑151 (MQ=255)                                                                                                                 cAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTg                                  <  1:85153/151‑1 (MQ=255)                                                                                                                 cAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTg                                  <  1:732484/151‑1 (MQ=255)                                                                                                                  aaTCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGc                                 >  1:458018/1‑151 (MQ=255)                                                                                                                      gTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTa                              >  2:695090/1‑150 (MQ=255)                                                                                                                           gCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCa                        <  2:324474/151‑1 (MQ=255)                                                                                                                              tCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCa                        >  2:606266/1‑148 (MQ=255)                                                                                                                                    aaaTGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAg               <  1:611599/151‑1 (MQ=255)                                                                                                                                      aTGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCg             >  1:45270/1‑151 (MQ=255)                                                                                                                                      aTGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCg             >  2:208893/1‑151 (MQ=255)                                                                                                                                       tGAGCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCg             >  1:124424/1‑150 (MQ=255)                                                                                                                                          gCCTAAAGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGAttt         <  1:447166/151‑1 (MQ=255)                                                                                                                                              aaaGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCttt     >  1:353582/1‑151 (MQ=255)                                                                                                                                               aaGCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCtttg    <  2:697855/151‑1 (MQ=255)                                                                                                                                                 gCCTC‑TTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCtttgtt  <  1:724642/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   ACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTT  >  NC_000913/2994305‑2994600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑