| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 483,728 | Δ1 bp | coding (676/3150 nt) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 483,726 | 0 | T | . | 89.7% | 97.8 / 6.4 | 32 | coding (678/3150 nt) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (0/2); major base . (16/10); minor base C (0/3); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCCTTTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGT > NC_000913/483579‑483864 | gTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCCtt > 2:46684/1‑149 (MQ=255) tcACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACcggcgg > 1:750127/1‑151 (MQ=255) caccacCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCggcggc < 1:582783/151‑1 (MQ=255) ccaccCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGt < 1:708539/151‑1 (MQ=255) ccAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTacc‑a < 1:169144/151‑1 (MQ=255) aaTCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCt > 1:530759/1‑151 (MQ=255) cgACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGAccc < 1:545916/148‑1 (MQ=255) gACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACccgccg > 2:312589/1‑151 (MQ=255) aCGTCACGCAGCAGCACGCGGGAACCATCCTGATTCGCTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGGCAGACGCGTCTGAGCAATAATAGAGGGGTT‑AAGCTGTTGGCC‑TTCTCCGGCGGCGTACC‑ACCGAGCTg < 1:686741/142‑1 (MQ=255) gggggAACCATCCTGATACCCTGCCAGCAGGATTTGGCCGCACTCTAAAGTAGGGGACAGGCGCGTCGGAGCAATAGTGGGGGCTTT‑AGGGTGTTGG‑CGTTCACCGGCGGC‑TACCGACCGAGCACACCCGCCGCAACCTGGGCGTTCTgc < 1:85361/148‑1 (MQ=255) ggggaaaCCACCCTGATTCTCTTTCAGCAGGATTTTGCCGACCTCTTCGGGAGAGGTCAGACGCGTCGGAAAAAAAATAGAGGCTTA‑AAGCTGTGGGCC‑TCCCCGGGCGGCGTACC‑CCCGAGTTGACCCGCCGCAACCTGGGCGtt < 2:522939/141‑1 (MQ=255) cgGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTgcgc > 1:4219/1‑151 (MQ=255) gggACCCATCCTGATTCTCTTGCAGCAGAATTTTGCCTAACCCTCCAGTAGTGGTCAGACGCCTCGGACAAATAATAGGGGCGTT‑AAGTTGTT‑GCCTTTCCCCGGCGGCGCACC‑ACCGAGCTGACCCGCCGCAACCTGGGCg < 2:208600/142‑1 (MQ=255) ggaacccATCTTGTTTCCTTTTCAGCAGGTTTTTGCCGAACTTTTCAGTAGGGGTCAGCCGCGTCTGAGAAATAATAGGGGCGTT‑AAGTTGTTGGCC‑TTCCCGGGGGGCGTACC‑CCCGAGCTGACCCGCCGCAACCTGGGCGTTCTgcgc < 2:326365/145‑1 (MQ=255) accctccttgtttCACTTCCAGGAGTTTTTGGCCGACCTCTACAGGAGGGGACAGCCGCGTCAGAAAAAAAAGAGGGGGGTT‑AGGTTGTTGGCC‑TCCCCCGGGGGCGTACC‑CCCGACCGCACCCGCCGCAACCTGGGCGTTCTGCGCt < 1:309260/137‑1 (MQ=255) acccATCCTGATCCCTTTCCAGAAGTTTTTTGCCGACCTCTTAAGAGGAGGTCAGCCGCGTCTGAG‑AAAAAAAGGGGTGTTAAAGGTG‑TGGCCCTCCCCGGGCGGCGTCCC‑CCCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTg < 1:705567/149‑1 (MQ=255) aaCCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTg > 2:483260/1‑151 (MQ=255) aaCCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTg > 1:179090/1‑151 (MQ=255) cacccTGTTTCACTTTCAGCAGTTTTTTCCCGATCTCTTGAGTAGGGGCCAGGCGCGTCGGAAAAAAAAAAGGGGCTTA‑AAGCTGTTGGCC‑TCCCCGGGCGGCGTCCC‑CCCGAGCGGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATg < 1:483208/148‑1 (MQ=255) tGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGt > 1:169349/1‑151 (MQ=255) tttCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCAACGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATTACAt > 2:195787/1‑151 (MQ=255) tcaaggagttttttGCCGATCTTTTCAGTAGAGGCCAGCCGCTTCTGAGAAATAAAAGGGGCGTT‑AAGTTG‑TGGTCCTCCCCCGGCGGCTTCCC‑CCCGGGCGACCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACAt < 2:748546/140‑1 (MQ=255) tttttGCCAACCTTTAAAGTAGAGGTCAGCCGCTTCTGAGAAAAAATAGGGTTTTT‑AATGTGTT‑GCCCTTCCCCGGCGGCGTCCC‑CCCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCgg < 1:185313/147‑1 (MQ=255) aTTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGt > 2:392705/1‑151 (MQ=255) ttGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 1:528529/1‑149 (MQ=255) cGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTgg > 1:574935/1‑151 (MQ=255) ggagggtcaacccgcgTCTGATAAATAAGGGGGGGTTT‑AGGCTGTGGGCC‑TCCACGGGGGGCGCCCC‑CCCGACCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTg < 1:670212/134‑1 (MQ=255) tCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCAtt > 1:252579/1‑150 (MQ=255) cgTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTc > 2:156037/1‑150 (MQ=255) gCAATAATAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATcc > 1:424464/1‑147 (MQ=255) aataatAGAGGCGTT‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCCGGTTCATCCAGATAc > 2:279129/1‑151 (MQ=255) ggggCGTT‑AGGTTG‑TGGGCTTTCACCGGCGGGGCCCC‑ACCGGGCGGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATcg < 1:245448/148‑1 (MQ=255) t‑AAGCTGTTGGCC‑TTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtgt > 1:12481/1‑151 (MQ=255) | GTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTT‑AAGCTGTTGGCCTTTCACCGGCGGCGTACC‑ACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGT > NC_000913/483579‑483864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |