Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,586,594	(G)6→5	coding (168/3513 nt)	hsdR ←	endonuclease R Type I restriction enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,586,589	0	G	.	100.0%	311.2 / NA	64	coding (173/3513 nt)	hsdR	endonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (33/31);  total (33/31)

AGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGG  >  NC_000913/4586439‑4586735                                                                                                                                                       |                                                                                                                                                   aGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAg                                                                                                                                                    <  1:412137/151‑1 (MQ=255)    cacaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggg                                                                                                                                                 >  2:265611/1‑151 (MQ=255)    cacaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAggg                                                                                                                                                  <  1:223249/150‑1 (MQ=255)       aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggat                                                                                                                                              >  1:479104/1‑149 (MQ=255)         cTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggatgt                                                                                                                                            >  1:594904/1‑147 (MQ=255)         cTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggatgt                                                                                                                                            >  2:364848/1‑147 (MQ=255)             gCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAg                                                                                                                                       <  1:605612/151‑1 (MQ=255)             gCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAg                                                                                                                                       <  2:325669/151‑1 (MQ=255)              cATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGt                                                                                                                                      >  2:375194/1‑151 (MQ=255)                     gagaTCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGAc                                                                                                                               <  1:493316/151‑1 (MQ=255)                      agaTCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGAcc                                                                                                                              <  1:263726/151‑1 (MQ=255)                         tCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCa                                                                                                                             >  2:352550/1‑149 (MQ=255)                          cGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCa                                                                                                                             >  1:231492/1‑148 (MQ=255)                          cGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGa                                                                                                                          <  1:99898/151‑1 (MQ=255)                                   agtCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGATCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGc                                                                                                                  <  1:3166/148‑1 (MQ=255)                                         gCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCt                                                                                                           <  1:740228/151‑1 (MQ=255)                                          cACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCtt                                                                                                          >  1:639608/1‑151 (MQ=255)                                           aCCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTc                                                                                                         >  2:696652/1‑151 (MQ=255)                                               ccTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGcc                                                                                                      >  2:219937/1‑150 (MQ=255)                                               ccTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCa                                                                                                     <  2:396460/151‑1 (MQ=255)                                                 tGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCaa                                                                                                    >  1:395631/1‑150 (MQ=255)                                                  ggTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCaaa                                                                                                   <  1:403061/150‑1 (MQ=255)                                                  ggTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAAc                                                                                                  >  2:636721/1‑151 (MQ=255)                                                      aCCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATAc                                                                                              >  1:115219/1‑151 (MQ=255)                                                            gcggcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAttt                                                                                        >  2:27722/1‑151 (MQ=255)                                                              ggcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAtttt                                                                                       >  2:242553/1‑150 (MQ=255)                                                                 gTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATc                                                                                   <  1:711301/151‑1 (MQ=255)                                                                  tAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATc                                                                                   >  2:119409/1‑150 (MQ=255)                                                                   aaTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATc                                                                                   >  2:220713/1‑149 (MQ=255)                                                                   aaTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATc                                                                                   >  2:267012/1‑149 (MQ=255)                                                                     tttGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGcg                                                                               >  2:635376/1‑151 (MQ=255)                                                                     tttGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGcg                                                                               >  2:198231/1‑151 (MQ=255)                                                                      ttGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGcgt                                                                              >  2:643345/1‑151 (MQ=255)                                                                      ttGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGcg                                                                               <  2:447033/150‑1 (MQ=255)                                                                          gAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCgtg                                                                          <  2:284915/151‑1 (MQ=255)                                                                             aTACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTg                                                                       <  1:723626/151‑1 (MQ=255)                                                                             aTACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTg                                                                       >  1:670696/1‑151 (MQ=255)                                                                              tACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTgg                                                                      >  1:472617/1‑151 (MQ=255)                                                                               aCAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTggg                                                                     >  1:771563/1‑151 (MQ=255)                                                                                cAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTggg                                                                     >  1:297090/1‑150 (MQ=255)                                                                                  gagagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGatc                                                                  <  1:751986/151‑1 (MQ=255)                                                                                    gagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGatcat                                                                >  2:753965/1‑151 (MQ=255)                                                                                     agGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAtttt                                                                                       <  2:438499/127‑1 (MQ=255)                                                                                     agGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAtttt                                                                                       >  1:438448/1‑127 (MQ=255)                                                                                            tgtCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGt                                                          >  2:402958/1‑149 (MQ=255)                                                                                               catcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTa                                                     <  1:593391/151‑1 (MQ=255)                                                                                                 tcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTAtt                                                   >  1:225398/1‑151 (MQ=255)                                                                                                  caacaaAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTAtt                                                   <  1:264539/150‑1 (MQ=255)                                                                                                   aacaaAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTAtttt                                                 <  1:743399/151‑1 (MQ=255)                                                                                                         ggCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTccgccg                                           <  2:395680/151‑1 (MQ=255)                                                                                                         ggCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTccgccg                                           <  1:654807/151‑1 (MQ=255)                                                                                                          gCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCcgccgc                                          <  1:747228/151‑1 (MQ=255)                                                                                                           cGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCcgccgc                                          >  1:19195/1‑150 (MQ=255)                                                                                                             aTTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGcaca                                       <  2:171247/151‑1 (MQ=255)                                                                                                                  gCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGa                                  <  1:375147/151‑1 (MQ=255)                                                                                                                      aGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGc                              >  2:672534/1‑151 (MQ=255)                                                                                                                       gTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCa                             <  2:595037/151‑1 (MQ=255)                                                                                                                         tCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCAt                            >  2:585473/1‑150 (MQ=255)                                                                                                                          cACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATa                           <  1:198198/150‑1 (MQ=255)                                                                                                                              cAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGa                      <  2:231525/151‑1 (MQ=255)                                                                                                                                  agaTCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTc                  >  1:387735/1‑151 (MQ=255)                                                                                                                                  agaTCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTc                  <  2:139050/151‑1 (MQ=255)                                                                                                                                     tCGTGTTGATTTTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGCTGGCATAAGTGAAGTCGtt               >  1:510988/1‑151 (MQ=255)                                                                                                                                      cGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCGGCACAGGCTATGGCATAAGTGAAGTCGtt               <  1:424391/150‑1 (MQ=255)                                                                                                                                        tgtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGAc            >  2:601263/1‑151 (MQ=255)                                                                                                                                         gtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACg           >  2:24741/1‑151 (MQ=255)                                                                                                                                             aTTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCt       <  2:540682/151‑1 (MQ=255)                                                                                                                                              ttCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCtt      >  2:44077/1‑151 (MQ=255)                                                                                                                                               tctcACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCtt      <  1:112537/150‑1 (MQ=255)                                                                                                                                                  cacaA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAgg  <  2:695200/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   AGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGG  >  NC_000913/4586439‑4586735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑