| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,967,611 | (C)5→6 | coding (891/1050 nt) | cheB ← | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,967,606 | 1 | . | C | 86.7% | 86.3 / 8.5 | 30 | coding (896/1050 nt) | cheB | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Reads supporting (aligned to +/- strand): ref base . (0/4); new base C (16/10); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑G‑CCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGG > NC_000913/1967458‑1967751 | aCGTATCGCCTGTCCGGCACTAATTTTTGCAAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑G‑c < 1:392888/150‑1 (MQ=255) gcGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑G‑ccccc > 1:402656/2‑149 (MQ=255) ccTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑G‑ccccccgcct < 2:204270/151‑6 (MQ=255) ccTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑G‑ccccccgcct < 1:59014/151‑6 (MQ=255) tGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTg > 2:624454/1‑150 (MQ=255) ccGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGc > 1:545749/1‑151 (MQ=255) tttGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACAtt > 2:759465/1‑151 (MQ=255) tGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑cc > 2:686111/1‑151 (MQ=255) tGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑c < 1:218492/150‑1 (MQ=255) gCCAACATTTGCTGGCTTACCTGTCTAAGATCGACCACTTCGCAGACACCACCCATATGGATGGCCGCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑ccc < 1:564974/151‑1 (MQ=255) tGCTGGCTTACCTCGCTACGATCGACCACTGCGCAGACACCACCCATATTGAGGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCACGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCcgccacgcc > 1:336172/1‑150 (MQ=255) gCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCcgt > 2:217785/1‑151 (MQ=255) aCCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGccc > 1:658597/1‑151 (MQ=255) ggCTAAGATCGCCCACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCCCGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATAc < 1:336779/151‑1 (MQ=255) cTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCg > 2:78463/1‑151 (MQ=255) cgccccctccGCAGACACCCCCCATATTGAGGGCCTCGCGCGGCATGCCAAACCCCACGCAACTTGCTTCGTTTTGCGAAGGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCAAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGa < 2:490152/142‑1 (MQ=255) gACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGAt > 2:687146/1‑151 (MQ=255) cccctccGCAGACCCCACCCATATGGATGGCCTCGCGCGGCATGCCGACCCCCCCGCAACTTGCTTCGTTTTGCGCAAGGGTCCAGC‑C‑CCCCCGCCTGCCGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGAt < 2:37259/143‑1 (MQ=255) ccACTTCGCAGACCCCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGAt < 1:381661/149‑1 (MQ=255) ccACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCTTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAgg < 2:286695/147‑1 (MQ=255) ccACTTCGAAGACCCCCCCCATATTGATGGCCTCGCGGGGCATGCCGAACACCACGCATTTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATc < 2:445690/150‑1 (MQ=255) cACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACAGGTCAGGATCAc > 2:615521/1‑151 (MQ=255) tCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCAcccc > 2:27793/1‑150 (MQ=255) aGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCg > 2:711750/1‑151 (MQ=255) accccccccATATTGAGGGCCTCGCGCGGCAGGCCGAACCCCACGCAATTTGCTTCTTTTTGCGCAAGGGTCCT‑T‑GCCCCCCGCCTGACGCATCCCCACCATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAAcc < 1:430778/142‑1 (MQ=255) ccccccATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCACCTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGCCGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTa < 1:370060/148‑1 (MQ=255) ccTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTgg > 2:62291/1‑151 (MQ=255) ggctcgCCGAACCCCCCGCAACTTCCTTCGTTTTGCGAAAGGGCCCA‑T‑GCCCCCCGCCTGACGCACCGCCAACATC‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACa < 1:304835/143‑1 (MQ=255) gccATGCGAAACCCCACGCAATTTGCTTGGTTTTGCCCAGGGGTCCA‑TCC‑CCCCCGCCGAACGCTCCCCAAACTTG‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGAc < 1:624397/145‑1 (MQ=255) ccccgcatttctcTTCGTTTGGCGCAGGGGTCCAGC‑C‑CCCCCGCGTGACCCTCCGCCACCATC‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACAt < 1:399888/139‑1 (MQ=255) acGCAACTTGCTTCGTTTTGCACAAGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCt > 2:422469/1‑151 (MQ=255) ccaatctaGGGCCCA‑T‑C‑CCCCCCCCCGGCCGATCCCCCACCTTCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGAc < 2:170929/143‑1 (MQ=255) aGGGTCCA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGAc > 1:170896/1‑143 (MQ=255) ccA‑T‑GCCCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCgg > 1:719751/1‑151 (MQ=255) | ACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCA‑T‑G‑CCCCCGCCTGACGCATCGCCAACATT‑CCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGG > NC_000913/1967458‑1967751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |