Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,049,805	(T)5→6	coding (863/1095 nt)	gcvT ←	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,049,800	1	.	T	100.0%	119.2 / NA	33	G290S (GGC→AGC)	gcvT	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/21);  total (12/21)

AATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGG  >  NC_000913/3049651‑3049932                                                                                                                                                       |                                                                                                                                     aaTCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑t                                                                                                                                     <  2:572905/151‑1 (MQ=255)           tCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcggt                                                                                                                            <  2:194982/150‑6 (MQ=255)            cGCCAATGCCTTCCAGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGtc                                                                                                                            <  1:269162/150‑1 (MQ=255)            cGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGtca                                                                                                                           <  2:292561/151‑1 (MQ=255)             gCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGtcat                                                                                                                          <  1:255058/151‑1 (MQ=255)                  tACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGGGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCAcc                                                                                                                      >  1:5829/1‑150 (MQ=255)                    ccTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGa                                                                                                                   <  1:634060/151‑1 (MQ=255)                         cGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGaccaac                                                                                                              >  1:689790/1‑151 (MQ=255)                                  cgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTc                                                                                                     <  1:61056/151‑1 (MQ=255)                                   gcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTc                                                                                                     >  1:468341/1‑150 (MQ=255)                                      aGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTa                                                                                                 <  2:769742/151‑1 (MQ=255)                                          cAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCAt                                                                                             <  2:217036/151‑1 (MQ=255)                                                   aaCCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGc                                                                                     <  2:179785/150‑1 (MQ=255)                                                     cccAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGc                                                                                  <  1:280295/151‑1 (MQ=255)                                                             cGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAg                                                                          <  1:567231/151‑1 (MQ=255)                                                              ggggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGCGTGCCCGGCGCATGGGCAAAGCGTACCGGGAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAgg                                                                         >  1:125186/1‑151 (MQ=255)                                                                    aaaGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCa                                                                   <  2:429808/151‑1 (MQ=255)                                                                     aaGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCAc                                                                  >  2:26072/1‑151 (MQ=255)                                                                          ccGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCg                                                             <  2:76059/151‑1 (MQ=255)                                                                                gTGATAATGCCTTCATTCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTGCCAGGGCTTCCCGACCGATat                                                         <  1:469781/149‑2 (MQ=255)                                                                                 tGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGt                                                      >  2:233696/1‑151 (MQ=255)                                                                                   aTAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTc                                                     >  2:26885/1‑150 (MQ=255)                                                                                                       gCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGc                                <  1:4741/151‑1 (MQ=255)                                                                                                         ccTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCg                               >  1:558899/1‑150 (MQ=255)                                                                                                            gcgcATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGAt                             <  1:378896/149‑1 (MQ=255)                                                                                                                  cGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGc                     >  1:322300/1‑151 (MQ=255)                                                                                                                    gTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGccc                   <  1:53503/151‑1 (MQ=255)                                                                                                                       aaGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATg                <  1:744733/151‑1 (MQ=255)                                                                                                                              ccGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggcgg         <  1:261800/151‑1 (MQ=255)                                                                                                                              ccGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggcgg         >  2:303841/1‑151 (MQ=255)                                                                                                                                ggCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCt       <  1:653303/151‑1 (MQ=255)                                                                                                                                ggCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCt       <  1:191064/151‑1 (MQ=255)                                                                                                                                  cAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCCGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTa      >  2:650945/1‑150 (MQ=255)                                                                                                                                      tCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAgg  >  2:457732/1‑150 (MQ=255)                                                                                                                                      tCACTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATTCTCACGCTGCACTTCCAGAGCTTCGCGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAgg  <  1:206160/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                     AATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGG  >  NC_000913/3049651‑3049932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑