Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,351,196 | (G)7→8 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,351,189 | 1 | . | G | 97.6% | 137.0 / ‑2.6 | 42 | intergenic (‑150/+218) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (22/19); total (22/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 |
ATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTG > NC_000913/1351047‑1351333 | aTCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑gggggggg < 2:515875/151‑2 (MQ=255) ccTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggc < 2:771455/150‑3 (MQ=255) ttATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑ggggggggcgct > 1:496132/1‑146 (MQ=255) tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCtt > 1:561882/1‑151 (MQ=255) ttgttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCtttt > 1:707733/1‑151 (MQ=255) ttgttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCtttt > 1:192308/1‑151 (MQ=255) gttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTc < 2:61621/150‑1 (MQ=255) tgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTcccc > 1:369768/1‑151 (MQ=255) gCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCa < 1:522027/150‑1 (MQ=255) ttgttgTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑ccc > 1:590235/1‑151 (MQ=255) ttgttgTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTACCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCA‑ccc > 1:669159/1‑151 (MQ=255) gAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCctct < 1:670575/149‑1 (MQ=255) gAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTcc < 2:455707/151‑1 (MQ=255) aaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCa < 2:192918/151‑1 (MQ=255) ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAggg < 2:440232/151‑1 (MQ=255) cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGc < 2:707807/151‑1 (MQ=255) cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGc < 2:382960/151‑1 (MQ=255) cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGc < 2:192341/151‑1 (MQ=255) cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCCAACCCTCTCCCCAGAgggg > 1:429281/1‑150 (MQ=255) aTTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCg > 2:118243/1‑150 (MQ=255) ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGC‑TTTCCCCTCA‑CCCTAAACCTCTCCCCCGAGGGGCGa > 2:582319/1‑149 (MQ=255) tcgtcgTTTTAGTAAACAGTACGAACAAATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGGCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGa < 1:752451/149‑1 (MQ=255) gtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAgggg > 2:142370/1‑151 (MQ=255) tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCC‑CCCCAACCCTCTCCCCCGCGGGGCGAGGGGACCg > 1:109870/1‑150 (MQ=255) tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 2:586056/150‑1 (MQ=255) aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCA‑CCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATtgt > 1:504818/1‑150 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTTTGCAAatat > 1:425176/1‑151 (MQ=255) cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt < 1:186863/151‑1 (MQ=255) aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt < 1:708631/151‑1 (MQ=255) cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTa < 2:655865/151‑1 (MQ=255) cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGATTTTCCCCTTA‑CCCCAACCCTCTCCCCCGAGGGGCGAGGGGACCGGATTGTGGAAATATTGt > 1:218675/1‑149 (MQ=255) aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCA‑CCCCAACCCCCCCCCCCAAGGGGCGGGGGGGCCGGATTTTGCAAAAATTTTTTcccc > 2:733500/1‑150 (MQ=255) tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCCCATCCCACCCCCCA‑AGGGGGGGGGGGGCCCACTTCTGCCAAAATTGGTAcccacacaa > 2:211326/1‑142 (MQ=255) tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGGATTTTGCAAATATTGTTACCCCAGcaa > 2:142053/1‑148 (MQ=255) tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaaca < 1:733426/150‑1 (MQ=255) ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg < 2:104278/151‑1 (MQ=255) aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc < 2:561954/150‑1 (MQ=255) cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCCCC‑CCCTAACCCCCTCCCCAGAGGGGGGAGGGGAACCGATTTTGCAAAAATTTTTAACCCCGCCACAAAAAAGCTc > 2:691151/1‑150 (MQ=255) ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAATATTTTTACCCCCACAACAAACCAGCTCAAACa > 1:657834/1‑149 (MQ=37) aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCAt < 2:525780/150‑1 (MQ=255) aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCAt < 1:24156/150‑1 (MQ=255) gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCC‑CCCCAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg > 1:179435/1‑151 (MQ=255) cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCC‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCCACAAAAAGGGTCCTACAGCCCCTAACCCTTTCATGGCGAt > 2:247310/1‑150 (MQ=38) cGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCCGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCCTGGCGATGGCTGGGACgg > 1:289268/1‑151 (MQ=255) gCGGCAGGGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTg < 2:212264/151‑1 (MQ=255) | ATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCA‑CCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTG > NC_000913/1351047‑1351333 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |