Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	941,713	0	T	G	47.2%	73.5 / 22.2	53	V252G (GTG→GGG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (3/25);  new base G (25/0);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 4.40e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCG  >  NC_000913/941569‑941861                                                                                                                                                 |                                                                                                                                                     actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACt                                                                                                                                                  <  1:325355/149‑1 (MQ=255)    cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTa                                                                                                                                             <  2:24517/151‑1 (MQ=255)       ccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCt                                                                                                                                          <  1:245028/151‑1 (MQ=255)              aaTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGAAGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGGAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGaacttctatttttaa                                                                                                                                      >  1:228511/1‑134 (MQ=255)                  gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCa                                                                                                                                <  1:644700/150‑1 (MQ=255)                  gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCAc                                                                                                                               <  2:241408/151‑1 (MQ=255)                  gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTATTTTCTTTAACAGGCAc                                                                                                                               >  1:430719/1‑151 (MQ=255)                   cGGAAGGTTTGAACTATACCTACGGCTGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCAc                                                                                                                               <  2:596142/150‑1 (MQ=255)                     gAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGcc                                                                                                                            <  2:146785/151‑1 (MQ=255)                                 tataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTaa                                                                                                                  >  2:607828/1‑149 (MQ=255)                                 tataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCCGAAAACTaa                                                                                                                  >  2:88028/1‑149 (MQ=255)                                 tataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTTAAAAAGCCAGCCAGAAATCtta                                                                                                                  >  2:483884/1‑147 (MQ=255)                                  ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATg                                                                                                                <  2:407176/150‑1 (MQ=255)                                    aCCTACGTCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGccc                                                                                                             <  2:285531/151‑1 (MQ=255)                                          ggcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGa                                                                                                       <  2:197968/151‑1 (MQ=255)                                           gcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGat                                                                                                      <  2:693331/151‑1 (MQ=255)                                             ggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGc                                                                                                           >  2:110357/1‑144 (MQ=255)                                              gcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcat                                                                                                   <  2:184109/151‑1 (MQ=255)                                                tGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAAGCACGCCCGAAATCTAATGCCCGCATGataa                                                                                                    >  1:221373/1‑146 (MQ=255)                                                 gggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGGACGCCAGAAAAATAATGCCCCCACGatca                                                                                                    >  1:63027/1‑147 (MQ=255)                                                    cAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAAAAGGCACGCCAGAAAACTAATGCCCGCCTGatca                                                                                                    >  2:760321/1‑144 (MQ=255)                                                     aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTACTATTTTGAACAAGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGa                                                                                             >  1:119178/1‑150 (MQ=255)                                                         ggCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATTTTTAACAGGCACGCCCGAAAACTAAAGGCCCCCTGGTCATCATCGAttc                                                                                          >  1:661552/1‑147 (MQ=255)                                                             aCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCta                                                                                    <  2:637904/151‑1 (MQ=255)                                                             aCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAGTTAATTTCTTGAACAGGCCCGCCAGAAATCTAATGCCCGCATGatcatcaactgtcc                                                                                          >  2:308608/1‑138 (MQ=255)                                                                    gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTTAAAAGGGACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGa                                                                              >  2:371348/1‑150 (MQ=255)                                                                           atCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCgggg                                                                      >  2:600123/1‑149 (MQ=255)                                                                                   cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGgcg                                                               >  2:102097/1‑150 (MQ=255)                                                                                    aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTACTATCTTTAACAGGCACGCCCGAAAACTAAAGCCCGCACGATCATCCTCGATCCGCGCCATACCGCCCCCGGCGACgg                                                                  >  1:63609/1‑146 (MQ=255)                                                                                    aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTCGGGGGGCTTACTATCTTGAACAGGGCCGCCCCAAATCTAATGGCCGCCTGGTCATCATCGCTCCGCGCTATAACGACACCGGCGGCCGgcgc                                                              >  1:544760/1‑150 (MQ=255)                                                                                       aaGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGaa                                                           <  2:661610/150‑1 (MQ=255)                                                                                       aaGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAg                                                          <  1:165663/151‑1 (MQ=255)                                                                                         gCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGAt                                                        <  2:132187/151‑1 (MQ=255)                                                                                             gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCCCGCTATACCGACACCGGTGGCGGGGGCGGAGATGAg                                                     >  1:272865/1‑150 (MQ=255)                                                                                               aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTgg                                                  <  2:102554/151‑1 (MQ=255)                                                                                               aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTg                                                   <  2:539968/150‑1 (MQ=255)                                                                                               aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTg                                                   <  1:672309/150‑1 (MQ=255)                                                                                                 tGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGTgg                                                  >  1:203247/1‑149 (MQ=255)                                                                                                          gTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCg                                       <  2:286794/151‑1 (MQ=255)                                                                                                            aataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAAGCACGCCCGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCCGGGCCGGGCGCGGAGATGAGTGGATCCCTATTCGt                                      >  1:513574/1‑150 (MQ=255)                                                                                                                aCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAAGCACGCCAGAAATTTTATGCCCGCCTGATCATCATTGCTCCGCGCTATAACGACACCGGTGGCGGGCGCGGAGATTAGGGGAACCCTaattttcccgg                                 >  1:533824/1‑141 (MQ=255)                                                                                                                  ccTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGAAACCGGTGCCGGGCGCGGAGATTAGTGGGTCCCTATTCTTCCgg                                  >  2:457007/1‑148 (MQ=255)                                                                                                                   cTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTTGACAGGGACGCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCCATACCGACACCGGGGCCGGGGGCGAAGATGAGTGGATCCCTATTTGTCCGGGa                                >  1:400167/1‑149 (MQ=255)                                                                                                                     ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAAc                              <  1:292418/149‑1 (MQ=255)                                                                                                                     ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAg                            <  2:221405/151‑1 (MQ=255)                                                                                                                       cGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcatc                                                                                               <  1:712487/82‑1 (MQ=255)                                                                                                                       cGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGatcat                                                                                                   >  2:712561/1‑78 (MQ=255)                                                                                                                           aCGCGAATGAGTGGCGGTGGGGGGACTTTATATTTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATTATCGATCCCCGCTATAACGACACCCGGGCCGGGCGCGAAGATGAGTGGATTCCTATTCGTCCCGGAACAGATGcc                       >  1:1451/1‑150 (MQ=255)                                                                                                                               gAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCCATCCGCGCTATACCGACACCCGGGCCCGGCGCGAAGATGAGTGGATCCCTATTCGTCCCGGGACAGATGcccccc                   >  2:388436/1‑146 (MQ=255)                                                                                                                                        gCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACgg         <  1:659646/151‑1 (MQ=255)                                                                                                                                              gggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATg                         <  2:413016/129‑1 (MQ=255)                                                                                                                                              gggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATg                         >  1:412965/1‑129 (MQ=255)                                                                                                                                               ggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCg  >  1:413363/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                     ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCG  >  NC_000913/941569‑941861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑