Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,483,268 | 0 | T | A | 50.9% | 45.2 / 86.4 | 57 | L24F (TTA→TTT) | emrK | multidrug resistance efflux pump membrane fusion protein |
Reads supporting (aligned to +/- strand): ref base T (17/11); new base A (0/29); total (17/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.54e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-12 |
TTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGT > NC_000913/2483119‑2483415 | ttCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat > 1:597056/1‑150 (MQ=255) tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata < 2:136152/150‑1 (MQ=255) gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATaaaa < 2:574231/151‑1 (MQ=255) ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAcc < 2:585897/150‑1 (MQ=255) tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg < 1:230397/150‑1 (MQ=255) acagaaacacTACCGGAGATTTGTGAAAAAATTGATCCTGCATCCCCCGTAACATGGGCGTCATCTTAACAAATCATTTCTTAAAATC‑CCATAGCCAAAAAGAAAAAGGCACCAAAAACCACAATAAAAAAAACTACCGCCAATAAAGaa < 1:282172/144‑1 (MQ=255) cacTCCCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATT‑CCATTGACCAATAGGCATAGGCCCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAttt < 2:699806/151‑1 (MQ=255) ccggagtCTTGTGCAAAAATTGGTTCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTAAAATT‑CCATTGCCAAATAGAAATAGACACCTAAAAACGAAAAAAAAAAAACTCCCGCCAATAAAGAAAAGTAtttt < 1:97870/144‑1 (MQ=255) ccggagaTTTGTGCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATC‑CCATTGCCAAATAGGCATAGGCACCTGAAAACGCAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTttct < 2:120535/146‑1 (MQ=255) ccTGAGATTTGTGCAGAAATTGGATCTCCATTCCCCGTGACATAGGCTTCATCTGTACTAATCATGTTTTCTAATT‑CCATTGACCAATAGGCATAGGCCCCTAAAAACCCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTttct < 2:411401/149‑1 (MQ=255) ccTGAGATTTGTGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCTTCATCTGTACTAATCATTTCTTCAAATT‑CCATTGACCAATAGGCATAGGCACCTGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTttc < 2:589284/148‑1 (MQ=255) ccTGAGACTTGTGAAGAAATTGGATCTCCATCCCCCGTGACATAGGCGTCATTTGTACTAATCATGTCTTCTAATC‑CCATTGACAAATAGGAATAGGCCCCTGAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt < 2:732550/150‑1 (MQ=255) agttttGTGCAAAAATTGTATCTCCTTTCCCCGTGACATAGGCGTCATCTGTACTAATAATGTTTTATAATT‑CCATTGCCAAAAAGAAATAGCCACCTGAAACCAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTtcttct < 1:495991/144‑1 (MQ=255) gaCTTGTGCAGAAATTGGATCGGCATTCCCCGTAACATAGGCGTCATCTGTACTAATCATGTTTTCTAATC‑CCATTGACCAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt < 2:647140/150‑1 (MQ=255) gtgCAAAAATTGGATCTGCATTCCCCGTGACATGGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCCCCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt < 2:390668/150‑1 (MQ=255) tgaaGAAATTGGATCTTCATCCCCCGTAACATAGGCGTCATTTGTACTAATAATGTCTTCTAATT‑CCATTGCACAATAGGCATAGGCACTTGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg < 2:218311/147‑1 (MQ=255) aGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATT‑CCATTGACAAAAAGAAATAGACACCTTAAAACAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt < 1:166533/149‑1 (MQ=255) gAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt > 1:704533/1‑151 (MQ=255) ttGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATC‑CCATTGACCAATAGGCAAAGGCCCCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt < 2:205887/150‑1 (MQ=255) ggATCTGCATCCCCGGGGCAAGAGGCATCATCTGTATAAAAAATGT‑TTATAATTGCAATTCACCAAGAGGAATAGGCCCGTAAAACGGAAATAAAAAAACTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt < 1:36203/150‑1 (MQ=255) tGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt > 1:292002/1‑150 (MQ=255) ccATTCCCCGTAACATAGGAGTCATCTGTATTAATCATTTCTTAAAATT‑CCATTGACCAATAGAAATAGGCACTTAAAAACGCAAAAAATAAAACTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt < 1:245259/147‑1 (MQ=255) tCCCCGTGACATAGGCTTCATCTGTCCTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACTTGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt < 2:263102/144‑1 (MQ=255) ccccGTGACAAAGGCTTCTTCTTTCCTAATAATTTCTTCTATTC‑CCATTCACAAATAGGCATAGGCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt < 2:184872/143‑1 (MQ=255) cGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCCCCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc < 2:226415/151‑1 (MQ=255) gTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc < 2:270810/150‑1 (MQ=255) aTAGGCGTCATCTGTACAAATCATGTCTTCAAATC‑CCATTGACAAATGGGCATAGGCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat < 2:455390/150‑1 (MQ=255) tAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt > 2:504624/1‑150 (MQ=255) tAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt > 2:549112/1‑150 (MQ=255) cATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctctc > 1:281662/1‑150 (MQ=255) tGTACAAATAATGTTTTTTATTC‑CCATCGACAAATAGACATAGGCCCATAAAAACGAAATAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctct < 1:151381/145‑1 (MQ=255) tACAAATCATGTCTTCTAATC‑CCATTGACCAATAGGCATAGCCCCCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAt < 2:630222/146‑1 (MQ=255) aCTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtctc > 2:547710/1‑150 (MQ=255) aaTCTTGTCTTCTAATT‑CAATTGACCAATAGGAATAGGCACTTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt < 2:222208/149‑1 (MQ=255) aaTCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt > 1:682482/1‑149 (MQ=255) aTCATTTCTTCTAATT‑CCATTGCCCAATAGGCATAGGCCCCTGAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGa < 2:653170/151‑1 (MQ=255) tcttctAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAt > 2:264143/1‑151 (MQ=255) cttctAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt > 2:653652/1‑151 (MQ=255) ttctAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAttt > 1:529299/1‑151 (MQ=255) aaTT‑CCATTGACAAATAGGCATAGGCACCTAAAAACAAAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAttt < 1:275624/147‑1 (MQ=255) aaTT‑CCATGAACAAATAGGCATAGGCCCCTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg < 1:502838/150‑1 (MQ=255) tt‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaat > 1:268582/1‑151 (MQ=255) t‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt > 1:106821/1‑151 (MQ=255) ccAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatgaa > 2:445210/1‑150 (MQ=255) aaTAGGCATAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag < 2:569687/151‑1 (MQ=255) ataggtatgggcACCTGAAAACAAAAAAAATAAAGCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat < 2:119043/144‑1 (MQ=255) taggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAag > 2:336353/1‑149 (MQ=255) atagccaCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcat < 2:419535/149‑1 (MQ=255) taggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattc > 1:35643/1‑150 (MQ=255) ggcaCCTGAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt < 2:79557/151‑1 (MQ=255) caCCTAAAAACCCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt < 1:707427/149‑1 (MQ=255) aaatCGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTATGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCa < 1:583637/148‑1 (MQ=255) aaaaCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCatat > 1:343698/1‑151 (MQ=255) aaaaCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt < 1:265466/146‑1 (MQ=255) aaaaCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCata < 2:273345/150‑1 (MQ=255) aaaaCGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat < 2:299333/149‑1 (MQ=255) aaaaCGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat < 1:77403/149‑1 (MQ=255) aaaCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATc > 2:726386/1‑151 (MQ=255) aCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCaa < 1:428037/151‑1 (MQ=255) aaaaaaaaaaaATACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCa < 2:722492/143‑1 (MQ=255) aaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt > 2:599974/1‑151 (MQ=255) | TTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATT‑CCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGT > NC_000913/2483119‑2483415 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |