Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,504	0	T	A	42.6%	73.2 / 24.4	54	K290* (AAA→TAA)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (21/10);  new base A (0/23);  total (21/33)
Fisher's exact test for biased strand distribution p-value = 9.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACC  >  NC_000913/4286356‑4286652                                                                                                                                                     |                                                                                                                                                     ggCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTaa                                                                                                                                                    <  2:142482/151‑1 (MQ=255)      cGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGt                                                                                                                                                <  1:26037/150‑1 (MQ=255)       ggCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTc                                                                                                                                               >  1:402772/1‑150 (MQ=255)       ggCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCa                                                                                                                                              <  1:18885/151‑1 (MQ=255)          cAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAgcg                                                                                                                                           <  1:646927/151‑1 (MQ=255)           aGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAgcgc                                                                                                                                          <  1:760421/151‑1 (MQ=255)                 gTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCAACAGTAGATATCAACCGGATGCTAATCTAAGAACTATTTAAAGGTCAGCGCCGCGt                                                                                                                                     >  1:650866/1‑150 (MQ=255)                        ttCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACAcc                                                                                                                                >  1:645889/1‑148 (MQ=255)                          cAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAg                                                                                                                              >  1:102601/1‑148 (MQ=255)                              cATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAAccg                                                                                                                       <  1:2573/151‑1 (MQ=255)                                    cGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCATATCGGCGGTGCCCCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGGCAGCGCCGCGGACACCAGATAACCGCCggg                                                                                                                  >  1:530431/1‑149 (MQ=255)                                          cGCCCCGCAGGCCGGCGGGCCGTACAGAAAGAACCTGTGCCCGGTCACCCACCCCACACCGGCGGTGCACCAGTAGATATCCCCCGGATGATAATAAAAGACATATTTAAAGGTCAGCGCCGCGTCCCCAAGATAACCGCCGGTAGTATg                                                                                                            <  1:684182/150‑1 (MQ=255)                                                   ggCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCCCCCAGCCCACATCGGCGGTGCCCCAGTAGATATCACCCGGATGATAATCAAAGACATATTAAAAGGTCAGCGCCGCGTCCACCAGATAACCGCCGGTAGTATGCAGCACACCt                                                                                                  <  2:386524/151‑1 (MQ=255)                                                     ccAGCGGGCCGTACAGCAAGAACCTGTGTCCGGTCACCCACCCCACATCGGGGGTGCACCAGTAGTTACCCCCCGGATAATAACCAAAGCAATATTAAAAGGCCCCCGCCGCGTACACCAGAAAACCGCCGGTAGTATGCAGCACACCtt                                                                                                 <  1:136730/150‑1 (MQ=255)                                                      cAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAAACGCCGGTAGTATGCAGCACACCtttt                                                                                               >  1:47509/1‑151 (MQ=255)                                                       aGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTg                                                                                              >  1:550790/1‑151 (MQ=255)                                                         cGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGc                                                                                            >  1:419248/1‑151 (MQ=255)                                                          tcccgatcaaaaaaGTAACTGTGCCCGGCCACCCACCCCACACCGGGGGTGCCCAAGTAAATATCCCCCGGATAATAACAAAAGACATATTAAAAGGTCAGCGCCGCGTACCCAAAAAAACCGCCGGTAGTATGCAGCACACCTTTTg                                                                                              <  1:393306/136‑1 (MQ=255)                                                          gggCCGTACAGCAAGTAACTGGGTCCGTTCCCCCAGCCCACATCGGCGTTGCCCCAGTAGATATCCCCCGGATGATAATCAAAGACATATTTAAAGTTCAGCGCCGCGTCCACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTgg                                                                                             <  2:567664/149‑1 (MQ=255)                                                                   aGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTg                                                                                              >  2:393355/1‑139 (MQ=255)                                                                   aGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGt                                                                                    >  2:666224/1‑149 (MQ=255)                                                                      aaatAACTGTGCCCGGTCCCCCAGCCCACAGCGGGGGGGCACCAGTAGATACCCCCGGGATGATAACAAAAGAATTTTTAAAAGGCCAGCGCGGCGTCCCCGAGAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAAc                                                                               <  1:511712/148‑1 (MQ=255)                                                                      aaGTAACTGTGTCGGGTCCCCCAGCCCCCTGGGGGGGGGCACAAGAGAATACCCCCCGGATAAAAACCAAAGCCATATTAAAGGGTCAGCGCCGCGTACCCAAGAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCgg                                                                                     <  1:534737/145‑1 (MQ=255)                                                                               tgtCCGGTCCCCCAGCCCACACCGGGGGTGCACAAGTAGATACCCCCCGGATAAAAATCAAAGAAATATTAAAGGGTCAGCGCCGCTTACCCAAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGgtg                                                                       <  2:313676/150‑1 (MQ=255)                                                                               tgtCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGgtg                                                                       >  1:167315/1‑150 (MQ=255)                                                                                gtCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGgtgt                                                                      >  2:515464/1‑150 (MQ=255)                                                                                gtCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGgtg                                                                       >  1:474995/1‑149 (MQ=255)                                                                                 tCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAAACGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAgggg                                                                       >  2:621993/1‑146 (MQ=255)                                                                                  ccGGTCCCCCAGCCCACAGCGGCGGTGCCCCAGTAAATACCCCCCGGATGATAATAAAAGACATATTAAAAGGTCAGCGCCGCTTACACCATATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTa                                                                     <  1:493163/149‑1 (MQ=255)                                                                                    ggccACCCAGCCCAAACGGGGGGTGCACCAGAAGATATCCCCCGGATAATAATAAAAGAAATTTTAAAAGGTCGGCGCGGCGTACACCAAAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTaga                                                                   <  1:672831/146‑1 (MQ=255)                                                                                        ccccaccccacACCGGCGGTGCCCCAGTAAATATCCCCCGGATAAAAATAAAAGACATATTAAAAGGTCAGCGCCGCGTACCCCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATaa                                                             <  2:689639/150‑1 (MQ=255)                                                                                          ccACCCCACACCGGGGGTAAACCATTAAATATCACCGGGAGGAAAATCAAAGAAATATTAAAGGTTCGCCCCCGCGCCCGACACATAGCCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAAc                                                           <  2:62083/151‑1 (MQ=255)                                                                                              cccACCGCGGGGGGGCACCGGTAGATATCCCCCGGAGAAAAACAAAAAAAATATTAAAAGGTGAGCGCCGCTTACACAAGATAGCCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACa                                                          <  1:683711/148‑1 (MQ=255)                                                                                              cccACATCGGCGGTGCACCAGTAGATACCCCCCGGATGATAATAAAAGAAATATTAAAAGGTCAGCGCCGCGTACACAAAAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGc                                                        <  1:279866/150‑1 (MQ=255)                                                                                              cccACACCGGCGGTGCCCCAGTAGATATCCCCCGGATAATAAACAAAGAAATTTTTAAGGGCCAGCGCCGCGTACCCCAAAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCg                                                       <  1:730628/151‑1 (MQ=255)                                                                                                caaaTCGCCGGTGCCCAATTAGATATCCCCGGGATGATAATAAAAGACATATTAAAGGGTACGCGCCGCGTACACAAGAAAGCCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCgg                                                      <  1:470933/147‑1 (MQ=255)                                                                                                 acacCGGCGGGGCACCAGTAGATACCCCCGGGATGATAATCAAAGAAATATTAAAGAGCCAGCGCCGCGTACACAAAAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGa                                                     <  1:363420/150‑1 (MQ=255)                                                                                                   ttCGGCGTGGCACCAGAATATACCCCCTGGAAGATAATAAAAGACATATTAAAAGTTCCGCGCGGTATCCACAAAATAGCCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCgg                                                      <  2:534935/146‑1 (MQ=255)                                                                                                   agcggcggTGCACCAGTAGATATCCCCGGGATAATAACCAAAAACATATTAAAAGGTCGGCGCCGCGTACCCCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCt                                                  <  2:475407/149‑1 (MQ=255)                                                                                                      ggcggTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCg                                               >  1:405527/1‑151 (MQ=255)                                                                                                      ggcggTGCACCAGTAGATACCCCCCGGATAAAAAACAAATACATATTAAAAGGCCAGCGCCGCGTACCCAAGAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCg                                               <  1:684072/151‑1 (MQ=255)                                                                                                      gcgggTACACCAGTAGAGATCCCCGGGATAAAAAGCAAAGAATTATTAAAAGGCCCGCGCCGGGACCCCAAAATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCg                                               <  2:91952/148‑1 (MQ=255)                                                                                                               ccAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCAtc                                      >  1:288322/1‑151 (MQ=255)                                                                                                                   tAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCtt                                  >  2:69519/1‑151 (MQ=255)                                                                                                                      ataccccccGGATAATAATAAAAGACATATTAAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTcc                                <  2:229689/144‑1 (MQ=255)                                                                                                                        accccccGGATGAAAATAAAAGAAATATTAAAAGGCCGGCGCCGCGTACACCAAAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGcc                             <  2:22238/147‑1 (MQ=255)                                                                                                                          ccccgggATGATAATCAAAGACATATTTAAAGGCCGGCGCCGCGTACACAAAATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCt                            <  2:347003/145‑1 (MQ=255)                                                                                                                            ccggtatgatAATAAAAGAATTATTAAAAGGTCAGCGCCGCGTCCACCAGAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTgg                          <  2:598173/145‑1 (MQ=255)                                                                                                                            cccGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTgg                          >  1:63131/1‑150 (MQ=255)                                                                                                                                    aaaacaaaaGAAATATTAAAAGGCCAGCGCCGCGTACACCAGAAAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTg                     <  2:316556/141‑1 (MQ=255)                                                                                                                                        tCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGAtcgctcg             >  2:82026/1‑151 (MQ=255)                                                                                                                                              aCATATTAAAGGGGCAGCGCCGCGTACCCCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTg         <  1:734893/149‑1 (MQ=255)                                                                                                                                                 tatTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGCAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGCTGCAGAGAATCAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGc        >  1:3786/1‑147 (MQ=255)                                                                                                                                                  atTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAAc   >  1:85292/1‑151 (MQ=255)                                                                                                                                                     tAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAAcc  >  2:96334/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACC  >  NC_000913/4286356‑4286652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑