Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,346,6750TG43.9% 57.5 / 12.6 41T218P (ACC→CCC) fumBanaerobic class I fumarate hydratase (fumarase B)
Reads supporting (aligned to +/- strand):  ref base T (5/18);  new base G (18/0);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.69e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGT  >  NC_000913/4346527‑4346821
                                                                                                                                                    |                                                                                                                                                  
aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTAc                                                                                                                                                  <  2:462620/151‑1 (MQ=255)
   gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCGGGCGTACCGCGGGTgcgc                                                                                                                                                >  1:717709/1‑150 (MQ=255)
    ccTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCAt                                                                                                                                              <  1:372678/151‑1 (MQ=255)
                 gttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGAACCCAGGGGACGCACTTTCTcgtcga                                                                                                                                   >  1:486162/1‑149 (MQ=255)
                    cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGCAACGCGGGGACGCATTTTCTTGACgggg                                                                                                                                 >  1:683566/1‑148 (MQ=255)
                          cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGttttt                                                                                                                         >  2:355499/1‑150 (MQ=255)
                            tCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCCTTTTCTCGACGAGGGAGttttt                                                                                                                         >  2:659611/1‑148 (MQ=255)
                             cGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGTACCGAGGGGACGCATTTTCTCGACGAGGGAGGTTTTTAg                                                                                                                      >  2:117326/1‑150 (MQ=255)
                              ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGGGGGGACGCATTTTTTCGACGAGGGAGGTTTTTAGtt                                                                                                                    >  2:71908/1‑151 (MQ=255)
                                        cGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGt                                                                                                          <  2:341995/151‑1 (MQ=255)
                                              aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTcagcag                                                                                                    <  1:387061/151‑1 (MQ=255)
                                                 gAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGc                                                                                                 <  1:180247/151‑1 (MQ=255)
                                                  aGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCt                                                                                                <  1:127667/151‑1 (MQ=255)
                                                   gcgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCtt                                                                                               <  1:322781/151‑1 (MQ=255)
                                                     gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTg                                                                                             <  1:682464/151‑1 (MQ=255)
                                                         gCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGCGGCTGCAGGACCGCGGGGACGCATTTTTTCGCCGAGGGAGGTTTTTTGTTTTCCGGGGGGCAGCAGGGGttttggtt                                                                                         >  1:511827/1‑146 (MQ=255)
                                                         acTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGGTGGAGGAACGAGGGGACGCATTTTTTCCACGAGGAAGATTTTTAATTTGTCGGGGGTAAACCGGGGTTTTGttt                                                                                         >  1:365221/2‑151 (MQ=255)
                                                             aCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTg                                                                                     <  2:715400/151‑1 (MQ=255)
                                                                    ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGGGTCAGCAGGGCTTTGGgtt                                                                                         >  2:393925/1‑140 (MQ=255)
                                                                          cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGAGGGGACGCATTTTTTCGGCGAGGGAGTTTTTTAGTTTTCCGGGAGTCAGCAGGGGTTTGGGTTCCTGGTTGAGAAAc                                                                           >  2:130657/1‑148 (MQ=255)
                                                                            ggttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtttt                                                                      <  1:564687/151‑1 (MQ=255)
                                                                                ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTttgtt                                                                   <  1:688859/150‑1 (MQ=255)
                                                                                         aGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCGAGGGGACGCATTTTTTCGACGAGGAAGGTTTTTAGTTTGTCGGGGGGCAGCAGGGCTTTGGTTTTCTGGTTGAGATACGTTTTTTTGGCAGAgcc                                                          >  2:650300/1‑150 (MQ=255)
                                                                                          gACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgc                                                        <  2:539231/151‑1 (MQ=255)
                                                                                               aCCGCCAATCACAAACGCGATATGGTACGGCGGGGCGGGTGCAGGACCCAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGGGTCAGGAGGGCTTTGGGTTTCTTGTAGAGATACGGTTTTTTGGCAGAgccgccgcc                                                    >  1:320366/1‑150 (MQ=255)
                                                                                               aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGGAGGTTTTCAGTTTGCCGGGGGGCAGCAGGGGTTTTGGTTCCTGGTAGAGATAAGTTTTGTTGGCAGAgccgccgcc                                                    >  1:127209/1‑150 (MQ=255)
                                                                                                  gCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTc                                                <  1:768556/151‑1 (MQ=255)
                                                                                                    cAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcg                                               <  2:644581/150‑1 (MQ=255)
                                                                                                     aaTCACAAACGCGATATGGTACGGCGGGGAGGATCCCGGGTCGACGGGACACAATTTTTCGGCGAGGGAGGTTTTCTGATTGCCGGGGGTCAGCGGGGGTTTGGGTTTCTTGTTGGGGTACGGTTTCTTTGGCGGgccgccgcc                                                    >  1:395065/1‑144 (MQ=255)
                                                                                                     aaTCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTCGTTTTCCGGGGGGCAGCAGGGGTTTGGGTTTCTGGGAGAGGTACGGTTTGTTTGGAGAGGCGCCGCCCTTtgcc                                             >  1:250273/1‑150 (MQ=255)
                                                                                                      aTCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaa                                            <  1:375356/151‑1 (MQ=255)
                                                                                                      aTCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGGTTTTTAGTTTGCCGGGGGTCAGCCGGGCTTTGGTTTTCTGGGAGAGATACGTTTTGTTTGCCGAGCCGCCGCCTTTcgcc                                             >  2:3387/1‑149 (MQ=255)
                                                                                                             aCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAg                                     <  2:699941/151‑1 (MQ=255)
                                                                                                                cgATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGAAGCATTTTTTCGACGAGGGAGGTTTTTTGGTTTCCGGGGGGCAGGAGGGGTTTGGGTTTCTGGGAGAGATAAGTTTTGTTGGCCGAGCCGCCCCCCTTTGCAACGCAAAgg                                    >  1:624481/1‑149 (MQ=255)
                                                                                                                   tatGGTACGGCGGGCAGGCTGCAGTACCGAGGGGAAGCATTTTTTTGACGAGGGAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGGTTTTGGTTTCTGGGAGAGATACGTTTTTTTTGGAGAGCCGCCGCCCTTTGCAACGCCAAAGAAatt                               >  1:435237/1‑151 (MQ=255)
                                                                                                                       gTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTAc                           <  2:363676/151‑1 (MQ=255)
                                                                                                                              gggCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc                    >  2:722917/1‑151 (MQ=255)
                                                                                                                                    gCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTa              <  2:381354/151‑1 (MQ=255)
                                                                                                                                            aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc      >  1:75032/1‑151 (MQ=255)
                                                                                                                                              cGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc      >  1:16988/1‑149 (MQ=255)
                                                                                                                                                aGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGt  >  1:417350/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGT  >  NC_000913/4346527‑4346821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑