Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	203,087	T→C	G176G (GGT→GGC)	lpxA →	UDP‑N‑acetylglucosamine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	203,087	0	T	C	100.0%	143.4 / NA	46	G176G (GGT→GGC)	lpxA	UDP‑N‑acetylglucosamine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

GTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCG  >  NC_000913/202944‑203230                                                                                                                                                |                                                                                                                                                gtaggtaACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGgcgc                                                                                                                                          <  1:461715/151‑1 (MQ=255)       aaCCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGGTGGCGACCTCGCGATCATCGGCGGCCAGACCCCAGTCCCACAGTTCTTCATCATTGGTGCGCCCGGGTTGGTTGGCGGCTGCCCCGGCGTGGCGCAGGa                                                                                                                                      >  1:200039/1‑149 (MQ=255)           gCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTTGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGGGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGGCGTccc                                                                                                                                >  2:682387/1‑151 (MQ=255)                aTTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTTGTGCGCACGGGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCtt                                                                                                                           >  2:739798/1‑151 (MQ=255)                     cGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATg                                                                                                                        <  1:360088/149‑1 (MQ=255)                     cGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGAGCAACGGCGGCATGGCCGCAGGCCATCAGGTCTGCATCATTTGTGCGCACGTGGTGGGTGGCGGCTGCTCCGGCGGGGCGGAGGACGTCCCCCCCTTTGt                                                                                                                       >  1:345029/1‑150 (MQ=255)                        caacaaCGCAACGCTGGCGGGTCACGGATCGGTTGCCGACATCCCGCACATCGGCGGCATTACCGCACGCCATCAGGTCTGCCTCATTGGGGCGCACGTGATGGGTGGCGGCTGCTCCCGCCGGGGGCCGGACGTGCCTCCTTATGTCAtt                                                                                                                   >  1:257998/1‑151 (MQ=255)                          acaacGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTgc                                                                                                                 <  2:628658/151‑1 (MQ=255)                             acgcaacgcTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCa                                                                                                              <  1:589523/151‑1 (MQ=255)                               gcaacgcTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAgg                                                                                                            <  1:698498/151‑1 (MQ=255)                                  acgcTGGCGGGTCACGTATCGGTTGACGACTTCGCGGTCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTTGTGCGCACGTGGTGGTTGGGGGGTGCTCCCGCGGGGGGCAGGGCGTCCCTCCTTATGTCATTTCGCAggg                                                                                                           >  1:680144/1‑149 (MQ=255)                                   cgcTGGCGGGTCCCGGATCGGTTGACGACTTCGCGGTCCTCGGCGGCATGACCGCAGTCCCTCAGTTCTGCATCATTTGTGCGCACGTGGTGGGTGGCGGCTGCTCCGGCGGGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGt                                                                                                          >  1:456482/1‑149 (MQ=255)                                   cgcTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCCTCATTGGTGCGCACGGGATGGTTGGCGGCTGCTCCGGCGTGGGGCAGGACGTCCCTCCTTATGTCCTTTCGCAggg                                                                                                           >  1:468049/1‑148 (MQ=255)                                       ggCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAAcc                                                                                                      <  2:549029/149‑1 (MQ=255)                                                     cGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCgg                                                                                      >  1:109231/1‑151 (MQ=255)                                                       gTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACTCCTTTCGgtg                                                                                    <  1:663900/151‑1 (MQ=255)                                                       gTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGgtg                                                                                    <  1:432116/151‑1 (MQ=255)                                                        ttGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGgtg                                                                                    >  1:320804/1‑150 (MQ=255)                                                            cgacTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAat                                                                               <  1:733401/151‑1 (MQ=255)                                                              acTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAatat                                                                             >  1:761689/1‑151 (MQ=255)                                                               cTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATc                                                                            >  1:175787/1‑151 (MQ=255)                                                                ttCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCg                                                                           >  1:538842/1‑151 (MQ=255)                                                                     gATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAggg                                                                      <  1:382384/151‑1 (MQ=255)                                                                     gATCATAGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAggg                                                                      >  1:71145/1‑151 (MQ=255)                                                                           cggcggCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAg                                                                <  2:67470/151‑1 (MQ=255)                                                                                  aTGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCcgcg                                                         <  1:530928/151‑1 (MQ=255)                                                                                  aTGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCGGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCcgcg                                                         >  1:251558/1‑151 (MQ=255)                                                                                   tGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCgg                                                        >  2:45352/1‑151 (MQ=255)                                                                                        gCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTc                                                    >  2:129966/1‑150 (MQ=255)                                                                                         cAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTc                                                    >  1:734387/1‑149 (MQ=255)                                                                                            tCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCg                                               >  2:742131/1‑151 (MQ=255)                                                                                                tCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTg                                             >  2:82041/1‑149 (MQ=255)                                                                                                    ttCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGc                                         >  1:742402/1‑149 (MQ=255)                                                                                                     tCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGAt                                      >  1:408777/1‑151 (MQ=255)                                                                                                        gCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGAtt                                     <  1:460474/149‑1 (MQ=255)                                                                                                               tGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTAt                             <  2:512903/150‑1 (MQ=255)                                                                                                                     gcACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAAt                      <  1:146860/151‑1 (MQ=255)                                                                                                                       aCGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGc                    >  1:248904/1‑151 (MQ=255)                                                                                                                           gATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGtat                <  2:604598/151‑1 (MQ=255)                                                                                                                            aTGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGtat                >  2:479094/1‑150 (MQ=255)                                                                                                                            aTGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGt                  <  1:103203/148‑1 (MQ=255)                                                                                                                                  ggcggcTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGa         <  2:663071/151‑1 (MQ=255)                                                                                                                                       cttctCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTAt    >  1:503088/4‑151 (MQ=255)                                                                                                                                         gctCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCg  <  1:513089/151‑1 (MQ=255)                                                                                                                                             cGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGta                 >  2:364872/1‑132 (MQ=255)                                                                                                                                             cGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGta                 <  1:364825/132‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                GTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCG  >  NC_000913/202944‑203230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑