Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,981	T→C	T383A (ACC→GCC)	ydhQ ←	autotransporter adhesin‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,981	0	T	C	100.0%	156.1 / NA	43	T383A (ACC→GCC)	ydhQ	autotransporter adhesin‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

CAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCATTCA  >  NC_000913/1744837‑1745127                                                                                                                                                 |                                                                                                                                                   cAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTc                                                                                                                                              <  2:243258/151‑1 (MQ=255)         aaCCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTc                                                                                                                                       >  2:697235/1‑150 (MQ=255)              tCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATtg                                                                                                                                 >  1:159825/1‑151 (MQ=255)               cACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATtga                                                                                                                                <  2:548199/151‑1 (MQ=255)                aCGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCACCGGCAACTTCCCCTTTCACATtga                                                                                                                                >  1:83738/1‑150 (MQ=255)                 cGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATtgatg                                                                                                                              >  1:378956/1‑151 (MQ=255)                  gggTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATtgatg                                                                                                                              >  2:185760/1‑150 (MQ=255)                     ttGCTCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCAAATTGATGAAGt                                                                                                                          >  1:466638/1‑151 (MQ=255)                       gcgcCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTc                                                                                                                         <  2:558390/150‑1 (MQ=255)                           cTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTg                                                                                                                    >  2:519698/1‑151 (MQ=255)                               aTTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGaaa                                                                                                                 <  2:640036/150‑1 (MQ=255)                                 ttttACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAAtt                                                                                                               <  2:177215/150‑1 (MQ=255)                                         aGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCa                                                                                                      <  2:752548/151‑1 (MQ=255)                                                   gTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGc                                                                                             >  2:481264/1‑150 (MQ=255)                                                       cgcCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGc                                                                                         >  2:12304/1‑150 (MQ=255)                                                       cgcCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCa                                                                                        <  1:65857/151‑1 (MQ=255)                                                        gcCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCaa                                                                                       <  2:516814/151‑1 (MQ=255)                                                              cccGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCt                                                                                 <  1:250967/151‑1 (MQ=255)                                                                      cGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTcc                                                                         <  1:65886/151‑1 (MQ=255)                                                                       gTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTcc                                                                         >  1:760671/1‑150 (MQ=255)                                                                               gTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAgg                                                                  <  1:147671/149‑1 (MQ=255)                                                                                 aaTTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGtt                                                              >  2:285823/1‑151 (MQ=255)                                                                                  aTTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGttt                                                             <  1:581855/151‑1 (MQ=255)                                                                                    ttCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTc                                                            >  1:378380/1‑150 (MQ=255)                                                                                             ttACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGtt                                                  >  1:251000/1‑151 (MQ=255)                                                                                                       tttGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGt                                        >  1:410937/1‑151 (MQ=255)                                                                                                        ttGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGt                                        >  2:122132/1‑150 (MQ=255)                                                                                                            cGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCg                                   >  1:276477/1‑151 (MQ=255)                                                                                                             gACTGGCATCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGc                                  >  1:702759/1‑151 (MQ=255)                                                                                                                    aTCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTg                           >  2:181785/1‑151 (MQ=255)                                                                                                                    aTCTGTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTg                           >  2:673396/1‑151 (MQ=255)                                                                                                                        gTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTg                           <  2:278347/147‑1 (MQ=255)                                                                                                                        gTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGAt                         <  1:292209/149‑1 (MQ=255)                                                                                                                        gTTATCTCAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCa                       <  2:726224/151‑1 (MQ=255)                                                                                                                               cAACGTGTATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGcc                    <  1:379329/147‑1 (MQ=255)                                                                                                                                     gtATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTcc          <  1:11894/151‑1 (MQ=255)                                                                                                                                     gtATATCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTcc          <  1:89556/151‑1 (MQ=255)                                                                                                                                       atatCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTcca         <  2:454724/150‑1 (MQ=255)                                                                                                                                          tCCCCGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCAt     >  2:381725/1‑151 (MQ=255)                                                                                                                                           ccccGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCAtt    >  2:254931/1‑151 (MQ=255)                                                                                                                                           ccccGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCAt     <  1:287151/150‑1 (MQ=255)                                                                                                                                             ccGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCATTCa  <  1:367929/151‑1 (MQ=255)                                                                                                                                             ccGGCAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCATTCa  <  1:463606/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTTTCACATTGATGAAGTCAGGTGAAATTTCTTTTGCACTGGTTTGCAGGCAAATCGCTCCCTCTCCGCGCAGGCGTTTCACTTTCAGTTGCACATGCGTTGCCGCATCAGTGATCAGCCACAGTTTTCCACCATTCA  >  NC_000913/1744837‑1745127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑