Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,240	T→C	E162G (GAA→GGA)	ydhW ←	FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,240	0	T	C	100.0%	185.7 / NA	51	E162G (GAA→GGA)	ydhW	FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

GCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCG  >  NC_000913/1751094‑1751384                                                                                                                                                   |                                                                                                                                                 gCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACCCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCaa                                                                                                                                               <  1:187338/150‑1 (MQ=255)         tCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCtt                                                                                                                                      >  2:728872/1‑151 (MQ=255)             aCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTg                                                                                                                                  >  2:776215/1‑151 (MQ=255)                    gCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTa                                                                                                                            >  1:561632/1‑150 (MQ=255)                       taagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAatat                                                                                                                        >  1:178815/1‑151 (MQ=255)                        aagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAatat                                                                                                                        <  1:425597/150‑1 (MQ=255)                        aagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCGCCAATCTGCGCTTCCTGGAAGTAATATg                                                                                                                       >  1:301722/1‑151 (MQ=255)                             ataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGgcgc                                                                                                                   <  2:138031/150‑1 (MQ=255)                              taagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCtt                                                                                                                 >  1:309712/1‑151 (MQ=255)                               aagccTTGCAAGAGGTAATAAATCGCTCGCTAAAAAAGAACAACCCCGCCGCCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCtt                                                                                                                 <  2:720226/150‑1 (MQ=255)                                agccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGc                                                                                                               >  2:122263/1‑151 (MQ=255)                                   cTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATc                                                                                                            >  1:582854/1‑151 (MQ=255)                                       ccATAGGTCATAAATCGCTCGCTAAACAAGTACAACCCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAg                                                                                                          <  1:776139/149‑1 (MQ=255)                                           aGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGc                                                                                                    >  2:281508/1‑151 (MQ=255)                                            ggTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGc                                                                                                    <  2:151398/150‑1 (MQ=255)                                                 tAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACttt                                                                                              >  1:714028/1‑151 (MQ=255)                                                  aaaTCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACttt                                                                                              <  2:383770/150‑1 (MQ=255)                                                   aaTCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCTGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGt                                                                                            >  1:703706/1‑151 (MQ=255)                                                             tAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAgg                                                                                  >  2:584978/1‑151 (MQ=255)                                                              aaaCAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGTCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGa                                                                                 <  1:46894/151‑1 (MQ=255)                                                              aaaCAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAg                                                                                   <  2:24689/149‑1 (MQ=255)                                                              aaaCAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGa                                                                                 <  2:647207/151‑1 (MQ=255)                                                                  aaGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACg                                                                             >  2:131656/1‑151 (MQ=255)                                                                  aaGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACg                                                                             >  2:676493/1‑151 (MQ=255)                                                                    gTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTc                                                                           <  2:36354/151‑1 (MQ=255)                                                                        aacaCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCt                                                                          <  2:549060/148‑1 (MQ=255)                                                                          cacCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCa                                                                      <  2:62131/150‑1 (MQ=255)                                                                               ccgTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCaaa                                                                <  2:467333/151‑1 (MQ=255)                                                                                   cGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacga                                                            <  1:158778/151‑1 (MQ=255)                                                                                   cGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacg                                                             >  1:291059/1‑150 (MQ=255)                                                                                       gACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacgaacg                                                         <  2:312291/150‑1 (MQ=255)                                                                                       gACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacgaacg                                                         <  1:708410/150‑1 (MQ=255)                                                                                           ccACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCgt                                                     <  2:135757/150‑1 (MQ=255)                                                                                                      atatCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGgcgc                                         >  2:26707/1‑151 (MQ=255)                                                                                                          cGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGAc                                      >  1:252345/1‑150 (MQ=255)                                                                                                                  tCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTcc                              <  1:630059/150‑1 (MQ=255)                                                                                                                    aGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGc                           <  2:422218/151‑1 (MQ=255)                                                                                                                      gTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTc                         >  1:511686/1‑151 (MQ=255)                                                                                                                          aaCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacgaacg                                                         <  1:119107/115‑1 (MQ=255)                                                                                                                          aaCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacgaacg                                                         >  2:119139/1‑115 (MQ=255)                                                                                                                          aaCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAAAGTCCTGCTCcac                      >  1:424808/1‑150 (MQ=255)                                                                                                                           aCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACaa                    <  2:393913/151‑1 (MQ=255)                                                                                                                              tccatcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACt                  <  2:218554/150‑1 (MQ=255)                                                                                                                               ccatcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTg                <  2:397878/151‑1 (MQ=255)                                                                                                                                     cggcgATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCaa             >  2:750499/1‑148 (MQ=255)                                                                                                                                        cgATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACAt       >  2:477559/1‑151 (MQ=255)                                                                                                                                         gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc      <  1:543595/151‑1 (MQ=255)                                                                                                                                         gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc      >  1:658066/1‑151 (MQ=255)                                                                                                                                          aTCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATcg     >  2:13661/1‑151 (MQ=255)                                                                                                                                             gcgGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATcgccg  >  2:199547/1‑151 (MQ=255)                                                                                                                                             gcgGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATcgcc   >  1:557462/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCG  >  NC_000913/1751094‑1751384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑