Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,773,044	T→C	V178A (GTA→GCA)	ydiN →	putative MFS transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,773,044	0	T	C	100.0%	153.3 / NA	41	V178A (GTA→GCA)	ydiN	putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/19);  total (22/19)

TCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGTACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCGGTGT  >  NC_000913/1772901‑1773179                                                                                                                                                |                                                                                                                                        tCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGAt                                                                                                                                   <  1:636636/150‑1 (MQ=255)  ccGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCa                                                                                                                                 <  2:311680/151‑1 (MQ=255)  ccGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCa                                                                                                                                 <  1:429538/151‑1 (MQ=255)    gAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGGGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCAc                                                                                                                                >  1:256252/1‑150 (MQ=255)      aaGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCt                                                                                                                             <  2:723382/151‑1 (MQ=255)       aGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTTCGGGGATTCTATTTGCACTGATCACGCTg                                                                                                                            >  1:265160/1‑151 (MQ=255)       aGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGGATTCTATTTGCACTGATCACGCt                                                                                                                             >  1:271840/1‑150 (MQ=255)              ggTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGc                                                                                                                        <  1:633926/148‑1 (MQ=255)              ggTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTg                                                                                                                      <  2:549289/150‑1 (MQ=255)                                     aGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCccagcc                                                                                                >  1:638503/1‑149 (MQ=255)                                       cGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGtt                                                                                            >  1:312687/1‑151 (MQ=255)                                          tGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTtggt                                                                                         >  2:120859/1‑151 (MQ=255)                                                cATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGc                                                                                   >  2:691030/1‑151 (MQ=255)                                                         aaaTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTaa                                                                           <  1:320156/150‑1 (MQ=255)                                                         aaaTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAAc                                                                          >  2:344759/1‑151 (MQ=255)                                                         aaaTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAAc                                                                          >  1:189541/1‑151 (MQ=255)                                                         aaaTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAAc                                                                          >  2:189809/1‑151 (MQ=255)                                                          aaTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACt                                                                         >  2:356075/1‑151 (MQ=255)                                                          aaTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACt                                                                         <  2:326574/151‑1 (MQ=255)                                                           aTGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACt                                                                         >  1:633354/1‑150 (MQ=255)                                                             gTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAAt                                                                      >  1:141141/1‑151 (MQ=255)                                                               tCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATg                                                                     <  1:546046/150‑1 (MQ=255)                                                                  aCCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGa                                                                    >  1:684618/1‑148 (MQ=255)                                                                  aCCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGa                                                                    <  2:684692/148‑1 (MQ=255)                                                                   cccAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTa                                                                <  2:732041/151‑1 (MQ=255)                                                                     cAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTAcc                                                              >  2:327506/1‑151 (MQ=255)                                                                                         ttGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCg                                           >  1:605778/1‑150 (MQ=255)                                                                                          tGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGtt                                         >  1:653098/1‑151 (MQ=255)                                                                                              cAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTc                                     >  1:74567/1‑151 (MQ=255)                                                                                                   atatCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTctggct                                <  1:552402/151‑1 (MQ=255)                                                                                                             cGGCTATGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGtt                      >  1:460536/1‑151 (MQ=255)                                                                                                                  aTGGGCTGATTATTCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCa                   <  2:388966/149‑1 (MQ=255)                                                                                                                          ataattCCAGGTATTCTATTTGCACTGATGACACTGATGCTGTTGAAAAGCAAATTCCGCAGCCAGTTGGTGGACGGCAGCGTAACTAATGAATTACCGCAGATGAACAGCAAACCGTTAGTCTGGCTGGAACGTGTTTCATCGGTACt           <  1:304761/146‑1 (MQ=255)                                                                                                                            tattCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGtt        <  2:423676/150‑1 (MQ=255)                                                                                                                            tattCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTc       >  1:179661/1‑151 (MQ=255)                                                                                                                             attCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCg      >  2:53105/1‑151 (MQ=255)                                                                                                                             attCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCg      <  2:509721/151‑1 (MQ=255)                                                                                                                             attCCGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCg      <  2:460587/151‑1 (MQ=255)                                                                                                                                ccGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCGgtg   >  1:488846/1‑151 (MQ=255)                                                                                                                                 cGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCGgtgt  <  2:653156/151‑1 (MQ=255)                                                                                                                                 cGGGTATTCTATTTGCACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCGgtg   <  2:762109/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                        TCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGTACTGATCACGCTGATGCTGTTGAAAAGCAAATTCCCCAGCCAGTTGGTGGACGCCAGCGTAACTAATGAATTACCGCAAATGAACAGCAAACCGTTAGTCTGGCTGGAAGGTGTTTCATCGGTACTGTTCGGTGT  >  NC_000913/1772901‑1773179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑