Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,475	T→C	Y257C (TAC→TGC)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,475	0	T	C	100.0%	158.9 / NA	43	Y257C (TAC→TGC)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

ACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGC  >  NC_000913/1790331‑1790620                                                                                                                                                 |                                                                                                                                                  acaaATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCaa                                                                                                                                             <  1:698469/151‑1 (MQ=255)   aaaTGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGTCAGGTTGATTATCAAAACTGTAACGCCCTTGATGACCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCa                                                                                                                                              <  2:197804/148‑1 (MQ=255)     aTGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGt                                                                                                                                         <  2:715083/151‑1 (MQ=255)        gCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTc                                                                                                                                        >  2:752094/1‑149 (MQ=255)            cAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGcccc                                                                                                                                  >  1:736298/1‑151 (MQ=255)             attGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCca                                                                                                                                 <  1:771175/149‑1 (MQ=255)              aTGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGcccc                                                                                                                                  >  1:651742/1‑149 (MQ=255)                     cgcCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGAc                                                                                                                         >  2:332462/1‑151 (MQ=255)                        cAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACAtg                                                                                                                      <  1:502158/151‑1 (MQ=255)                           aCGCTGTAAATTCCACAACGCGACGGCAGTTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACAtgt                                                                                                                     <  1:23094/149‑1 (MQ=255)                           aCGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACAtgttg                                                                                                                   <  1:444110/151‑1 (MQ=255)                            cGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACAtgttgt                                                                                                                  >  2:406511/1‑151 (MQ=255)                                         acaACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAc                                                                                                     <  1:462033/151‑1 (MQ=255)                                          caACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcc                                                                                                    >  2:500086/1‑151 (MQ=255)                                            aCGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcccc                                                                                                  <  2:342059/151‑1 (MQ=255)                                             cgcgACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcccc                                                                                                  >  1:88611/1‑150 (MQ=255)                                                   ggCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGc                                                                                            >  2:501505/1‑150 (MQ=255)                                                   ggCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCa                                                                                           <  1:570483/151‑1 (MQ=255)                                                      aGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaa                                                                                          >  1:539747/1‑149 (MQ=255)                                                            aTTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGAc                                                                                   <  1:673595/150‑1 (MQ=255)                                                              tATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCg                                                                                 >  1:660186/1‑150 (MQ=255)                                                               aTCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTc                                                                               >  1:263068/1‑151 (MQ=255)                                                                  aaaGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGc                                                                            <  2:128182/151‑1 (MQ=255)                                                                   aaGCTGTAACGCCCTTGATTATCCGAGTGATTCCAAATAAAACCTGGTTCGTAATCGTCAAGAAGACCAAACGGCCCTCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGc                                                                            <  1:248123/150‑1 (MQ=255)                                                                     gCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCAt                                                                         <  1:233647/151‑1 (MQ=255)                                                                       tGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTg                                                                       >  2:237309/1‑151 (MQ=255)                                                                        gTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCAtt                                                                        <  2:40197/149‑1 (MQ=255)                                                                          aaCGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGc                                                                    >  1:112685/1‑151 (MQ=255)                                                                               ccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTa                                                               <  2:261807/151‑1 (MQ=255)                                                                                   gatgatCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAacg                                                            <  1:235767/150‑1 (MQ=255)                                                                                               gATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGc                                               <  1:513492/151‑1 (MQ=255)                                                                                                           aaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCt                                   <  1:250144/151‑1 (MQ=255)                                                                                                              cGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTaa                                 >  1:260301/1‑150 (MQ=255)                                                                                                                ggTTCGTAATCATCAAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAcc                              >  2:596189/1‑151 (MQ=255)                                                                                                                         tcatcaAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGt                     >  2:577299/1‑151 (MQ=255)                                                                                                                             caAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt                 >  2:473940/1‑151 (MQ=255)                                                                                                                             caAGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt                  <  2:513833/150‑1 (MQ=255)                                                                                                                              aaGAAAACCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTg                <  1:406462/151‑1 (MQ=255)                                                                                                                                   aaCCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtc           >  1:341583/1‑151 (MQ=255)                                                                                                                                    aCCAAACGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtc           >  1:275169/1‑150 (MQ=255)                                                                                                                                          cGGCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCt    <  1:30368/151‑1 (MQ=255)                                                                                                                                           ggCCCGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTg   <  2:486375/151‑1 (MQ=255)                                                                                                                                             cccGCAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGc  >  2:527206/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  ACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGC  >  NC_000913/1790331‑1790620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑