Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,796,034	A→G	R637R (CGT→CGC)	pheT ←	phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,796,034	0	A	G	100.0%	147.1 / NA	44	R637R (CGT→CGC)	pheT	phenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

CCACTCCAGTTCGAACACCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCACGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGCAAATCACACCGGCTAAC  >  NC_000913/1795887‑1796172                                                                                                                                                    |                                                                                                                                           ccACTCCAGTTCGAACACCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCgg                                                                                                                                         <  2:108909/151‑1 (MQ=255)  cACTCCAGTTCGAACACCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCgg                                                                                                                                         >  2:102788/1‑150 (MQ=255)          ttCGAACACCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAAcc                                                                                                                                >  2:497713/1‑151 (MQ=255)          ttCGAACACCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAAcc                                                                                                                                >  1:487937/1‑151 (MQ=255)               acacCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCAtt                                                                                                                           >  1:395844/1‑151 (MQ=255)                           gACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGt                                                                                                               >  2:567871/1‑151 (MQ=255)                               gTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAgg                                                                                                           >  2:47638/1‑151 (MQ=255)                                ttAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTGCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCTGAACTCAACCTCATTCAGTTTACCGGTCacg                                                                                                           <  1:14218/150‑3 (MQ=255)                                  aaGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTc                                                                                                         <  2:721886/150‑1 (MQ=255)                                  aaGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCg                                                                                                        <  1:239716/151‑1 (MQ=255)                                     aTCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCgaga                                                                                                     <  2:506720/151‑1 (MQ=255)                                       ccAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAAc                                                                                                   >  2:129199/1‑151 (MQ=255)                                         aGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACgg                                                                                                 <  1:712044/151‑1 (MQ=255)                                             tACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGAtt                                                                                              <  2:398821/150‑1 (MQ=255)                                             tACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTc                                                                                             >  1:299682/1‑151 (MQ=255)                                              aCGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCa                                                                                            <  1:184672/151‑1 (MQ=255)                                               cGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCaa                                                                                           <  2:299715/151‑1 (MQ=255)                                               cGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCa                                                                                            >  2:402831/1‑150 (MQ=255)                                                gTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCa                                                                                            >  2:364496/1‑149 (MQ=255)                                                       ttCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCt                                                                                   >  1:488426/1‑151 (MQ=255)                                                           ggATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTc                                                                                >  2:527170/1‑150 (MQ=255)                                                            gATTAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCTGATTGCCCCGTATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCaa                                                                              <  2:634894/151‑1 (MQ=255)                                                                 acaacCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCAt                                                                         <  1:558195/151‑1 (MQ=255)                                                                   aacCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAg                                                                       <  2:291025/151‑1 (MQ=255)                                                                    acCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGa                                                                      <  2:68582/151‑1 (MQ=255)                                                                     ccccAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGa                                                                      >  2:710261/1‑150 (MQ=255)                                                                      cccAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGaa                                                                     >  1:35698/1‑150 (MQ=255)                                                                               aCCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGtc                                                           <  1:699565/151‑1 (MQ=255)                                                                                ccAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGtc                                                           >  2:356091/1‑150 (MQ=255)                                                                                 cAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGtctc                                                         <  2:395893/151‑1 (MQ=255)                                                                                     aCGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCtttt                                                     <  2:257916/151‑1 (MQ=255)                                                                                          cACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCa                                                 <  1:64895/150‑1 (MQ=255)                                                                                            ccTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAgg                                               <  2:176335/150‑1 (MQ=255)                                                                                                   gATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTg                                       <  2:63131/151‑1 (MQ=255)                                                                                                      aaaTCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGctc                                    >  1:442576/1‑151 (MQ=255)                                                                                                       aaTCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGctct                                   >  2:191575/1‑151 (MQ=255)                                                                                                          cGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCg                                <  1:51083/151‑1 (MQ=255)                                                                                                              gCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTa                              <  2:631523/149‑1 (MQ=255)                                                                                                                        ccGGATGCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGc                   >  2:89065/1‑150 (MQ=255)                                                                                                                              gCAGTGCCGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTGACGGTTACCGCAAATcac            >  1:274998/1‑151 (MQ=255)                                                                                                                               cAGTGCCGGAATCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGCAAATca             >  1:411380/1‑149 (MQ=255)                                                                                                                                     cGGATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGCAAATCACACCGGCt     <  1:702907/151‑1 (MQ=255)                                                                                                                                      ggATTCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGCAAATCACACCGGCTa    <  1:529363/151‑1 (MQ=255)                                                                                                                                         ttCGCTTCTGCGCGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGCAAATCACACCGGCTAAc  >  2:116124/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                           CCACTCCAGTTCGAACACCAGAGTGCGACCGTTAAGATCCAGTTTACGTTCCAGTTCAGGATGAACAACCCCAACAAAACCAATACGTTCACCTTTCAGATAAATCGCTGCGGATTGCCCCGGATGCAGTGCCGGATTCGCTTCTGCACGGAACTCAACCTCATTCAGTTTACCGGTCAGGTCGAGAACGGATTCAAGATCGCCTTTCAAATCATAGAAATCAACGGTCTCTTTTGCCAGGTTCCAGTGCTCTTCGTAACGGTTACCGCAAATCACACCGGCTAAC  >  NC_000913/1795887‑1796172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑