Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,824,020	T→C	L169P (CTG→CCG)	cho →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,824,020	0	T	C	100.0%	186.5 / NA	52	L169P (CTG→CCG)	cho	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/32);  total (20/32)

ATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTG  >  NC_000913/1823874‑1824167                                                                                                                                                   |                                                                                                                                                    aTTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCccgctg                                                                                                                                                 <  1:663367/151‑1 (MQ=255)    aaaTGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTggg                                                                                                                                               >  2:776353/1‑150 (MQ=255)        gAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAGCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTg                                                                                                                                           <  1:508930/150‑1 (MQ=255)          aaaGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGa                                                                                                                                         >  2:416259/1‑150 (MQ=255)           aaGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAAc                                                                                                                                       >  1:694827/1‑151 (MQ=255)               gcgTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGtt                                                                                                                                   <  1:685961/151‑1 (MQ=255)                cgTCGCTGTGGTGTATGCCAAAGAGGGGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGtt                                                                                                                                   >  1:488412/1‑150 (MQ=255)                   cGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAg                                                                                                                                <  2:454113/150‑1 (MQ=255)                     aTGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTcg                                                                                                                             >  2:678982/1‑151 (MQ=255)                       gtggtgTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTcgcg                                                                                                                           <  1:762760/151‑1 (MQ=255)                        tggtgTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCgg                                                                                                                          >  1:461352/1‑151 (MQ=255)                             tATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTg                                                                                                                     >  2:415564/1‑151 (MQ=255)                                  cAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATg                                                                                                                 <  2:680980/150‑1 (MQ=255)                                  cAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGt                                                                                                                >  1:237661/1‑151 (MQ=255)                                   aaaGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGtt                                                                                                               <  1:416208/151‑1 (MQ=255)                                      gagGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTc                                                                                                            <  2:382257/151‑1 (MQ=255)                                             aTTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgc                                                                                                     <  2:384990/151‑1 (MQ=255)                                             aTTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgc                                                                                                     >  1:365962/1‑151 (MQ=255)                                                tttCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAgcgc                                                                                                   <  1:677744/150‑1 (MQ=255)                                                  tCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTa                                                                                                 <  2:278690/150‑1 (MQ=255)                                                     cGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaaa                                                                                              <  1:461039/150‑1 (MQ=255)                                                     cGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAc                                                                                             >  2:503588/1‑151 (MQ=255)                                                      gAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                                                                            >  2:17492/1‑151 (MQ=255)                                                             cAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                                                                                     <  1:171383/151‑1 (MQ=255)                                                                  tGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGc                                                                                >  1:4087/1‑151 (MQ=255)                                                                   gTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCa                                                                               <  2:341386/151‑1 (MQ=255)                                                                        gCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctg                                                                          <  1:382891/151‑1 (MQ=255)                                                                         ccTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctgc                                                                         <  2:747484/151‑1 (MQ=255)                                                                         ccTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctg                                                                          >  2:650891/1‑150 (MQ=255)                                                                            gTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCgg                                                                       <  2:143411/150‑1 (MQ=255)                                                                                           cgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATg                                                         >  2:278278/1‑149 (MQ=255)                                                                                               gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGaa                                                    <  1:459900/150‑1 (MQ=255)                                                                                               gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAAc                                                   <  1:192089/151‑1 (MQ=255)                                                                                               gCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAAc                                                   <  1:450588/151‑1 (MQ=255)                                                                                                      aaGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCt                                            >  2:384854/1‑151 (MQ=255)                                                                                                      aaGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCt                                            >  1:774845/1‑151 (MQ=255)                                                                                                       aGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTa                                           <  2:642886/151‑1 (MQ=255)                                                                                                       aGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTa                                           <  2:94776/151‑1 (MQ=255)                                                                                                           ttGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCt                                       >  2:605906/1‑151 (MQ=255)                                                                                                            tGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCt                                       <  2:358218/150‑1 (MQ=255)                                                                                                            tGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCt                                       <  1:642407/150‑1 (MQ=255)                                                                                                             gCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTg                                     <  2:697602/151‑1 (MQ=255)                                                                                                                gACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTgcgc                                  >  2:535365/1‑151 (MQ=255)                                                                                                                      cGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGtct                            <  2:710988/151‑1 (MQ=255)                                                                                                                           atgaACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGa                       <  1:302031/151‑1 (MQ=255)                                                                                                                              aaCAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCg                    >  2:30578/1‑151 (MQ=255)                                                                                                                               aCAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGt                   <  2:421085/151‑1 (MQ=255)                                                                                                                                        ttGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCggg            <  2:209194/149‑1 (MQ=255)                                                                                                                                        ttGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGggt           <  1:641538/150‑1 (MQ=255)                                                                                                                                        ttGTTATGGCCCGCGGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGACATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGggt           <  1:684385/150‑1 (MQ=255)                                                                                                                                            tATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGgtggtg       >  2:324951/1‑150 (MQ=255)                                                                                                                                               ggCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGtt   <  1:536445/151‑1 (MQ=255)                                                                                                                                                gCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTg  <  2:570014/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    ATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTGTGTTG  >  NC_000913/1823874‑1824167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑