Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,882	A→G	P197P (CCT→CCC)	mipA ←	scaffolding protein for murein synthesizing machinery

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,882	0	A	G	100.0%	142.4 / NA	41	P197P (CCT→CCC)	mipA	scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/18);  total (23/18)

TGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGT  >  NC_000913/1865734‑1866027                                                                                                                                                     |                                                                                                                                                  tGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAggg                                                                                                                                                 >  2:191665/1‑151 (MQ=255)  gTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAgggg                                                                                                                                                <  2:385272/151‑1 (MQ=255)    aGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGc                                                                                                                                               <  2:40466/150‑1 (MQ=255)      gTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTcc                                                                                                                                            >  1:530038/1‑151 (MQ=255)       tGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTcc                                                                                                                                            >  1:232154/1‑150 (MQ=255)         aTCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGc                                                                                                                                         >  1:666367/1‑151 (MQ=255)         aTCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGc                                                                                                                                         >  2:382717/1‑151 (MQ=255)           cccGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTg                                                                                                                                       <  2:763106/151‑1 (MQ=255)           cccGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTg                                                                                                                                       >  2:728475/1‑151 (MQ=255)            ccGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGt                                                                                                                                      >  2:164162/1‑151 (MQ=255)              ggTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCg                                                                                                                                    <  1:134551/151‑1 (MQ=255)                         ggCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGCGGCTCCAGCTGTCGTTCGGGTtat                                                                                                                          >  2:148472/1‑150 (MQ=255)                          gCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAg                                                                                                                        <  2:490438/151‑1 (MQ=255)                                  aGGATTTATCCACCATCGGGCTGTCAGTAACCTCCTCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGCGGAAGTTGTAGCTGGCGCTCAGCTCCAGGCAGGGGCTCCAGCTGTCCTTCGGGTTATAGCCACGc                                                                                                                  >  1:569106/1‑149 (MQ=255)                                     aTTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGAcc                                                                                                             >  1:524870/1‑151 (MQ=255)                                     aTTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGAcc                                                                                                             <  1:644493/151‑1 (MQ=255)                                       ttATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGc                                                                                                           >  1:312165/1‑151 (MQ=255)                                             accaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGCGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCg                                                                                                       >  1:418320/1‑149 (MQ=255)                                              ccaTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGc                                                                                                    >  1:243104/1‑151 (MQ=255)                                               caTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCg                                                                                                   <  1:173429/151‑1 (MQ=255)                                                  cgcgCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGAc                                                                                                >  1:351977/4‑151 (MQ=255)                                                  cGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGa                                                                                                 <  2:227255/150‑1 (MQ=255)                                                  cGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGAc                                                                                                <  2:53593/151‑1 (MQ=255)                                                  agggCTGTCAGTAACTTCCTCAGACAGACGGGTGTAGCGCGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGCTATAGCCACGCAGACCGCTGCGAGCGGa                                                                                                 >  1:596538/2‑150 (MQ=255)                                                   gggCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGAct                                                                                               <  2:266780/151‑1 (MQ=255)                                                        gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCttt                                                                                           <  1:745802/150‑1 (MQ=255)                                                          cAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTgc                                                                                         <  2:524922/150‑1 (MQ=255)                                                                   catcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGcc                                                                               >  2:132781/1‑151 (MQ=255)                                                                      cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCat                                                                             <  1:508308/150‑1 (MQ=255)                                                                       agacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCataa                                                                           <  1:423006/151‑1 (MQ=255)                                                                         acagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCataat                                                                          >  1:181278/1‑150 (MQ=255)                                                                             acGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                                                     >  1:433609/1‑151 (MQ=255)                                                                                     aGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCt                                                              <  1:110778/150‑1 (MQ=255)                                                                                            ggTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCg                                                      <  2:684399/151‑1 (MQ=255)                                                                                             gTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCTTCTCCAGGTAGGGGCTCCTGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGCGCGTCATCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGc                                                     >  1:125943/1‑151 (MQ=255)                                                                                                     aaCACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCAc                                             <  2:280827/151‑1 (MQ=255)                                                                                                     aaCACTACAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCAc                                             >  1:365667/1‑151 (MQ=255)                                                                                                        acTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGc                                          >  1:144312/1‑151 (MQ=255)                                                                                                          tCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGcac                                        >  1:228780/1‑151 (MQ=255)                                                                                                                           ttGTAGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTc                        >  1:506101/1‑150 (MQ=255)                                                                                                                               aGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACgg                    <  2:312198/150‑1 (MQ=255)                                                                                                                               aGCTGGCGCTCAGCTCCAGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGt                   >  1:49047/1‑151 (MQ=255)                                                                                                                                                aGGTAGGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgtgt  <  2:329658/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGT  >  NC_000913/1865734‑1866027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑