Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,010,754	T→C	V52A (GTA→GCA)	yedL →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,010,754	0	T	C	100.0%	185.2 / NA	48	V52A (GTA→GCA)	yedL	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/25);  total (23/25)

AAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGCC  >  NC_000913/2010613‑2010899                                                                                                                                              |                                                                                                                                                  aaaCAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAgg                                                                                                                                          >  1:594185/1‑151 (MQ=255)         gatCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGaacaa                                                                                                                                   <  2:259147/150‑1 (MQ=255)              cACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGc                                                                                                                              <  2:739085/150‑1 (MQ=255)                cccATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAgg                                                                                                                           >  1:245821/1‑151 (MQ=255)                cccATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAgg                                                                                                                           <  1:641527/151‑1 (MQ=255)                     ccAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTg                                                                                                                      >  2:581632/1‑151 (MQ=255)                      cagcagTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTgg                                                                                                                     >  1:682533/1‑151 (MQ=255)                          agTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTgcg                                                                                                                 >  1:283127/1‑151 (MQ=255)                                aGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAg                                                                                                           <  2:406199/151‑1 (MQ=255)                                 gCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGt                                                                                                          >  1:369053/1‑151 (MQ=255)                                      aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGTCAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGt                                                                                                          <  1:348446/146‑1 (MQ=255)                                      aGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTg                                                                                                     <  1:232472/151‑1 (MQ=255)                                         ggCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGga                                                                                                   <  2:681747/150‑1 (MQ=255)                                            ttACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGgatgat                                                                                               >  1:225839/1‑151 (MQ=255)                                               ccATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATaa                                                                                             <  1:209795/150‑1 (MQ=255)                                                cATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAac                                                                                           <  2:354231/151‑1 (MQ=255)                                                      tCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCg                                                                                     >  2:719226/1‑151 (MQ=255)                                                       ccGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGc                                                                                      <  1:206164/149‑1 (MQ=255)                                                        cGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAg                                                                                   <  2:766706/151‑1 (MQ=255)                                                         ggCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGc                                                                                  <  2:117535/151‑1 (MQ=255)                                                          gCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCt                                                                                 <  2:225872/151‑1 (MQ=255)                                                                 gcagcaGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATc                                                                           >  1:350565/1‑150 (MQ=255)                                                                   agcagTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAAt                                                                        <  2:594242/151‑1 (MQ=255)                                                                    gcagTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATg                                                                       >  1:678590/1‑151 (MQ=255)                                                                        tctcCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGt                                                                    <  1:717383/150‑1 (MQ=255)                                                                             ccccGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACcgcg                                                              >  1:694812/1‑151 (MQ=255)                                                                                  aaaGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCaa                                                          >  2:631133/1‑150 (MQ=255)                                                                                    aGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAatt                                                       <  2:230296/151‑1 (MQ=255)                                                                                      cAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAattat                                                     <  2:299874/151‑1 (MQ=255)                                                                                        gTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTAttt                                                   <  2:427567/151‑1 (MQ=255)                                                                                            tGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTTGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTAcg                                                <  2:352026/150‑1 (MQ=255)                                                                                                    aTGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGgtgt                                       <  2:151547/151‑1 (MQ=255)                                                                                                    aTGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGgtgt                                       >  2:499347/1‑151 (MQ=255)                                                                                                     tgtgCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTc                                      >  1:235753/1‑151 (MQ=255)                                                                                                        gCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGcc                                   >  2:747169/1‑151 (MQ=255)                                                                                                           aaaaTTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGt                                >  2:354730/1‑151 (MQ=255)                                                                                                           aaaaTTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGt                                <  2:54894/151‑1 (MQ=255)                                                                                                           aaaaTTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGt                                <  1:450025/151‑1 (MQ=255)                                                                                                             aaTTACGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCt                              >  1:371918/1‑151 (MQ=255)                                                                                                                 aCGTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGAtt                          <  2:754870/151‑1 (MQ=255)                                                                                                                   gTAACCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGAtttt                        <  1:659657/151‑1 (MQ=255)                                                                                                                     aaCCCGACAGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTAc                      >  1:232497/1‑151 (MQ=255)                                                                                                                             aGTGACATTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACAtt              <  2:99900/151‑1 (MQ=255)                                                                                                                                   aTTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGc          >  1:374285/1‑149 (MQ=255)                                                                                                                                   aTTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGc          >  2:35253/1‑149 (MQ=255)                                                                                                                                   aTTCTGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGc          >  2:320106/1‑149 (MQ=255)                                                                                                                                       tGGTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTc     >  1:435326/1‑150 (MQ=255)                                                                                                                                         gTCAGCATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGcc  >  2:344739/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                  AAACAGATGATCTCACCCATCCAGCAGTGCAAGCATTAGTGGCTTACCATATTTCCGGCATGCTGCAGCAGTCTCCCCCCGAAAGCAGTCATGCTTTAGATGTGCAAAAATTACGTAACCCGACAGTGACATTCTGGTCAGTATGGGAAGGCGAACAACTCGCAGGAATTGGTGCGCTGAAGTTGCTGGATGATAAACACGGCGAGCTGAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGCC  >  NC_000913/2010613‑2010899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑