Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,091,655	G→A	A187T (GCA→ACA)	hisD →	bifunctional histidinal dehydrogenase/ histidinol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,091,655	0	G	A	100.0%	193.4 / NA	51	A187T (GCA→ACA)	hisD	bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/13);  total (38/13)

TCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCC  >  NC_000913/2091512‑2091802                                                                                                                                                |                                                                                                                                                    tctcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGc                                                                                                                                              >  1:145792/1‑151 (MQ=255) tctcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGc                                                                                                                                              >  2:482280/1‑151 (MQ=255)  ctcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGcc                                                                                                                                             >  2:135872/1‑151 (MQ=255)   tcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGcc                                                                                                                                             >  2:2677/1‑150 (MQ=255)          tATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGc                                                                                                                                     >  1:405350/1‑151 (MQ=255)           aTTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGc                                                                                                                                     >  1:294109/1‑150 (MQ=255)            ttAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCAc                                                                                                                                   >  2:390982/1‑151 (MQ=255)              aaTGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTg                                                                                                                                 >  2:58102/1‑151 (MQ=255)                    ggCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGttt                                                                                                                           >  2:228063/1‑151 (MQ=255)                     gCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTg                                                                                                                          <  2:65256/151‑1 (MQ=255)                       gACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGt                                                                                                                        <  2:390875/151‑1 (MQ=255)                           ccGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACgg                                                                                                                    >  1:776998/1‑151 (MQ=255)                              gCGAGTATTGCGGGCTGTAAAAAATTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGATGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGaa                                                                                                                  <  1:483059/150‑1 (MQ=255)                               cGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGTCGGCACACAGGCCTTTGCCGCACTGGCGTTTGGTACGGAATc                                                                                                                >  2:553477/1‑151 (MQ=255)                                      tGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGcc                                                                                                          >  2:232590/1‑150 (MQ=255)                                                aaaaaaGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGAc                                                                                                >  2:85690/1‑150 (MQ=255)                                                     aGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATc                                                                                          >  1:188781/1‑151 (MQ=255)                                                        ggtgCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTc                                                                                       <  1:624180/151‑1 (MQ=255)                                                             tgtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGcc                                                                                  >  2:305563/1‑151 (MQ=255)                                                              gtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGc                                                                                   <  2:693990/149‑1 (MQ=255)                                                               tgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCgg                                                                                >  1:169485/1‑151 (MQ=255)                                                                 ctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCggg                                                                               >  1:571525/1‑150 (MQ=255)                                                                     ccgccgccgATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAAc                                                                           <  1:416109/150‑1 (MQ=255)                                                                       gccgccgATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGcc                                                                        >  2:210431/1‑151 (MQ=255)                                                                         cgccgATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCtt                                                                      <  1:68226/151‑1 (MQ=255)                                                                            cgATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGt                                                                   >  1:276391/1‑151 (MQ=255)                                                                             gATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTc                                                                  >  1:387588/1‑151 (MQ=255)                                                                                 gCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCAGCGTCATACATGCCATTGCCGCCCTGTCGTTCGGTCCGCAATCTGTGCCAAAAGTTGACAAAATCTTCGTCCCGGGTAACGCCTTGATCACCg                                                              <  1:122391/151‑1 (MQ=255)                                                                                  ccGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCg                                                              >  2:90405/1‑150 (MQ=255)                                                                                  ccGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCg                                                              >  1:278981/1‑150 (MQ=255)                                                                                     aTGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGc                                                          >  1:283736/1‑151 (MQ=255)                                                                                      tGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCg                                                         >  2:626435/1‑151 (MQ=255)                                                                                        agaTCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCg                                                         >  1:178130/1‑149 (MQ=255)                                                                                         gaTCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGaa                                                       >  1:68686/1‑150 (MQ=255)                                                                                          aTCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAAc                                                     >  1:232159/1‑151 (MQ=255)                                                                                           tCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACg                                                    >  1:636229/1‑151 (MQ=255)                                                                                               ttATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCa                                                 <  1:376300/150‑1 (MQ=255)                                                                                                   gcggcgCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTg                                             <  1:456565/150‑1 (MQ=255)                                                                                                        gcAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGcc                                         >  1:668399/1‑149 (MQ=255)                                                                                                        gcAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGcc                                         >  1:305469/1‑149 (MQ=255)                                                                                                         cAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGc                                      >  2:641379/1‑151 (MQ=255)                                                                                                               tgCGGTGTGCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTgg                                >  2:389271/1‑151 (MQ=255)                                                                                                                    agtgCAGGACGTGTTTAACGTCGGCGGCACAAAGGCCATTGCCGCACTGGCGTGTGGTACGGAACCTGTGCCAAAAGTGGAAAAAATATTCGGGCCGGGTAACGCCTTTGTCACAGAAGCGAAACAACAGGTGAGTCAGCGTCTGGACGGt                           >  1:699557/2‑151 (MQ=255)                                                                                                                      tgCAGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTg                          >  1:487365/1‑150 (MQ=255)                                                                                                                         aGGACGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTgcggc                      <  2:232192/151‑1 (MQ=255)                                                                                                                            aCGTGTTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgat                   >  2:647214/1‑151 (MQ=255)                                                                                                                              gtgtTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgatcg                 >  1:185281/1‑151 (MQ=255)                                                                                                                                gtTTAACGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgatcgat               <  2:538424/151‑1 (MQ=255)                                                                                                                                    aaCGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAg                                           <  2:230412/119‑1 (MQ=255)                                                                                                                                    aaCGTCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAg                                           >  1:230379/1‑119 (MQ=255)                                                                                                                                        tCGGCGGCACACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGCGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGc       >  2:567177/1‑151 (MQ=255)                                                                                                                                            cggcaCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGc   >  1:169973/1‑151 (MQ=255)                                                                                                                                             ggcaCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGcc  <  1:753838/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    TCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCC  >  NC_000913/2091512‑2091802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑