Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,152,439	C→T	G12R (GGA→AGA)	yegK ←	ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,152,439	0	C	T	100.0%	131.3 / NA	35	G12R (GGA→AGA)	yegK	ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

GAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATC  >  NC_000913/2152293‑2152584                                                                                                                                                   |                                                                                                                                                  gAGCATCGCGCCTTCGCCACCCGGCGAGACACTGCCTGCGCCATCAGCGACAAATCCCGACAGTAATGGCTGCTGGTCATTTAACCAGGCAATCTGCGTCTGGCAGGCACCGTGACAAGGCAAGTCCGCGCTGCTGTGCGGCGTTCTAAct                                                                                                                                               <  1:304037/151‑2 (MQ=255)       cgcgCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGa                                                                                                                                          <  2:363951/150‑1 (MQ=255)              tCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAg                                                                                                                                   <  1:13131/150‑1 (MQ=255)                        gCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGcc                                                                                                                         <  1:105402/150‑1 (MQ=255)                                  gcctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACt                                                                                                               <  1:196609/150‑1 (MQ=255)                                      gcgcCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGAc                                                                                                            <  2:286063/149‑1 (MQ=255)                                        gcCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTcc                                                                                                        >  1:458843/1‑151 (MQ=255)                                          cATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTcc                                                                                                        >  1:174464/1‑149 (MQ=255)                                          cATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAg                                                                                                      <  1:385434/151‑1 (MQ=255)                                            tCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGcc                                                                                                    >  1:579468/1‑151 (MQ=255)                                                        aCCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTc                                                                                        >  2:723861/1‑151 (MQ=255)                                                            actataatGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAg                                                                                     >  1:726095/4‑150 (MQ=255)                                                             cAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGc                                                                                    >  1:622908/1‑150 (MQ=255)                                                               ataatGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGcaca                                                                                 <  1:131880/151‑1 (MQ=255)                                                                        gctgGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGtt                                                                        >  1:203267/1‑151 (MQ=255)                                                                           ggTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTccc                                                                     >  2:52194/1‑151 (MQ=255)                                                                                tttAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCg                                                                 >  1:531434/1‑150 (MQ=255)                                                                                 ttAACCAGGCAATCTGCATCTGGCAGGCATCCTGACACGGCAAGTCCTCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACAAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTg                                                               >  1:432607/1‑151 (MQ=255)                                                                                  tAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTgg                                                              >  1:71791/1‑151 (MQ=255)                                                                                           aaTCTGCATCTGGCAGGCCTCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTGGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGTCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGaa                                                      >  1:484948/1‑150 (MQ=255)                                                                                            aTCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAAc                                                    >  2:52319/1‑151 (MQ=255)                                                                                               tGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCg                                                  >  2:606846/1‑150 (MQ=255)                                                                                                   tCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACg                                              >  1:31553/1‑150 (MQ=255)                                                                                                      ggcaggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACg                                              <  1:655138/147‑1 (MQ=255)                                                                                                                       ggCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCt                         <  2:464127/151‑1 (MQ=255)                                                                                                                          aaGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTaaa                      <  2:711591/151‑1 (MQ=255)                                                                                                                          aaGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTaa                       >  2:640087/1‑150 (MQ=255)                                                                                                                              ccGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGt                  <  2:55707/151‑1 (MQ=255)                                                                                                                               cgcgCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGtt                 <  1:307238/151‑1 (MQ=255)                                                                                                                                 cgcTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTc                >  2:718195/1‑150 (MQ=255)                                                                                                                                    tGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACgg            >  1:588713/1‑151 (MQ=255)                                                                                                                                     gATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACgg            <  2:123904/150‑1 (MQ=255)                                                                                                                                     gATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACg             <  2:218954/149‑1 (MQ=255)                                                                                                                                       tgtgCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAAt         >  1:192672/1‑151 (MQ=255)                                                                                                                                               gTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTGGGCGCTTCCAGCACAACTTCCGTGCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATc  <  1:135537/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATC  >  NC_000913/2152293‑2152584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑