Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,182,120	A→G	V221A (GTG→GCG)	yegW ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,182,120	0	A	G	100.0%	148.8 / NA	41	V221A (GTG→GCG)	yegW	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/21);  total (20/21)

TGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGAAATCCCG  >  NC_000913/2181974‑2182266                                                                                                                                                   |                                                                                                                                                   tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGctt                                                                                                                                                 >  2:737679/1‑150 (MQ=255)  gCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGcttgc                                                                                                                                               <  1:317562/151‑1 (MQ=255)       ggCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGa                                                                                                                                           <  2:504817/150‑1 (MQ=255)       ggCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGAt                                                                                                                                          <  1:71207/151‑1 (MQ=255)         cgtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCa                                                                                                                                        <  1:26720/151‑1 (MQ=255)           tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCAcc                                                                                                                                      >  1:361738/1‑151 (MQ=255)                      cGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTAt                                                                                                                           <  1:161294/151‑1 (MQ=255)                       gggCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTAtt                                                                                                                          >  1:52237/1‑151 (MQ=255)                         gCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTAtttt                                                                                                                        <  2:183088/151‑1 (MQ=255)                          cATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTg                                                                                                                       <  2:422415/151‑1 (MQ=255)                                ggCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                                                                                                                 <  1:763777/151‑1 (MQ=255)                                      gATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTg                                                                                                            >  1:242320/1‑150 (MQ=255)                                          gtgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAatat                                                                                                       <  1:195720/151‑1 (MQ=255)                                            gcgcCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATAtg                                                                                                      <  1:536593/150‑1 (MQ=255)                                                   cgcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGa                                                                                               >  2:152999/1‑150 (MQ=255)                                                    gcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGaa                                                                                              >  1:688684/1‑150 (MQ=255)                                                             actactCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatc                                                                                    >  1:298153/1‑151 (MQ=255)                                                              ctactCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatcc                                                                                   >  1:573629/1‑151 (MQ=255)                                                                actCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccg                                                                                  <  1:365337/150‑1 (MQ=255)                                                                    ctcGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccggcatc                                                                             >  1:124855/1‑151 (MQ=255)                                                                        cacacaAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGATTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCggg                                                                         >  2:636089/1‑151 (MQ=255)                                                                              aaaaCGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTaa                                                                   <  1:742592/151‑1 (MQ=255)                                                                                   gTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCgg                                                              >  1:70296/1‑151 (MQ=255)                                                                                   gTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCgg                                                              <  2:140064/151‑1 (MQ=255)                                                                                   gTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCgg                                                              <  2:298186/151‑1 (MQ=255)                                                                                          tCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAAcgcg                                                       >  1:635806/1‑151 (MQ=255)                                                                                                cGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGtt                                                 >  1:212425/1‑151 (MQ=255)                                                                                                    aGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGCgg                                             >  1:463734/1‑151 (MQ=255)                                                                                                    aGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCAACGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTAAGTTGTgg                                             >  1:511607/1‑151 (MQ=255)                                                                                                          tAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAAt                                       >  1:66446/1‑151 (MQ=255)                                                                                                           aaTGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTaaa                                        <  1:381705/149‑1 (MQ=255)                                                                                                              gCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAAtgtg                                    <  2:174418/150‑1 (MQ=255)                                                                                                                   aCTCAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTgctggct                              >  1:121397/1‑151 (MQ=255)                                                                                                                      cAATAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTgctggctg                             <  2:506129/149‑1 (MQ=255)                                                                                                                       aaTAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCg                           <  1:666648/150‑1 (MQ=255)                                                                                                                         tAGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGa                         >  1:426016/1‑150 (MQ=255)                                                                                                                          aGGCCGTTGCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGaaa                       <  1:23247/151‑1 (MQ=255)                                                                                                                                  gCTGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATa               <  2:606399/151‑1 (MQ=255)                                                                                                                                    tGTTGGTCAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATaa              >  2:642546/1‑150 (MQ=255)                                                                                                                                           cAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATTCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGAAAt      >  1:767222/1‑151 (MQ=255)                                                                                                                                           cAAGCGCCGCTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGAAAt      <  2:241635/151‑1 (MQ=255)                                                                                                                                               cgccgcTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGAAATCCCg  <  2:434691/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCGCTAACGCGGGAACGCGTACGTTGTGGGTAAATGTGCTGGCTGCGGAAATAGTCATAAAGTGAAATCCCG  >  NC_000913/2181974‑2182266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑