Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,191,240 T→C K136E (AAG→GAG)  yehC ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,191,2400TC96.2% 182.5 / ‑1.5 53K136E (AAG→GAG) yehCputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (23/28);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTGAGTCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGG  >  NC_000913/2191099‑2191378
                                                                                                                                             |                                                                                                                                          
cTGAGTCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTg                                                                                                                                   >  2:285184/1‑151 (MQ=255)
        ttttttATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGAc                                                                                                                           >  1:558594/1‑151 (MQ=255)
              aTCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTAttt                                                                                                                      <  1:622961/150‑1 (MQ=255)
                 aCCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTtggtg                                                                                                                  >  2:337801/1‑151 (MQ=255)
                  ccAACCCGTTGCCACTCCGATTTACCACCAATTTTTTCAATGTCGCTTCATCTACCGCAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCTCCTTCCTGCTCTTGACTATTtgaggg                                                                                                                 >  1:410215/1‑147 (MQ=255)
                  ccAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTtggtgg                                                                                                                 >  1:90324/1‑151 (MQ=255)
                     aaCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAat                                                                                                              >  2:291966/1‑151 (MQ=255)
                     aaCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAat                                                                                                              <  1:688732/151‑1 (MQ=255)
                      aCCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAata                                                                                                             <  1:396373/151‑1 (MQ=255)
                       ccATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCGCCTTCTTGCTCTGGACTATTTGGTGGAatat                                                                                                            <  2:753899/151‑1 (MQ=255)
                           tGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATcc                                                                                                          >  2:123499/1‑149 (MQ=255)
                            gCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAg                                                                                                        <  2:391561/150‑1 (MQ=255)
                            gCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGa                                                                                                       <  1:173295/151‑1 (MQ=255)
                             ccACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGaa                                                                                                      <  2:571514/151‑1 (MQ=255)
                              cACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAAc                                                                                                     <  1:23960/151‑1 (MQ=255)
                                  gCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATCCCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACAtt                                                                                                  <  2:179670/150‑1 (MQ=255)
                                  gCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACAtt                                                                                                  <  2:310009/150‑1 (MQ=255)
                                  gCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTc                                                                                                 <  1:291933/151‑1 (MQ=255)
                                       ttACCAGCAATTTTTTAAATGTCTCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTCCCTTCTTGCTCTTGACTATTTTGTGGAATATCCTGAACATTCAGAt                                                                                             <  1:82085/150‑1 (MQ=255)
                                       ttACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGAt                                                                                             >  2:754724/1‑150 (MQ=255)
                                         aCCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATaa                                                                                           >  2:533508/1‑150 (MQ=255)
                                            aGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATaaaaaa                                                                                       <  2:416986/151‑1 (MQ=255)
                                              cAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATg                                                                                     >  2:549091/1‑151 (MQ=255)
                                                aTTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGct                                                                                   <  1:64259/151‑1 (MQ=255)
                                                 ttttttAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGctt                                                                                  >  2:568969/1‑151 (MQ=255)
                                                   ttttAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGctttc                                                                                >  1:764082/1‑151 (MQ=255)
                                                     ttAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGctttctt                                                                              >  2:559033/1‑151 (MQ=255)
                                                      tAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGctttctt                                                                              <  2:561810/150‑1 (MQ=255)
                                                        aaTGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTttat                                                                           <  2:546708/151‑1 (MQ=255)
                                                         aTGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTttatt                                                                          <  2:443726/151‑1 (MQ=255)
                                                           gTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAg                                                                        <  1:389230/151‑1 (MQ=255)
                                                                      taCCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGttt                                                              <  1:754649/150‑1 (MQ=255)
                                                                        ccGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTAtt                                                           <  2:646626/151‑1 (MQ=255)
                                                                          ggAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCg                                                         <  2:440011/151‑1 (MQ=255)
                                                                            aGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGc                                                       >  1:515514/1‑151 (MQ=255)
                                                                                aTACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATaa                                                   <  2:626775/151‑1 (MQ=255)
                                                                                       cTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGa                                             <  2:186963/150‑1 (MQ=255)
                                                                                       cTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGAt                                            >  2:134665/1‑151 (MQ=255)
                                                                                        tGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGAtt                                           >  1:526234/1‑151 (MQ=255)
                                                                                         ggCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGAtt                                           <  2:69939/150‑1 (MQ=255)
                                                                                          gCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGAtttt                                         >  2:460017/1‑151 (MQ=255)
                                                                                          gCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGAtt                                           <  1:194450/149‑1 (MQ=255)
                                                                                            cGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTTGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTa                                       <  1:603756/151‑1 (MQ=255)
                                                                                             ggTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTAc                                      >  2:613043/1‑151 (MQ=255)
                                                                                              gTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACt                                     <  1:40412/151‑1 (MQ=255)
                                                                                               tAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACtt                                    >  1:150549/1‑151 (MQ=255)
                                                                                                             tCTGTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAAttt                      >  2:276625/1‑151 (MQ=255)
                                                                                                                gTTTTGCATCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAAttt                      <  1:459966/148‑1 (MQ=255)
                                                                                                                       aTCGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTAttt            <  1:744830/151‑1 (MQ=255)
                                                                                                                         cGCAAACTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTAtttt           <  2:49/150‑1 (MQ=255)
                                                                                                                              aCTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCAc     >  1:756812/1‑151 (MQ=255)
                                                                                                                              aCTTCAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCAc     >  1:397405/1‑151 (MQ=255)
                                                                                                                                  cAGTGCATTCTCACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACtgg  >  2:382791/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                          
CTGAGTCATTTTTTATCACCAAACCATTGCCACTGCGATTTACCAGCAATTTTTTAAATGTCGCTTTATTTACCGGAGCAATACCCGCTGGCCGGTAAAACAACTTAATTCTGTTTTGCATCGCAAACTTCAGTGCATTCTTACCTTCTTGCTCTGGACTATTTGGTGGAATATCCAGAACATTCAGATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGG  >  NC_000913/2191099‑2191378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: