Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,236,037 G→A G258D (GGT→GAT)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,236,0370GA98.0% 188.1 / ‑4.2 51G258D (GGT→GAT) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (26/24);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

TCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGATC  >  NC_000913/2235890‑2236183
                                                                                                                                                   |                                                                                                                                                  
tCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGatat                                                                                                                                                 >  2:388289/1‑151 (MQ=255)
 cGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATc                                                                                                                                                >  1:504553/1‑151 (MQ=255)
  gATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcg                                                                                                                                               >  2:358397/1‑151 (MQ=255)
   aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcgg                                                                                                                                              >  1:611847/1‑151 (MQ=255)
       tAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcggcg                                                                                                                                            <  1:133197/149‑1 (MQ=255)
                       aTGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGggag                                                                                                                           <  1:567354/150‑1 (MQ=255)
                          ccGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATg                                                                                                                       >  1:296676/1‑151 (MQ=255)
                            gATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGCTGCg                                                                                                                     >  2:267109/1‑151 (MQ=255)
                               cGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGc                                                                                                                   <  2:231825/150‑1 (MQ=255)
                                   aaCGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGt                                                                                                              >  1:404364/1‑151 (MQ=255)
                                        aaaaTCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTa                                                                                                         <  1:776793/151‑1 (MQ=255)
                                                 tATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgc                                                                                                >  2:345118/1‑151 (MQ=255)
                                                  aTTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgc                                                                                                <  2:98696/150‑1 (MQ=255)
                                                            atTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGtt                                                                                     <  2:678579/151‑1 (MQ=255)
                                                               ccGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACa                                                                                  <  2:282001/151‑1 (MQ=255)
                                                                cGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTAc                                                                                   >  1:593304/1‑149 (MQ=255)
                                                                      aGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGacc                                                                           >  2:235473/1‑151 (MQ=255)
                                                                       gCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGacc                                                                           >  1:72678/1‑150 (MQ=255)
                                                                        cGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGaccac                                                                         >  2:449930/1‑151 (MQ=255)
                                                                        cGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGaccac                                                                         >  1:523717/1‑151 (MQ=255)
                                                                                 cGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGcatcat                                                                >  1:738866/1‑151 (MQ=255)
                                                                                  gATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATg                                                               >  1:436414/1‑151 (MQ=255)
                                                                                       cccTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGCTGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAg                                                            >  1:503113/1‑149 (MQ=255)
                                                                                       cccTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACTGGCATCATGCAGTa                                                          <  2:757210/151‑1 (MQ=255)
                                                                                         cagcgcTTCATTCAGCAAATGCGCACCCATCCAGAACGGCGCGATTTGCCAATCGGCGAGATCGGCGGCATTGAAACCTGGGAGAAGGCGGCTAAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCCTCATGCATTACg                                                        <  1:479760/149‑1 (MQ=255)
                                                                                             gcTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTa                                                    <  2:576598/151‑1 (MQ=255)
                                                                                               ttcattcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCCTTGCAACCTGGGAGGATGCGGCTGAGCTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATc                                                  >  1:413018/1‑151 (MQ=255)
                                                                                                tcattcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCg                                                 >  2:332202/1‑151 (MQ=255)
                                                                                                tcattcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGAAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTCACAGGTGACCACCGGCATCATGCAGTACGGGTAt                                                   <  1:14933/149‑1 (MQ=255)
                                                                                                    tcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGAt                                              >  1:616551/1‑150 (MQ=255)
                                                                                                    tcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGCATCGGAt                                              >  2:693731/1‑150 (MQ=255)
                                                                                                     caGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAg                                            <  1:3620/151‑1 (MQ=255)
                                                                                                      aGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGt                                           >  2:443656/1‑151 (MQ=255)
                                                                                                         aaaTGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTgg                                         >  1:666836/1‑150 (MQ=255)
                                                                                                         aaaTGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGa                                        <  2:657585/151‑1 (MQ=255)
                                                                                                             gcgcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGa                                     <  2:569304/150‑1 (MQ=255)
                                                                                                               gcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGatat                                  <  2:703318/151‑1 (MQ=255)
                                                                                                                cACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATg                                 <  1:533332/151‑1 (MQ=255)
                                                                                                                 aCCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATgg                                <  2:40548/151‑1 (MQ=255)
                                                                                                                 aCCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATgg                                <  2:139042/151‑1 (MQ=255)
                                                                                                                 aCCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATgg                                >  2:175396/1‑151 (MQ=255)
                                                                                                                  cccATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGc                               >  2:372070/1‑151 (MQ=255)
                                                                                                                  cccATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAATGGAAGATATgg                                <  2:487117/150‑1 (MQ=255)
                                                                                                                      tCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAg                            >  2:617820/1‑150 (MQ=255)
                                                                                                                         aGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAACggg                        >  1:3360/1‑151 (MQ=255)
                                                                                                                               gcgcGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCg                  <  2:678466/151‑1 (MQ=255)
                                                                                                                                      ttCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTAt            <  1:44595/150‑1 (MQ=255)
                                                                                                                                        cccAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTAtctc         <  2:81669/151‑1 (MQ=255)
                                                                                                                                         ccAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGGTACAGGAGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATCTGGCAAGCGGGTTGTCGCATTAtctc         >  1:259411/1‑150 (MQ=255)
                                                                                                                                              cAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGAt   >  2:195364/1‑151 (MQ=255)
                                                                                                                                              cAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGAt   <  2:203462/151‑1 (MQ=255)
                                                                                                                                               aGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGATc  <  1:319487/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGATC  >  NC_000913/2235890‑2236183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑