Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,243,790	T→C	intergenic (‑138/‑120)	folE ← / → yeiG	GTP cyclohydrolase I/S‑formylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,243,790	0	T	C	100.0%	113.4 / NA	33	intergenic (‑138/‑120)	folE/yeiG	GTP cyclohydrolase I/S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/19);  total (14/19)

CATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCTGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCT  >  NC_000913/2243650‑2243934                                                                                                                                             |                                                                                                                                                 cATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtcatc                                                                                                                                        <  2:210278/151‑1 (MQ=255)  aTTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtcatca                                                                                                                                       <  2:216511/151‑1 (MQ=255)        gATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGa                                                                                                                                  <  2:415098/150‑1 (MQ=255)              tcCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTaa                                                                                                                            >  2:563289/1‑150 (MQ=255)                    aGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCg                                                                                                                     >  1:455204/1‑151 (MQ=255)                      gtgtGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGtt                                                                                                                   >  2:201346/1‑151 (MQ=255)                          gACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGc                                                                                                               <  2:251439/151‑1 (MQ=255)                              ccTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAAt                                                                                                           <  2:463214/151‑1 (MQ=255)                                 ccGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACg                                                                                                        <  1:189167/151‑1 (MQ=255)                                  cGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACgg                                                                                                       <  2:759679/151‑1 (MQ=255)                                     ccTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGa                                                                                                      >  2:10460/1‑149 (MQ=255)                                     ccTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAg                                                                                                    <  1:644731/151‑1 (MQ=255)                                         cGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGaa                                                                                                <  1:96397/151‑1 (MQ=255)                                         cGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGa                                                                                                 >  2:321811/1‑150 (MQ=255)                                             ggggCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGtt                                                                                            <  1:190606/151‑1 (MQ=255)                                                        attaAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGcac                                                                                 <  2:347254/151‑1 (MQ=255)                                                                   ttACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAAc                                                                      >  2:86899/1‑151 (MQ=255)                                                                        gCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTat                                                                 <  2:460097/151‑1 (MQ=255)                                                                         ccTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTatg                                                                <  1:756019/151‑1 (MQ=255)                                                                               tattGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTAtgatga                                                            >  1:749470/1‑149 (MQ=255)                                                                                attGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATgaaga                                                         <  2:99559/151‑1 (MQ=255)                                                                                attGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATgaag                                                          <  1:605784/150‑1 (MQ=255)                                                                                attGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATgaag                                                          <  1:493193/150‑1 (MQ=255)                                                                                         gAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCaa                                                 >  1:664137/1‑150 (MQ=255)                                                                                              ggcgCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAaacaac                                            >  1:704181/1‑150 (MQ=255)                                                                                                 gcACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAacaacaca                                         >  2:271423/1‑150 (MQ=255)                                                                                                   acaTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAacaacacaac                                       >  2:735523/1‑150 (MQ=255)                                                                                                    caTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACAcaacaa                                     <  2:611288/151‑1 (MQ=255)                                                                                                     aTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAACAAACGGTATGATGAAGAAATTGCAAACAACAcaacaat                                    <  1:310972/151‑2 (MQ=255)                                                                                                         tCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGc                                >  2:220328/1‑151 (MQ=255)                                                                                                              tATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGc                           >  2:485628/1‑151 (MQ=255)                                                                                                                     aGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGaa                     >  2:274218/1‑150 (MQ=255)                                                                                                                       cGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATg                  <  2:713047/151‑1 (MQ=255)                                                                                                                                        gccgccgccTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCt  <  1:516161/150‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                 CATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCTGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCT  >  NC_000913/2243650‑2243934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑